Development of diagnostic test and analysis of pathogenesis for Johne's disease

• Project/Area Number: 26460650
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Laboratory medicine
• Research Institution: Tottori University
• Principal Investigator: MATSUBA Takashi 鳥取大学, 医学部, 講師 (20304206)
• Research Collaborator: MORI Yasuyuki 農研機構, 動物衛生研究部門 ; KAWAJI Satoko 農研機構, 動物衛生研究部門 ; NAGATA Reiko 農研機構, 動物衛生研究部門
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2014: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 細菌感染症 / 診断 / 血清反応 / 組換えタンパク / 抗原 / ELISA / 抗体 / 感染症 / 細菌

Outline of Final Research Achievements

New antigen for serodiagnosis of bacterial infectious disease causing chronic enteritis in domestic animals was bioinfomatically searched and then screened with an immune serum. A remarkable reactivity to each purified recombinant protein was confirmed. By sera obtained from two experimentally infected cattle, the increased reactivities corresponding to the detection of the bacterial gene from the feces and the culture positivity was observed in each recombinant protein. Furthermore, antibody class dependent reactivity depending on the infected individuals was observed. Our results suggest the possibility of pathological changes depending on host immune responsiveness.

Research Products

• [Journal Article] Evaluation of in-house loop-mediated isothermal amplification for tuberculosis diagnosis in comparison with Xpert MTB/RIF. (2017)
  • Author(s): Habeenzu C, Nakajima C, Solo E, Bwalya P, Kajino, K, Miller M, Kurosawa Y, Mudenda V, Kasonka L, Suzuki Y, Matsuba T
  • Journal Title: J. Infect. Dev. Ctries. Volume: 印刷中
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Difference in antibody rsponses to Mycobacterium tuberculosis antigens in Japanese tuberculosis patients infected with the Beijing/non-Beijing genotype. (2017)
  • Author(s): Zhao J, Shiratori B, Okumura M, Yanai H, Matsumoto M, Nakajima C, Mizuno K, Ono K, Oda T, Chagan-Yasutan H, Ashino Y, Matsuba T, Yoshiyama T, Suzuki Y, Hattori T
  • Journal Title: J. Immunol. Res. Volume: 2017 Pages: 1-12
  • DOI: 10.1155/2017/4797856
  • Peer Reviewed / Open Access
• [Journal Article] Antigenic characterisation of dimorphic surface protein in Mycobacterium tuberculosis. (2016)
  • Author(s): Matsuba T, Siddiqi UR, 3, Hattori T, Nakajima C, Fujii J, Suzuki Y
  • Journal Title: FEMS Microbiol. Lett. Volume: 印刷中 Issue: 10 Pages: 1-8
  • DOI: 10.1093/femsle/fnw082
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Development and evaluation of an interferon-γ release assay in Asian elephants (<i>Elephas maximus</i>) (2016)
  • Author(s): Paudel S, Villanueva MA, Mikota SK, Nakajima C, Gairhe KP, Subedi S, Rayamajhi N, Sashika M, Shimozuru M, Matsuba T, Suzuki Y, Tsubota T
  • Journal Title: Journal of Veterinary Medical Science Volume: 78 Issue: 7 Pages: 1117-1121
  • DOI: 10.1292/jvms.15-0701
  • NAID: 130005253820
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed / Int'l Joint Research
• [Presentation] ヨーネ菌組換え抗原タンパクに対する実験感染牛の血清抗体反応性 (2017)
  • Author(s): 松葉隆司，永田礼子，川治聡子，中島千絵，鈴木定彦，藤井潤
  • Organizer: 第90回日本細菌学会総会
  • Place of Presentation: 仙台国際センター（宮城県・仙台市）
  • Year and Date: 2017-03-19
• [Presentation] ヨーネ菌特異抗原の探索と 組換えタンパクへの血清反応性 (2016)
  • Author(s): 松葉隆司，永田礼子，川治聡子
  • Organizer: 第47回結核・非定型抗酸菌症治療研究会
  • Place of Presentation: 笹川会館（東京都・港区）
  • Year and Date: 2016-12-04
• [Presentation] 一般家庭浴室のマイコバクテリア (2014)
  • Author(s): 松葉隆司、中島千絵、藤井潤、鈴木定彦
  • Organizer: 第45回結核・非定型抗酸菌治療研究会
  • Place of Presentation: 笹川記念会館（東京）
  • Year and Date: 2014-12-08
• [Book] 非結核性抗酸菌の基礎と臨床, 斉藤肇編 (2015)
  • Author(s): 鈴木定彦、中島千絵、福島由華里、田丸亜貴、松葉隆司
  • Publisher: 医薬ジャーナル社