| Project/Area Number |
26460655
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Laboratory medicine
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| Research Institution | Juntendo University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
堀本 義哉 順天堂大学, 医学部, 准教授 (40424246)
北野 滋久 国立研究開発法人国立がん研究センター, 早期・探索臨床研究センター, 医員 (60402682)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | 乳癌 / 乳腺髄様癌 / 腫瘍浸潤リンパ球 / トリプルネガティブ乳癌 / リンパ球 |
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| Outline of Final Research Achievements |
On first year, we could not get fresh frozen specimen, because of its rare special type of breast cancer. On our second year, we evaluated lymphocyte infiltrate pattern of conventional invasive ductal carcinoma as control group. In last year, we focus triple-negative breast cancer (negative hormon (estrogen and progesterone) receptor and HER2 negative), which is the worst prognostic group and have no therapeutic choice. We picked up two groups; much of tumor infiltrate lymphocytes and no lymphocytic infiltrate group in triple-negative breast cancer, searched by pathologist on HE sections only. DNA extraction and purifying by using QIAGEN FFPE tissue kit quality control and amplified by Real-time PCR. We tested gene mutation specifying lymphocytic infiltrate breast cancer by comprehensive mutation analysis.
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