Further analysis of phosphorylationin events in herpes simplex virus infection
| Project/Area Number |
26460548
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Virology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
Akihisa Kato 東京大学, 医科学研究所, 助教 (40581187)
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| Co-Investigator(Kenkyū-buntansha) |
川口 寧 東京大学, 医科学研究所, 教授 (60292984)
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| Project Period (FY) |
2014-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | HSV / VP26 / Us8A / 神経病原性 / 神経侵襲性 / リン酸化プロテオーム解析 / UL51 / 小胞媒介性核外輸送 / リン酸化制御機構 / vUNG / Conserved Serine / 単純ヘルペスウイルス / UL12 |
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| Outline of Final Research Achievements |
Herpes simplex virus type (HSV) causes a range of human diseases. HSV has evolved mechanisms to utilize the phosphorylation system for the regulation of their own viral proteins and to establish a cellular environment for efficient viral replication and virulence. However, our knowledge of them remains to be limited and fragmented. In this study, we identified novel phosphorylation sites of small capsid protein VP26 and putative membrane protein Us8A by mass spectrometry-based phosphoproteomic analysis of HSV-infected cells. Our study showed that (i) the phosphorylation of VP26 Thr-111 is required for both efficient HSV-1 replication and cell-cell spread in SK-N-SH cells and HSV-1 neurovirulence in mice by regulating localazaton of VP26 and its binding partner, the major capsid protein VP5, (ii) the Us3-mediated phosphorylation of Us8A Ser-61 regulates Us8A function for viral invasion into the CNS from peripheral sites.
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Report
(5 results)
Research Products
(117 results)
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[Journal Article] Herpes Simplex Virus 1 VP22 Inhibits AIM2-Dependent Inflammasome Activation to Enable Efficient Viral Replication.2018
Author(s)
Maruzuru Y, Ichinohe T, Sato R, Miyake K, Okano T, Suzuki T, Koshiba T, Koyanagi N, Tsuda S, Watanabe M, Arii J, Kato A, Kawaguchi Y.
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Journal Title
Cell Host Microbe.
Volume: 23
Issue: 2
Pages: 254-265
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Herpes simplex virus-1 evasion of CD8+ T cell accumulation contributes to viral encephalitis.2017
Author(s)
Koyanagi N, Imai T, Shindo K, Sato A, Fujii W, Ichinohe T, Takemura N, Kakuta S, Uematsu S, Kiyono H, Maruzuru Y, Arii J, Kato A, Kawaguchi Y.
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Journal Title
J Clin Invest.
Volume: 127
Issue: 10
Pages: 3784-3795
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Rapid Screening by Cell-Based Fusion Assay for Identifying Novel Antivirals of Glycoprotein B-Mediated Herpes Simplex Virus Type 1 Infection2016
Author(s)
Maeda N, Furukawa A, Kakita K, Anada M, Hashimoto S, Matsunaga S, Kuroki K, Ose T, Kato A, Arii J, Kawaguchi Y, Arase H, Maenaka K
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Journal Title
Biological and Pharmaceutical Bulletin
Volume: 39
Issue: 11
Pages: 1897-1902
DOI
NAID
ISSN
0918-6158, 1347-5215
Related Report
Peer Reviewed / Open Access
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[Journal Article] Long Noncoding RNA NEAT1-Dependent SFPQ Relocation from Promoter Region to Paraspeckle Mediates IL8 Expression upon Immune Stimuli2014
Author(s)
Imamura K, Imamachi N, Akizuki G, Kumakura M, Kawaguchi A, Nagata K, Kato A, Kawaguchi Y, Sato H, Yoneda M, Kai C, Yada T, Suzuki Y, Yamada T, Ozawa T, Kaneki K, Inoue T, Kobayashi M, Kodama T, Wada Y, Sekimizu K and Akimitsu N.
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Journal Title
Molecular cell
Volume: 53
Issue: 3
Pages: 393-406
DOI
Related Report
Peer Reviewed / Open Access
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[Presentation] ESCRT-III mediates HSV-1 Nuclear Egress2016
Author(s)
Jun Arii, Fumio Maeda, Akihisa Kato, Yasushi Kawaguchi
Organizer
41st Annual International Herpesvirus Workshop
Place of Presentation
Monona Terrace Community and Convention Center (Madison, Wisconsin, USA)
Year and Date
2016-07-23
Related Report
Int'l Joint Research
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[Presentation] Identification of a host cell protein, RanBP10, which acts like ICP0 in HSV-1-infected cells2015
Author(s)
Yuka Sato, Akihisa Kato, Yuhei Maruzuru, Yoshitaka Hirohata, Shumpei Tsuda, Jun Arii, Yasushi Kawaguchi
Organizer
The 40th Annual international Herpesvirus Workshop
Place of Presentation
The Boise Centre (Boise, Idaho, USA)
Year and Date
2015-07-25
Related Report
Int'l Joint Research
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[Presentation] RanBP10, a novel host cellular protein that interacts with a herpes simplex virus 1 (HSV-1) regulatory protein ICP0, regulates viral gene expression by acting like ICP0 in HSV-1-infected cells2015
Author(s)
Yuka Sato, Akihisa Kato, Yuhei Maruzuru, Yoshitaka Hirohata, Shumpei Tsuda, Jun Arii, Yasushi Kawaguchi
Organizer
The 14th Awaji International Forum on Infection and Immunity
Place of Presentation
Awaji Yumebutai Conference Center(Awaji city, Hygo, Japan)
Year and Date
2015-07-25
Related Report
Int'l Joint Research
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[Presentation] Herpes Simplex Virus 1 ICP22 Interacts with Viral Nuclear Egress factors UL31, UL34 and Us3, and Regulates Viral Nuclear Egress2015
Author(s)
Yuhei Maruzuru, Keiko Shindo, Zhuoming Liu, Masaaki Oyama, Hiroko Kozuka-Hata, Jun Arii, Akihisa Kato, Yasushi Kawaguchi
Organizer
The 14th Awaji International Forum on Infection and Immunity
Place of Presentation
Awaji Yumebutai Conference Center(Awaji city, Hygo, Japan)
Year and Date
2015-07-25
Related Report
Int'l Joint Research
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[Presentation] Herpes Simplex Virus 1 Recruits CD98 Heavy Chain and β1 Integrin to the Nuclear Membrane for Viral De-Envelopment2015
Author(s)
Yoshitaka Hirohata, Jun Arii, Zhuoming Liu, Keiko Shindo, Masaaki Oyama, Hiroko Kozuka-Hata, Hiroshi Sagara, Akihisa Kato, Yasushi Kawaguchi
Organizer
The 14th Awaji International Forum on Infection and Immunity
Place of Presentation
Awaji Yumebutai Conference Center(Awaji city, Hygo, Japan)
Year and Date
2015-07-25
Related Report
Int'l Joint Research
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