Development of lipoprotein lipase assays targeted to remnant lipoproteins in cardiovascular diseases.

• Project/Area Number: 26460640
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Laboratory medicine
• Research Institution: Kagawa Nutrition University (2016); Gunma University (2014-2015)
• Principal Investigator: Nakajima Katsuyuki 女子栄養大学, 付置研究所, 客員教授 (10444051)
• Research Collaborator: Okajima Fumikazu 群馬大学, 生体調節研究所, 教授 (30142748)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2014: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
• Keywords: リポ蛋白リパーゼ（LPL) / レムナントリポ蛋白 / 脂肪負荷 / 中性脂肪 / 肝性リパーゼ（HTGL) / 中性脂肪（TG) / 冠動脈硬化症 / レムナント受容体 / レムナント / リポプロテインリパーゼ（LPL） / アポC3 / tetrahydrolipstatin / LPL

Outline of Final Research Achievements

Plasma TG levels are known to increase after food intake. Also it has been believed that plasma LPL levels increase associated with the increase of plasma TG. However, we found that LPL levels don't change after fat load and sugar intake in non-heparin plasma.We also found that most of the increased TG after food intake was found in remnant lipoprotein fraction as RLP-TG.RLP-TG increased greatest in 4-6 h after food intake which was associated with the LPL concentration in remnant lipoproteins (LPL/RLP-TG interaction) .

