| Project/Area Number |
26460639
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Laboratory medicine
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| Research Institution | Tohoku University |
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Principal Investigator |
Hiroshi Tada 東北大学, 大学病院, 講師 (50436127)
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| Co-Investigator(Kenkyū-buntansha) |
権田 幸祐 東北大学, 医学系研究科, 教授 (80375435)
大内 憲明 東北大学, 医学系研究科, 教授 (90203710)
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| Research Collaborator |
Nemoto Noriko 東北大学, 医学系研究科, 大学院生
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 蛍光ナノ粒子 / ABCトランスポーター / 乳癌幹細胞 / 蛍光イメージング |
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| Outline of Final Research Achievements |
Assessing breast cancer tissues for expression of cancer stemness biomarkers, such as breast cancer resistance protein (BCRP) which is a member of ABC transporter, may provide critical information for patient prognosis. We developed a novel immunohistochemical method with new fluorescent nanoparticle, phosphor integrated dot (PID), by single-particle imaging using tissue samples from patients with clinical information. We successfully calculated the number of PID particles in a cell and defined it as nano patho score that reflects the level of BCRP protein expression in cancer cells. The BCRP nano patho score in 13 cases was correlated proportionally with the score of Ki67 as assessed by immunohistochemical method (R =0.62). But BCRP nano patho score were not correlated with breast cancer recurrence rate in breast cancer patients who were treated with preoperative chemotherapy
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