| Project/Area Number |
26460662
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Laboratory medicine
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| Research Institution | Kansai Medical University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
小宮山 豊 関西医科大学, 医学部, 講師 (40140264)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Keywords | 内因性ジギタリス / 質量分析イムノアッセイ / 高血圧 / 生理活性物質 / 質量分析 / マリノブファギン / マリノブフォトキシン / テロシノブフォトキシン / ELISA / レニン / アルドステロン |
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| Outline of Final Research Achievements |
This research is a part of patho-physiological study of endogenous digitalis-like factors (EDLFs). We have marinobufagin, telocinobufagin, and their related compounds, maronobufotoxin and telicinobufotoxin. Moreover, we reported they are existed in human plasma. EDLFs have low monocular compounds with steroid ring. Therefore their analytic methods have only some techniques. Recently MISA, which has been interested by same field’s researchers, can be a technique for high sensitive qualitative as well as quantitative analysis in lesser sample volunme. We tried MISA was used more easily and high specific pretreatment of our ELISA instead of C18 seppak columns. Our each antibodies was absorbed in MISA tubes, and then samples was added in MISA tube. In this tube, we tried to extract only target substances.
Unfortunately, the adequate antigen‐antibody reactionin was not achieved in this study.
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