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Analysis of functional and physical HN-F interaction in the course of membrane fusion by paramyxoviruses

Research Project

Project/Area Number 26460552
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Virology
Research InstitutionMie University

Principal Investigator

Tsurudome Masato  三重大学, 医学系研究科, 准教授 (50159042)

Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywordsパラインフルエンザウイルス / HN蛋白 / F蛋白 / 分子間相互作用 / 膜融合 / 細胞融合
Outline of Final Research Achievements

It is widely appreciated that during the course of membrane fusion by parainfluenza viruses, the head domain of the viral envelope protein HN (hemagglutinin-neuraminidase) undergoes a structural transition after its binding to the receptors on the cell surface, that exposes otherwise hidden stalk domain of the HN. The exposed HN stalk domain then interacts with the head domain of another viral envelope protein F (fusion) in a virus-specific manner, which enables the F to undergo a series of structural changes that lead to merger of viral and cellular membranes.
Such specificity is believed to be defined by the similarity in the primary structures of the HN stalk domain and the F head domain between different viruses. However, our present study revealed that the HN head domain and F stalk domain can modulate the specificity by affecting tertiary and/or quaternary structures of the HN stalk domain and the F head domain, respectively.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (24 results)

All 2017 2016 2015 2014

All Journal Article (8 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 8 results,  Open Access: 6 results,  Acknowledgement Compliant: 3 results) Presentation (16 results) (of which Int'l Joint Research: 2 results)

  • [Journal Article] Graf1 controls the growth of human parainfluenza virus type 2 through inactivation of RhoA signaling.2016

    • Author(s)
      Ohta K, Goto H, Matsumoto Y, Yumine N, Tsurudome M, Nishio M
    • Journal Title

      Journal of Virology

      Volume: 90 Issue: 20 Pages: 9394-9405

    • DOI

      10.1128/jvi.01471-16

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Enhanced growth of influenza A virus by coinfection with human parainfluenza virus type 22016

    • Author(s)
      Goto H, Ihira H, Morishita K, Tsuchiya M, Ohta K, Yumine N, Tsurudome M, Nishio M
    • Journal Title

      Medical Microbiology and Immunology

      Volume: 205 Pages: 1-10

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Enhanced growth of influenza A virus by coinfection with human parainfluenza virus type 22015

    • Author(s)
      Goto H, Ihira H, Morishita K, Tsuchiya M, Ohta K, Yumine N, Tsurudome M, Nishio M
    • Journal Title

      Med Microbiol Immunol

      Volume: - Issue: 3 Pages: 209-218

    • DOI

      10.1007/s00430-015-0441-y

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Pathogenesis of the Influenza Virus in Diabetes:Model Mice2015

    • Author(s)
      Morihiro Ito, Hiromichi Kubota, Tamaki Takeuchi, Shanlou Qiao, Masato Tsurudome, Hideki Tsumura, Akio Matsuda, Machiko Nishio and Yasuhiko Ito
    • Journal Title

      British Microbiology Research Journal

      Volume: 9 Issue: 6 Pages: 1-7

    • DOI

      10.9734/bmrj/2015/18799

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] The fusion protein specificity of the parainfluenza virus hemagglutinin-neuraminidase protein is not solely defined by the primary structure of its stalk domain2015

    • Author(s)
      Tsurudome M, Ito M, Ohtsuka J, Hara K, Komada H, Nishio M, Nosaka T
    • Journal Title

      Journal of Virology

      Volume: 89 Issue: 24 Pages: 12374-12387

    • DOI

      10.1128/jvi.01448-15

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Vero/BC-F: an efficient packaging cell line stably expressing F protein to generate single round-infectious human parainfluenza virus type 2 vector.2014

    • Author(s)
      Ohtsuka J, Fukumura M, Tsurudome M, Hara K, Nishio M, Kawano M, Nosaka T.
    • Journal Title

      Gene Ther

      Volume: 21 Issue: 8 Pages: 775-784

    • DOI

      10.1038/gt.2014.55

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Inhibition of human parainfluenza virus type 2 growth in vitro by catechin is caused by the inhibition of genome and mRNA synthesis and by the disruption of cytoskeleton, and that by tannic acid is mainly caused by genome synthesis inhibition and the disruption of cytoskeleton2014

