Structure basis of reaction mechanism of beta-lactamases and new inhibitor search
| Project/Area Number |
26460534
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Bacteriology (including mycology)
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| Research Institution | Josai International University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
HOSHINO Tyuji 千葉大学, 薬学研究院, 准教授 (90257220)
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| Research Collaborator |
James R Knox Univ. of Connecticut, Professor
Robert Bonomo Research Service, Louis Stokes Cleveland Department of Veterans Affairs, Professor
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 薬剤耐性 / 抗生物質 / 感染症 / 蛋白質 / 構造生物学 / X線結晶解析 / β-ラクタマーゼ / タンパク質 / β-ラクタム剤 / 分子動力学計算 |
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| Outline of Final Research Achievements |
beta-Lactamases are the main cause of bacterial beta-lactams resistant.However, the emergence of novel beta-lactamases with direct carbapenem-hydrolyzing activity (carbapenemase) has contributed to an increased prevalence of carbapenem-resistant Enterobacteriaceae (CRE). In this study, we studied three subjects, which are concerned with beta-lactamase inhibition mechanism by carbapenems or carbapenemase inhibitor, avibactam.
(1) PenA class A carbapenemase PenA complex with avibactam. (2) Fox-4 plasmid mediated class C beta-lactamase in complex with avibactam. (3) Enterobacter cloacae P99 class C beta-lactamase in complex with biapenem.
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Report
(4 results)
Research Products
(7 results)
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[Journal Article] Overcoming an Extremely Drug Resistant (XDR) Pathogen: Avibactam Restores Susceptibility to Ceftazidime for Burkholderia cepacia Complex Isolates from Cystic Fibrosis Patients.2017
Author(s)
Papp-Wallace KM, Becka SA, Zeiser ET, Ohuchi N, Mojica MF, Gatta JA, Falleni M, Tosi D, Borghi E, Winkler ML, Wilson BM, LiPuma JJ, Nukaga M, Bonomo RA.
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Journal Title
ACS Infect Dis.
Volume: 印刷中
Issue: 7
Pages: 502-511
DOI
Related Report
Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
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[Presentation] Exploring the Inactivation Mechanism by Avibactam (AVI) of an Inhibitor-Resistant Carbapenemase, PenA from Burkholderia multivorans (Bm)2015
Author(s)
K. M. Papp-Wallace, J. A. Gatta, N. Ohuchi, M. L. Winkler, S. A. Becka, J. J. LiPuma, R. A. Bonomo, M. Nukaga
Organizer
Interscience Conference of Antimicrobial Agents and Chemotherapy 2015,San Diego, CA
Place of Presentation
San Diego Convention Center
Year and Date
2015-09-21
Related Report
Int'l Joint Research
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