Study on local regulation of gene expression of female reproductive-specific IFN

• Project/Area Number: 26460567
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Immunology
• Research Institution: Hirosaki University
• Principal Investigator: MATSUMIYA TOMOH 弘前大学, 医学研究科, 助教 (30344592)
• Co-Investigator(Kenkyū-buntansha): 今泉 忠淳 弘前大学, 医学研究科, 教授 (90232602)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 自然免疫 / 産婦人科 / 感染症 / IFN / 免疫学 / インターフェロン / Importin / インポーチン / ＩＦＮ / importin / 非翻訳領域

Outline of Final Research Achievements

IFN-εis unique reproductive-specific type I IFN. We have shown that importin 9 (IPO9) regulate IFN-ε. In this study, we focused on local regulation of gene expression of IFN-ε. Analysis for 5’ untranslated region of IFN-εmRNA showed that IPO9 unaffected mRNA stability of IFN-ε. In addition we found molecules that can interact with IPO9.

Research Products

• [Journal Article] Interferon (IFN)-induced protein 35 (IFI35) negatively regulates IFN-β-phosphorylated STAT1-RIG-I-CXCL10/CCL5 axis in U373MG astrocytoma cells treated with polyinosinic-polycytidylic acid. (2017)
  • Author(s): Shirai K, Shimada T, Yoshida H, Hayakari R, Matsumiya T, Tanji K, Murakami M, Tanaka H, Imaizumi T.
  • Journal Title: Brain Res. Volume: 1658 Pages: 60-67
  • DOI: 10.1016/j.brainres.2017.01.018
  • Peer Reviewed
• [Journal Article] Non-Canonical Role of IKKα in the Regulation of STAT1 Phosphorylation in Antiviral Signaling. (2016)
  • Author(s): Xing F, Matsumiya T, Shiba Y, Hayakari R, Yoshida H, Imaizumi T.
  • Journal Title: PLoS One. Volume: 11 Issue: 12 Pages: e0168696-e0168696
  • DOI: 10.1371/journal.pone.0168696
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Critical Role of IRF-3 in the Direct Regulation of dsRNA-Induced Retinoic Acid-Inducible Gene-I (RIG-I) Expression. (2016)
  • Author(s): Hayakari R, Matsumiya T, Xing F, Yoshida H, Hayakari M, Imaizumi T.
  • Journal Title: PLoS One. Volume: 11 Issue: 9 Pages: e0163520-e0163520
  • DOI: 10.1371/journal.pone.0163520
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Interferon (IFN)-Induced Protein 35 (IFI35), a Type I Interferon-Dependent Transcript, Upregulates Inflammatory Signaling Pathways by Activating Toll-Like Receptor 3 in Human Mesangial Cells. (2016)
  • Author(s): Imaizumi T, Yano C, Numata A, Tsugawa K, Hayakari R, Matsumiya T, Yoshida H, Watanabe S, Tsuruga K,
  • Journal Title: Kidney Blood Press Res. Volume: 41 Pages: 635-42
  • NAID: 120006557469
  • Peer Reviewed
• [Journal Article] Alteration of Antiviral Signalling by Single Nucleotide Polymorphisms (SNPs) of Mitochondrial Antiviral Signalling Protein (MAVS). (2016)
  • Author(s): Xing F, Matsumiya T, Hayakari R, Yoshida H, Kawaguchi S, Takahashi I, Nakaji S, Imaizumi T.
  • Journal Title: PLoS One Volume: 11 Issue: 3 Pages: e0151173-e0151173
  • DOI: 10.1371/journal.pone.0151173
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Identification of a point mutation in the SH3BP2 gene in cherubism (2015)
  • Author(s): Sakaki H, Matsumiya T, Furudate F, Kobayashi W, Kon T, Itoh R, Kimura H
  • Journal Title: Journal of Oral and Maxillofacial Surgery, Medicine, and Pathology Volume: 27 Issue: 6 Pages: 880-3
  • DOI: 10.1016/j.ajoms.2015.03.007
  • Peer Reviewed
• [Journal Article] Retinoic acid-inducible gene-I-like receptor (RLR)-mediated antiviral innate immune responses in the lower respiratory tract: Roles of TRAF3 and TRAF5. (2015)
  • Author(s): Chiba Y, Matsumiya T, Satoh T, Hayakari R, Furudate K, Xing F, Yoshida H, Tanji K, Mizukami H, Imaizumi T, Ito E.
  • Journal Title: Biochem Biophys Res Commun Volume: 467 Issue: 2 Pages: 191-196
  • DOI: 10.1016/j.bbrc.2015.10.010
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Emerging functional cross-talk between the Keap1-Nrf2 system and mitochondria (2015)
  • Author(s): Itoh K, Ye P, Matsumiya T, Tanji K, Ozaki T.
  • Journal Title: Journal of Clinical Biochemistry and Nutrition Volume: 56 Issue: 2 Pages: 91-97
  • DOI: 10.3164/jcbn.14-134
  • NAID: 130004879384
  • ISSN: 0912-0009, 1880-5086
  • Peer Reviewed / Open Access
• [Journal Article] Desferrioxamine, an iron chelator, inhibits CXCL10 expression induced by polyinosinic-polycytidylic acidin U373MG human astrocytoma cells. (2015)
  • Author(s): Imaizumi T, Sakashita N, Mushiga Y, Yoshida H, Hayakari R, Xing F, Wang L, Matsumiya T, Tanji K, Chiba Y, Furudate K, Kawaguchi S, Murakami M, Tanaka H.
  • Journal Title: Neurosci Res. Volume: in press Pages: 10-16
  • DOI: 10.1016/j.neures.2015.01.001
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Carnosic acid attenuates apoptosis induced by amyloid-β 1-42 or 1-43 in SH-SY5Y human neuroblastoma cells. (2014)
  • Author(s): Meng P, Yoshida H, Tanji K, Matsumiya T, Xing F, Hayakari R, Wang L, Tsuruga K, Tanaka H, Mimura J, Kosaka K, Itoh K, Takahashi I, Kawaguchi S, Imaizumi T.
  • Journal Title: Neurosci Res Volume: - Pages: 1-9
  • DOI: 10.1016/j.neures.2014.12.003
  • Peer Reviewed / Open Access
• [Journal Article] Toll-like receptor 3 signaling contributes to the expression of a neutrophil chemoattractant, CXCL1 in human mesangial cells. (2014)
  • Author(s): Imaizumi T, Aizawa T, Segawa C, Shimada M, Tsuruga K, Kawaguchi S, Matsumiya T, Yoshida H, Joh K, Tanaka H.
  • Journal Title: Clin Exp Nephrol. Volume: -
  • Peer Reviewed
• [Journal Article] Phosphorylation of serine 349 of p62 in Alzheimer’s disease brain (2014)
  • Author(s): Tanji K, Miki Y, Ozaki T, Maruyama A, Yoshida H, Mimura J, Matsumiya T, Mori F, Imaizumi T, Itoh K, Kakita A, Takahashi H, Wakabayashi K
  • Journal Title: Acta Neuropathol Comm Volume: 2 Issue: 1 Pages: 50-50
  • DOI: 10.1186/2051-5960-2-50
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] Cell differentiation modulates intracellular antiviral responses in neuronal cells (2014)
  • Author(s): Matsumiya T, Hayakari R, Xing F, Yoshida H, Imaizumi T.
  • Organizer: 12th International Congress of Neuroimmunology
  • Place of Presentation: Mainz, Germany
  • Year and Date: 2014-11-09 – 2014-11-13