Recovery of functional suspend of T-cells membrane lipid raft caused by HIV using anti-myristoylation diabody.
Project/Area Number |
26460551
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Virology
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Research Institution | Tokyo Institute of Technology |
Principal Investigator |
|
Project Period (FY) |
2014-04-01 – 2017-03-31
|
Project Status |
Completed (Fiscal Year 2016)
|
Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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Keywords | ミリストイル化 / 抗体 / HIV / AIDS / membrane lipid raft / 抗体ライブラリ / エイズ / Nef / 抗体ライブラリー / プロテオミクス / 生体膜脂質ラフト / HIV |
Outline of Final Research Achievements |
Addition of original anti-myristoylation antibody reduced precipitation of myristoylated HIV-Nef in the presence of lipid micelle, and this result indicates inhibitory effect of the antibody restraining interaction between myristoylated molecules and lipid membranes. Next, anti HIV-Nef antibody with capacities of high specificity and low dissociation constant (10^-9) was developed by original antibody library technique. A diabody was constructed by a fusion of the antibody with the anti- myristoylation antibody. Addition of the diabody preliminary reduced precipitation of myristoylated HIV-Nef in the presence of lipid micelle again. The remaining subject is specificity of the diabody to myristoylated proteins in the cells.
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Report
(4 results)
Research Products
(1 results)