    • Author(s)
      Uematsu J, Kihira S, Uchida M, Sakakura Y, Deguchi S, Sakai K, Yamamoto H, Kawano M, Tsurudome M, O'Brien M, Komada H
    • Journal Title

      International Journal of Sciences

      Volume: 3 Pages: 47-55

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Ribavirin inhibits human parainfluenza virus type 2 replication in vitro.2014

    • Author(s)
      Kihira S, Uematsu J, Kawano M, Itoh A, Ookohchi A, Satoh S, Maeda Y, Sakai K, Yamamoto H, Tsurudome M, O'Brien M, Komada H.
    • Journal Title

      Microbiol Immunol.

      Volume: 58 Issue: 11 Pages: 628-635

    • DOI

      10.1111/1348-0421.12192

    • NAID

      40020271490

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] フィトヘムアグルチニンによるヒトパラインフルエンザウイルス2型増殖への影響2017

    • Author(s)
      植松 淳、酒井香江、山本秀孝、河野光雄、鶴留雅人、駒田 洋
    • Organizer
      日本薬学会第137年会
    • Place of Presentation
      仙台国際センター, 仙台, 宮城
    • Year and Date
      2017-03-24
    • Related Report
      2016 Annual Research Report
  • [Presentation] The F protein specificity of the parainfluenza virus sHN protein can be modified by substituting specified amino acids in the HN head domain.2016

    • Author(s)
      Tsurudome M, Ito M, Nishio M, K, Nosaka T
    • Organizer
      The 64th Annual Meeting of the Japanese Society for Virology
    • Place of Presentation
      札幌コンベンションセンター, 札幌, 北海道
    • Year and Date
      2016-10-23
    • Related Report
      2016 Annual Research Report
  • [Presentation] Rab27a involves the growth of human parainfluenza virus type 22016

    • Author(s)
      Ohta K, Matsumoto Y, Yumine N, Tsurudome M, Nishio M
    • Organizer
      The 64th Annual Meeting of the Japanese Society for Virology
    • Place of Presentation
      札幌コンベンションセンター, 札幌, 北海道
    • Year and Date
      2016-10-23
    • Related Report
      2016 Annual Research Report
  • [Presentation] ミコフェノール酸によるヒトパラインフルエンザウイルス2型増殖阻害2016

    • Author(s)
      植松 淳、酒井香江、山本秀孝、河野光雄、鶴留雅人、駒田 洋
    • Organizer
      日本薬学会第136年会
    • Place of Presentation
      パシフィコ横浜(神奈川県・横浜市)
    • Year and Date
      2016-03-26
    • Related Report
      2015 Research-status Report
  • [Presentation] Factors that define the F protein specificity of the parainfluenza virus HN protein2015

    • Author(s)
      Tsurudome M, Ito M, Nishio M, Komada H, Nosaka T
    • Organizer
      The 63rd Annual Meeting of the Japanese Society for Virology
    • Place of Presentation
      福岡国際会議場(福岡県・福岡市)
    • Year and Date
      2015-11-22
    • Related Report
      2015 Research-status Report
  • [Presentation] Graf1/ARHGAP26 negatively regulates the growth of human parainfluenza virus type 2 through the RhoA inactivation2015

    • Author(s)
      Ohta K, Goto H, Matsumoto Y, Yumine N, Tsurudome M, Nishio M
    • Organizer
      The 63rd Annual Meeting of the Japanese Society for Virology
    • Place of Presentation
      福岡国際会議場(福岡県・福岡市)
    • Year and Date
      2015-11-22
    • Related Report
      2015 Research-status Report
  • [Presentation] Analysis of fusion-inducing ability of mumps virus clinical isolates2015

    • Author(s)
      Ito M, Yoshikawa H, Shigetomi R, Sato Y, Takeuchi T, Ihara T, Tsurudome M, Hayashi S, ItoY
    • Organizer
      The 63rd Annual Meeting of the Japanese Society for Virology
    • Place of Presentation
      福岡国際会議場(福岡県・福岡市)
    • Year and Date
      2015-11-22
    • Related Report
      2015 Research-status Report
  • [Presentation] ミコフェノール酸のパラインフルエンザ2型への抗ウイルス作用2015

