Development of assay utilizing DNA aptamer and applying to a clinical examination

• Project/Area Number: 26460656
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Laboratory medicine
• Research Institution: Showa University
• Principal Investigator: Sano Yoshihiro 昭和大学, 薬学部, 准教授 (40338538)
• Co-Investigator(Kenkyū-buntansha): 荒川 秀俊 昭和大学, 薬学部, 教授 (70129807)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: アプタマー / オキシトシン / アスペルガー / 臨床分析化学 / ASD / ペプチドホルモン / 臨床検査 / 自閉症 / ホルモン

Outline of Final Research Achievements

We studied to development assay utilizing aptamer for oxytocin (OXT), was shown to play important key roles in social interactions and social behavior and a series of studies investigated the relationship between autism spectrum disorder (ASD) and OXT, and applying to a clinical examination by using that assay.
As a result of SELEX, the aptamers (ssDNA) for OXT prepared in random ssDNA BANK and sequence of those were high homologous sequence. Therefore, we suggested that aptamer for OXT was selected from many ssDNA containing random sequence. Moreover, we made a measurement of OXT in Japanese human plasma on this study by using immunoassay for OXT. As a result, the average levels of males and females were 30 and 25 pg/mL respectively, here.

Research Products

• [Journal Article] Development of a highly specific enzyme immunoassay for oxytocin and its use in plasma samples. (2016)
  • Author(s): Shiomi Haraya, Koji Karasawa,Yoshihiro Sano, Kimiko Ozawa, Nobumasa Kato and Hidetoshi Arakawa,
  • Journal Title: Annals of Clinical Biochemistry, 54(1):101-106, 2016 Volume: 54 Pages: 101-106
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] A cohort study of the level of plasma oxytocin associated with autism spectrum disorder in Japanese males, females, and pregnant females. (2016)
  • Author(s): Yoshihiro Sano, Noriko Watanabe, Emiko Suzuki, Kazuhisa Shimodaira, Nobumasa Kato, and Hidetoshi Arakawa,
  • Journal Title: Clinical & Medical Biochemistry, March 2, 2016 Volume: 2 Pages: 0-0
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Presentation] Novel assay for oxytocin using bioluminescence enzyme immunoassay (2016)
  • Author(s): Koji Karasawa, Yoshihiro Sano, Hidetoshi Arakawa
  • Organizer: 68th AACC Annual Scientific Meeting & Clinical Lab Expo, Aug. 2016, Philadelphia (USA)
  • Place of Presentation: Philadelphia (USA)
  • Year and Date: 2016-07-31
  • Int'l Joint Research
• [Presentation] 生物発光酵素イムノアッセイを用いた血中オキシトシン測定法の開発 (2016)
  • Author(s): 唐沢浩二、佐野佳弘、大熊博、荒川秀俊
  • Organizer: 日本薬学会第136年会
  • Place of Presentation: 神奈川
  • Year and Date: 2016-03-26
• [Presentation] Development of a highly sensitive enzyme immunoassay for oxytoci (2015)
  • Author(s): H. Arakawa, S. Haraya, K. Karasawa, Y. Sano, H. Ohkuma
  • Organizer: the 2015 AACC Annual Meeting & Clinical Lab Expo
  • Place of Presentation: アトランタ、アメリカ
  • Year and Date: 2015-07-27
• [Presentation] ヒト血中Oxytocin測定とその現状 (2014)
  • Author(s): 佐野佳弘、原谷汐美、渡辺倫子、鈴木恵美子、小澤仁子、下平和久、加藤進昌、荒川秀俊
  • Organizer: バイオメディカル分析科学シンポジウム2014
  • Place of Presentation: 東京
  • Year and Date: 2014-08-20 – 2014-08-21
• [Book] 免疫測定法 －基礎から先端まで－ (2014)
  • Author(s): 小林典裕、荒川秀俊、佐野佳弘 分担著書
  • Total Pages: 325
  • Publisher: 講談社