| Project/Area Number |
26460670
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Laboratory medicine
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| Research Institution | Kanazawa University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
寳達 明彦 金沢大学, 医学系, 協力研究員 (00623662)
藤野 陽 金沢大学, 保健学系, 准教授 (40361993)
清水 渉 日本医科大学, 大学院医学研究科, 大学院教授 (50399606)
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| Co-Investigator(Renkei-kenkyūsha) |
SEKINE Akihiro 京都大学, 医学系研究科, 教授 (30425631)
AIBA Takeshi 独立行政法人国立循環器病研究センター, 循環器内科, 医長 (40574348)
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| Research Collaborator |
TSUDA Toyonobu
TANAKA Yoshihiro
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | ゼブラフィッシュ / 次世代シークエンス / モルフォリノ / Crispr/Cas9システム / 遺伝子改変ゼブラフィッシュ / 遺伝性不整脈 / 全エクソーム解析 / 次世代シークエンサー / 機能解析 |
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| Outline of Final Research Achievements |
We performed genetic analysis using next generation sequencing for patients with inherited arrhythmia.We performed electrophysiological study for detected gene mutations in ion channel genes using patch clamp methods. We evaluated mutations in KCNH2 gene using zebrafish embryo. Knockdown or CRISPR-mediated deletions of the human LMNA ortholog, lmna in zebrafish recapitulated the electrophysiological phenotypes of human early-onset bradycardia.
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