| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Outline of Final Research Achievements |
There is growing evidence that non-thermal plasma (NTP) irradiation has a broad array of biological effects. Since NTP irradiation has been reported to selectively damage cancer cells, its application is believed to be useful for cancer therapy. The indirect NTP irradiation method using plasma-activated medium (PAM) is equally as effective to kill cancer cells as the direct NTP irradiation method. The anti-cancer effects of PAM have been shown to be attributed to reactive oxygen species (ROS) within it. Oxidative stress caused by ROS induces the liberation of zinc (Zn) from intracellular Zn stores and Zn-dependent cell death. Therefore, Zn is thought to function as a second messenger activated by oxidative stress. In this study, we have demonstrated that Zn released from intracellular sources serves as a key mediator of PAM-induced cell death. Our findings suggest that the conversion of PAM stimulus to a Zn signal is a critical step in the PAM-induced cell death process.
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