| Project/Area Number |
26460537
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Bacteriology (including mycology)
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| Research Institution | Kyorin University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
神谷 茂 杏林大学, 医学部, 教授 (10177587)
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| Co-Investigator(Renkei-kenkyūsha) |
YAMAMOTO Tomoko 千葉大学, 薬学研究科, 教授 (60110342)
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| Research Collaborator |
Armstrong Sandra K. ミネソタ大学, 医学研究科, 教授
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 百日咳菌 / 緊縮応答 / 遺伝子発現 / ３型分泌装置 / 栄養枯渇 / グルタミン酸 / アラモン / ３型分泌機構 / バイオフィルム / ppGpp / 3型分泌装置 / 病原性 |
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| Outline of Final Research Achievements |
In bacterial cells, nutritional starvation causes accumulation of (p)ppGpp which regulate various gene expressions. In the present study, we assessed the effect of (p)ppGpp and glutamate limitation to investigate its role in the regulation of pathogenesis.
When glutamate was depleted, Bordetella pertussis accumulated (p)ppGpp and elevated mRNA of type 3 secretion system genes. On the other hand, the mRNA level of btrS which encodes transcription of T3SS genes were not altered. Therefore, (p)ppGpp probably affect the transcription by BtrS. Glutamate limitation increased survival rate within macrophages and lack of (p)ppGpp decreased it. Attachment to macrophages or epithelial cells were not affected by these conditions. Accordingly, it is suggested that (p)ppGpp have some role in the interaction to macrophages in B. pertussis.
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