| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
|
|---|
| Outline of Final Research Achievements |
Addition of original anti-myristoylation antibody reduced precipitation of myristoylated HIV-Nef in the presence of lipid micelle, and this result indicates inhibitory effect of the antibody restraining interaction between myristoylated molecules and lipid membranes.
Next, anti HIV-Nef antibody with capacities of high specificity and low dissociation constant (10^-9) was developed by original antibody library technique.
A diabody was constructed by a fusion of the antibody with the anti- myristoylation antibody. Addition of the diabody preliminary reduced precipitation of myristoylated HIV-Nef in the presence of lipid micelle again. The remaining subject is specificity of the diabody to myristoylated proteins in the cells.
|
