High-density lipoprotein HDL and apolipoprotein M as the glucose metabolism marker

• Project/Area Number: 26460641
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Laboratory medicine
• Research Institution: Gunma University
• Principal Investigator: Kimura Takao 群馬大学, 大学院医学系研究科, 講師 (90396656)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 経口血糖負荷試験 / 動脈硬化症 / 糖代謝 / 高密度リポ蛋白 / アポリポ蛋白 / 経口ブドウ糖負荷試験 / 糖尿病 / HDL / インスリン / アポリポタンパクM

Outline of Final Research Achievements

We assessed the oral glucose tolerance test (OGTT) and lipid metabolism among young and middle aged Japanese subjects. We also measured the serum concentration of apolipoprotein M (ApoM) using ELISA. Serum ApoM concentrations were positively correlated with total cholesterol (TC) and high-density lipoprotein cholesterol (HDL-C). Serum ApoM concentrations were positively correlated with Matsuda Index which represents both hepatic and peripheral tissue sensitivity to insulin. Matsuda Index is simple to calculate and provides a reasonable approximation of whole-body insulin sensitivity from the OGTT. OGTT needs four times of drawing blood. Therefore to calculate Matsuda Index needs four times of drawing blood. The positive correlation between serum ApoM concentration and Matsuda Index indicates that serum ApoM concentration might reflect whole-body insulin sensitivity. In summary, whole-body insulin sensitivity might be assessed by measurement of serum ApoM concentration.

Research Products

• [Journal Article] Fasting serum insulin levels and insulin resistance are associated with blood rheology in Japanese young adults without diabetes. (2016)
  • Author(s): Yoshida K, Kimura T, Aoki T, Tsunekawa K, Araki O, Shoho Y, Nara M, Sumino H, Murakami M.
  • Journal Title: J Int Med Res Volume: 44 Issue: 3 Pages: 496-507
  • DOI: 10.1177/0300060515627561
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Biological Antioxidant Potential Negatively Correlates With Carotid Artery Intima-Media Thickness (2016)
  • Author(s): Yagi H, Sumino H, Yoshida K, Aoki T, Tsunekawa K, Araki O, Kimura T, Nara M, Nakajima K, Murakami M
  • Journal Title: International Heart Journal Volume: 57 Issue: 2 Pages: 220-225
  • DOI: 10.1536/ihj.15-389
  • NAID: 130005139772
  • ISSN: 1349-2365, 1349-3299
  • Peer Reviewed / Open Access
• [Journal Article] Impaired blood rheology is associated with endothelial dysfunction in patients with coronary risk factors. (2015)
  • Author(s): Yagi H, Sumino H, Aoki T, Tsunekawa K, Araki O, Kimura T, Nara M, Ogiwara T, Murakami M.
  • Journal Title: Clin Hemorheol Microcirc. Volume: 62 Issue: 2 Pages: 139-150
  • DOI: 10.3233/ch-151960
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Type 2 Iodothyronine Deiodinase Activity Is Required for Rapid Stimulation of PI3K by Thyroxine in Human Umbilical Vein Endothelial Cells. (2015)
  • Author(s): Aoki T, Tsunekawa K, Araki O, Ogiwara T, Nara M, Sumino H, Kimura T, Murakami M.
  • Journal Title: Endocrinology Volume: 156 Issue: 11 Pages: 4312-4324
  • DOI: 10.1210/en.2014-1988
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Relationship between carotid artery intima-media thickness and small dense low-density lipoprotein cholesterol concentrations measured by homogeneous assay in Japanese subjects (2015)
  • Author(s): Aoki T, Yagi H, Sumino H, Tsunekawa K, Araki O, Kimura T, Nara M, Ogiwara T, Nakajima K, Murakami M
  • Journal Title: Clin Chim Acta Volume: 442 Pages: 110-114
  • DOI: 10.1016/j.cca.2015.01.010
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] Assessment of the glucose tolerance by the shape of plasma glucose concentration curve during OGTT among young Japanese subjects (2016)
  • Author(s): Tsunekawa K, Aoki T, Araki O, Ogiwara T, Kimura T, Nara M, Sumino H, Machida T, Murakami M
  • Organizer: The 32nd World Congress of the International Federation of Biomedical Laboratory Science
  • Place of Presentation: 神戸
  • Year and Date: 2016-08-31
  • Int'l Joint Research
• [Presentation] 群馬大学医学部附属病院における検査部外来の現状 (2015)
  • Author(s): 木村孝穂、常川勝彦、青木智之、角野博之、荒木修、奈良誠人、小磯博美、徳江豊、 村上正巳
  • Organizer: 第62回日本臨床検査医学会学術集会
  • Place of Presentation: 長良川国際会議場
  • Year and Date: 2015-11-22
• [Presentation] メタボリックシンドロームにおける血中アポリポ蛋白M濃度の検討 (2014)
  • Author(s): 関口琢磨，常川勝彦，木村孝穂，正保佳史，青木智之，荒木修，角野博之，荻原貴之，奈良誠人，町田哲男,村上正巳
  • Organizer: 第61回日本臨床検査医学会学術集会
  • Place of Presentation: 福岡国際会議場・福岡
  • Year and Date: 2014-11-25
• [Presentation] 血清中アポリポ蛋白M濃度測定の意義：アポリポ蛋白Mと凝固線溶系マーカーの相関の解析 (2014)
  • Author(s): 木村孝穂，青木智之，清水知，宮崎修，深町勇，角野博之、中嶋克行，岡島史和，村上正巳
  • Organizer: 第46回日本動脈硬化学会総会・学術集会
  • Place of Presentation: 京王プラザホテル・東京
  • Year and Date: 2014-07-11