| Project/Area Number |
26460565
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Virology
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| Research Institution | National Institute of Infectious Diseases |
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Principal Investigator |
Watashi Koichi 国立感染症研究所, ウイルス第二部, 主任研究官 (40378948)
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| Research Collaborator |
Tsukuda Senko 国立感染症研究所, ウイルス第二部, 協力研究員
Iwamoto Masashi 国立感染症研究所, ウイルス第二部, 研究生
Ohashi Hirofumi 国立感染症研究所, ウイルス第二部, 研究生
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| Project Period (FY) |
2014-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 肝炎ウイルス / 翻訳後修飾 / HBV / B型肝炎ウイルス / ウイルス / 肝炎 / 複製 / 化合物 / 微小管 |
|---|
| Outline of Final Research Achievements |
Molecular mechanisms underlying hepatitis B virus (HBV) life cycle has not been fully clarified. In this study, we identified host factors regulating HBV life cycle and analyzed its regulation mechanism by using cell models efficiently supporting HBV infection and replication. Through the identification of nocodazole that inhibited HBV replication, we revealed that cellular microtubules were important for the multimerization of core protein and post-translational modification of tubulin regulated HBV replication. We also identified cellular kinases that regulated HBV entry process. These findings provide information important for understanding the molecular mechanisms of virus-host interaction.
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