| Project/Area Number |
26460556
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Virology
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| Research Institution | The University of Tokushima |
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Principal Investigator |
NOMAGUCHI Masako 徳島大学, 大学院医歯薬学研究部(医学系), 教授 (80452647)
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| Co-Investigator(Kenkyū-buntansha) |
宮崎 恭行 徳島大学, 大学院医歯薬学研究部(医学系), 助教 (70607233)
足立 昭夫 徳島大学, 大学院医歯薬学研究部(医学系), 特命教授 (90127043)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | HIV / Vif / APOBEC3G / ウイルス複製 / 変異・適応 / 遺伝子発現 / 選択的スプライシング / 適応・進化 |
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| Outline of Final Research Achievements |
Power balance between HIV-1 Vif and APOBEC3G is critical for viral replication. Through HIV-1 adaptation study, we found that virus replication capacity varies by naturally-occurring single nucleotide polymorphisms (nsSNP) within the region proximal to the splicing acceptor 1 (SA1prox) and SLSA1 structure on the HIV-1 genome. Molecular virological analysis in this study revealed that the change of viral growth ability results from the increase and decrease in Vif expression levels by nsSNPs. Thus, the nucleotide sequence of SA1prox region is important for determining the Vif expression level. To investigate how viruses expressing an extremely low level of Vif adapt themselves to environments with a high APOBEC3G level, we performed virus adaptation experiments. The results showed that the region other than SA1prox is also involved in vif mRNA production, and that HIV-1 displays a high adaptation ability even under circumstances where virus replication is strongly restricted.
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