Research Products

• [Journal Article] Lipoprotein lipase does not increase significantly in the postprandial plasma. (2017)
  • Author(s): Ishiyama N, Sakamaki K, Shimomura Y, Kotani K, Tsuzaki K, Sakane N, Miyashita K, Fukamachi I, Kobayashi J, Stanhope KL, Havel PJ, Kamachi K, Tanaka A, Tokita Y, Machida T, Murakami M, Nakajima K.
  • Journal Title: Clinica Chimica Acta Volume: 464 Pages: 204-210
  • DOI: 10.1016/j.cca.2016.11.035
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Triglyceride content in remnant lipoproteins is significantly increased after food intake and is associated with plasma lipoprotein lipase. (2017)
  • Author(s): Nakajima K, Tokita Y, Sakamaki K, Shimomura Y, Kobayashi J, Kamachi K, Tanaka A, Stanhope KL, Havel PJ, Wang T, Machida T, Murakami M.
  • Journal Title: Clinica Chimica Acta Volume: 465 Pages: 45-52
  • DOI: 10.1016/j.cca.2016.12.011
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] The majority of lipoprotein lipase in plasma is bound to remnant lipoproteins: A new definition of remnant lipoproteins. (2016)
  • Author(s): Sato K, Okajima F, Miyashita K, Imamura S, Kobayashi J, Stanhope KL, Havel PJ, Machida T, Sumino H, Murakami M, Schaefer E, Nakajima K.
  • Journal Title: Clin Chim Acta Volume: 461 Pages: 114-125
  • DOI: 10.1016/j.cca.2016.06.020
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Comparison of the effect of post-heparin and pre-heparin lipoprotein lipase and hepatic triglyceride lipase on remnant lipoprotein metabolism (2015)
  • Author(s): Shirakawa T, Nakajima K, Shimomura Y, Kobayashi J, Stanhope K, Havel P, Machida T, Sumino H, Murakami M
  • Journal Title: Clinica Chimica Acta Volume: 440 Pages: 193-200
  • DOI: 10.1016/j.cca.2014.07.020
  • Peer Reviewed / Open Access
• [Journal Article] The role of circulating lipoprotein lipase and adiponectin on the particle size of remnant lipoproteins in patients with diabetes mellitus and metabolic syndrome (2015)
  • Author(s): Shirakawa T, Nakajima K, Yatsuzuka SI, Shimomura Y, Kobayashi J, Machida T, Sumino H, Murakami M
  • Journal Title: Clinica Chimica Acta Volume: 440 Pages: 123-132
  • DOI: 10.1016/j.cca.2014.10.029
  • Peer Reviewed / Open Access
• [Journal Article] Relationship between carotid artery intima-media thickness and small dense low-density lipoprotein cholesterol concentrations measured by homogeneous assay in Japanese subjects (2015)
  • Author(s): Aoki T, Yagi H, Sumino H, Tsunekawa K, Araki O, Kimura T, Nara M, Ogiwara T, Nakajima K, Murakami M
  • Journal Title: Clin Chim Acta Volume: 442 Pages: 110-114
  • DOI: 10.1016/j.cca.2015.01.010
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Determination of serum lipoprotein lipase using a latex particle-enhanced turbidimetric immunoassay with an automated analyzer (2015)
  • Author(s): Machida T, Miyashita K, Sone T, Tanaka S, Nakajima K, Saito M, Stanhope K, Havel P, Sumino H, Murakami M
  • Journal Title: Clin Chim Acta Volume: 442 Pages: 130-135
  • DOI: 10.1016/j.cca.2015.01.016
  • Peer Reviewed / Open Access
• [Journal Article] Small dense LDL cholesterol measured by homogeneous assay in Japanese healthy controls, metabolic syndrome and diabetes patients with or without a fatty liver (2014)
  • Author(s): Kikkawa K, Nakajima K, Shimomura Y, Tokita Y, Machida T, Sumino H, Murakami M
  • Journal Title: Clin Chim Acta Volume: 438 Pages: 70-79
  • DOI: 10.1016/j.cca.2014.07.017
  • Peer Reviewed / Open Access
• [Presentation] Lipoprotein Lipase and Remnant Lipoprotein Formation (2016)
  • Author(s): Katsuyuki Nakajima
  • Organizer: 5th ISCD Meeting, November 9-10, 2016
  • Place of Presentation: Bosston, USA
  • Year and Date: 2016-11-09
  • Invited
• [Presentation] Significant increase of TG in remnant lipoproteins after food intake and its association with LPL concentration in plasma. (2016)
  • Author(s): Katsuyuki Nakajima 1, Yoshiharu Tokita 2, Koji Sakamaki3, Younosuke Shimomura3, Kimber L. Stanhope 3, Peter J. Havel 4, Tetsuo Machida 1, Masami Murakami 1
  • Organizer: 39th ELC Meeting
  • Place of Presentation: Tutzing, Germany
  • Year and Date: 2016-09-07
  • Int'l Joint Research
• [Presentation] Lipoprotein Lipase Does Not Increase in Postprandial Plasma. (2016)
  • Author(s): N. Ishiyama1, K. Nakajima1, K. Sakamaki 1, Shimomura1, K. Kotani2 , K. Tsuzaki3, N. Sakane3 K. Miyashit4, I. Fukamachi 4, J. Kobayashi5, Kimber L. Stanhope6, Peter J. Havel6 Y. Tokita7 , T. Machida7, M. Murakami7.
  • Organizer: 39th ELC Meeting
  • Place of Presentation: Tutzing, Germany
  • Year and Date: 2016-09-07
  • Int'l Joint Research
• [Presentation] 血中リポ蛋白リパーゼとレムナント・リポ蛋白の相互作用について (2015)
  • Author(s): 中嶋克行、佐藤幸市、岡島史和 他
  • Organizer: 第57回日本脂質生化学会
  • Place of Presentation: 東京都千代田区一ツ橋2-1-2 学術総合センター
  • Year and Date: 2015-05-28
• [Presentation] More than 80% of circulating lipoprotein lipase in serum (pre-heparin) is bound to remnant lipoproteins. (2014)
  • Author(s): Katsuyuki Nakajima, Kazuya Miyashita , Shigeyuki Imamura Junji Kobayashi , Takashi Shirakawa, Shinichi Yatsuzuka, Younosuke Shimomura, Tetsuo Machida , Hiroyuki Sumino , Masami Murakami.
  • Organizer: 82 th European Atherosclerosis Society Congress at Madrid, Spain on June 1-3, 2014
  • Place of Presentation: Madrid, Spain
  • Year and Date: 2014-06-01 – 2014-06-03