The mechanism by which influenza C virus NS1 protein regulates virus replication
| Project/Area Number |
26460547
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Virology
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| Research Institution | Yamagata University |
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Principal Investigator |
Hongo Seiji 山形大学, 医学部, 教授 (90229245)
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| Co-Investigator(Kenkyū-buntansha) |
下平 義隆 山形大学, 医学部, 助教 (30445746)
村木 靖 岩手医科大学, 医学部, 教授 (00241688)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | C型インフルエンザ / ウイルス / NS1 / 核移行シグナル / 核外移行シグナル / ＮＳ１ / スプライシング / 細胞内局在 |
|---|
| Outline of Final Research Achievements |
Influenza C virus NS1 protein localized in the nucleus and cytoplasm in the early and late phase of infection, respectively, suggested that the NS1 protein may possess a nuclear localization signal (NLS) and a nuclear export signal (NES). We analyzed the deduced amino acid sequences of the NS1 and found that two overlapping leucine-rich NES-like sequences are present at residues 109-126, and that four basic amino acid stretches are present at residues 36-46, 87-90, 167-171, and 189-196 as the candidates for NLSs. The nuclear export of the NS1 mutant which lost residues 109-126 was severely impaired, suggesting that NES is present at residues 109-126. The nuclear import of the NS1 mutant which lost residues 167-171 or 189-196 was severely impaired, suggesting that NLS is present at residues 167-171 and 189-196.
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Report
(4 results)
Research Products
(23 results)
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[Journal Article] The serine protease inhibitor camostat inhibits influenza virus replication and cytokine production in primary cultures of human tracheal epithelial cells.2015
Author(s)
Yamaya M, Shimotai Y, Hatachi Y, Lusamba Kalonji N, Tando Y, Kitajima Y, Matsuo K, Kubo H, Nagatomi R, Hongo S, Homma M, Nishimura H
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Journal Title
Pulm Pharmacol Ther
Volume: 33
Pages: 66-74
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Epitope Mapping of the Hemagglutinin Molecule of A/(H1N1)pdm09 Virus by Using Monoclonal Antibody Escape Mutants2014
Author(s)
Yoko Matsuzaki, Kanetsu Sugawara., Mina Nakauchi, Yoshimasa Takahashi, Taishi Onodera, Yasuko Tsunetsugu-Yokota, Takayuki Matsumura, Manabu Ato, Kazuo Kobayashi, Yoshitaka Shimotai, Katsumi Mizuta, Seiji Hongo, Masato Tashiro, Eri Nobusawa
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Journal Title
J. Virol.
Volume: 88
Issue: 21
Pages: 12364-12373
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Epidemiological information regarding the periodic epidemics of influenza C virus in Japan (1996-2013) and the seroprevalence of antibodies to different antigenic groups.2014
Author(s)
Matsuzaki Y, Sugawara K, Abiko C, Ikeda T, Aoki Y, Mizuta K, Katsushima N, Katsushima F, Katsushima Y, Itagaki T, Shimotai Y, Hongo S, Muraki Y, Nishimura H
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Journal Title
J Clin Virol
Volume: 61
Issue: 1
Pages: 87-93
DOI
Related Report
Peer Reviewed / Open Access
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