    • Author(s)
      駒田 洋、酒井香江、植松 淳、山本秀孝、河野光雄、鶴留雅人
    • Organizer
      日本病院薬剤師会東海ブロック 日本薬学会東海支部合同学術大会 2015
    • Place of Presentation
      金城学院大学(愛知県・名古屋市)
    • Year and Date
      2015-11-01
    • Related Report
      2015 Research-status Report
  • [Presentation] The F specificity of the parainfluenza virus HN is not solely defined by the primary structure of the HN stalk domain2015

    • Author(s)
      Tsurudome M, Ito M, Nishio M, Nosaka T
    • Organizer
      XVI International Conference on Negative Strand Viruses
    • Place of Presentation
      Auditorium Department of Law University deli Studi di Siena, Italy
    • Year and Date
      2015-06-14
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Involvement of Graf1/ARHGAP26 and Rho family GTPases in the growth of human parainfluenza virus type 22015

    • Author(s)
      Ohta K, Goto H, Matsumoto Y, Yumine N, Tsurudome M, Nishio M
    • Organizer
      XVI International Conference on Negative Strand Viruses
    • Place of Presentation
      Auditorium Department of Law University deli Studi di Siena, Italy
    • Year and Date
      2015-06-14
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] カスパーゼ阻害剤Z-VAD-FMK酸のパラインフルエンザウイルス2型増殖への作用2015

    • Author(s)
      植松 淳、酒井香江、鈴木沙織、吉美由美子、山本秀孝、河野光雄、鶴留雅人、駒田 洋
    • Organizer
      日本薬学会第135年会
    • Place of Presentation
      デザイン・クリエイティブセンター 神戸
    • Year and Date
      2015-03-25 – 2015-03-28
    • Related Report
      2014 Research-status Report
  • [Presentation] フラボノイドのパラインフルエンザウイルス2型増殖への作用2015

    • Author(s)
      酒井香江、植松 淳、山本秀孝、河野光雄、鶴留雅人、駒田 洋
    • Organizer
      日本薬学会第135年会
    • Place of Presentation
      デザイン・クリエイティブセンター神戸
    • Year and Date
      2015-03-25 – 2015-03-28
    • Related Report
      2014 Research-status Report
  • [Presentation] パラインフルエンザウイルスのHNとFの機能的相互作用の分子機構: Fとの相互作用におけるHNのHead領域の役割2014

    • Author(s)
      鶴留雅人, 伊藤守弘, 大塚順平, 駒田 洋, 西尾真智子, 野阪哲哉
    • Organizer
      第62回日本ウイルス学会学術集会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2014-11-10 – 2014-11-12
    • Related Report
      2014 Research-status Report
  • [Presentation] ムンプスウイルスの臨床分離株の細胞融合誘導能の解析2014

    • Author(s)
      伊藤守弘, 吉川大貴, 久保田弘通, 迫田兼人, 竹内 環, 庵原俊昭, 鶴留雅人, 伊藤康彦
    • Organizer
      第62回日本ウイルス学会学術集会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2014-11-10 – 2014-11-12
    • Related Report
      2014 Research-status Report
  • [Presentation] パラミクソウイルスの細胞融合誘導におけるCD98の関与2014

    • Author(s)
      久保田弘通, 伊藤守弘, 吉川大貴, 迫田兼人, 竹内 環, 鶴留雅人, 駒田 洋, 西尾真智子, 伊藤康彦
    • Organizer
      第62回日本ウイルス学会学術集会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2014-11-10 – 2014-11-12
    • Related Report
      2014 Research-status Report
  • [Presentation] 抗ウイルス因子Thetherin がパラミクソウイルスの増殖に与える影響2014

    • Author(s)
      太田圭介, 後藤秀男, 松本祐介, 湯峯奈都子, 鶴留雅人, 西尾真智子
    • Organizer
      第62回日本ウイルス学会学術集会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2014-11-10 – 2014-11-12
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2018-03-22  

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