Host Factors involved in the formation of Flavivirus replication organelle
| Project/Area Number |
26460555
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Virology
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| Research Institution | Hirosaki University |
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Principal Investigator |
Morita Eiji 弘前大学, 農学生命科学部, 准教授 (70344653)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
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| Keywords | フラビウイルス / ESCRT / 宿主因子 / デングウイルス / 日本脳炎ウイルス / VCP / 複製オルガネラ / オートファジー / ウイルス |
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| Outline of Final Research Achievements |
Flavivirus, including dengue virus and Japanese encephalitis virus (JEV), infection induces endoplasmic reticulum (ER) membrane rearrangements to crate a compartment for replication of the viral genome and assembly of viral particles. By extensive mass spectrometry analysis, we identified ESCRT (endosomal sorting complex required for transport) factors that are recruited to sites of virus replication. Systematic siRNA analysis revealed that release of JEV was dramatically decreased by single depletion of TSG101 or co-depletion of specific combinations of CHMP family proteins. By contrast, release was unaffected by depletion of some core ESCRTs, including VPS4. Thus, a unique subset of ESCRT contributes to ER membrane biogenesis during flavivirus infection. This unique ESCRT requirement implies the existence of ER-specific features for ESCRT-mediated membrane rearrangement, and our study provides the first demonstration of the mechanistic features of ER ESCRTs.
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Report
(4 results)
Research Products
(17 results)
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[Journal Article] Unique Requirement for ESCRT Factors in Flavivirus Particle Formation on the Endoplasmic Reticulum2016
Author(s)
Keisuke Tabata, Masaru Arimoto, Masashi Arakawa, Atsuki Nara, Kazunobu Saito, Hiroko Omori, Arisa Arai, Tomohiro Ishikawa, Eiji Konishi, Ryosuke Suzuki, Yoshiharu Matsuura, Eiji Morita
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Journal Title
Cell Reports
Volume: 16
Issue: 9
Pages: 2339-2347
DOI
Related Report
Peer Reviewed / Open Access
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[Presentation] Involvement of ESCRT factors in Flavivirus propagation2014
Author(s)
Keisuke Tabata, Masaru Arimoto, Kazunobu Saito, Hiroko Omori, Yoshiharu Matsuura, and Eiji Morita
Organizer
Keystone Symposia, The Ins and Outs of Viral Infection: Entry, Assembly, Exit and Spread (C6)
Place of Presentation
Beaver Run Resort, Breckenridge, Colorado, USA
Year and Date
2014-04-01 – 2014-04-04
Related Report
