Diagnostic markers of glandular lesion and their precursor lesions of uterine cervix

• Project/Area Number: 26460673
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Laboratory medicine
• Research Institution: Shinshu University
• Principal Investigator: OTA Hiroyoshi 信州大学, 学術研究院保健学系, 教授 (50273107)
• Research Collaborator: ASAKA Shiho ; KUBOTA Seiko
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 胃型腺癌 / 子宮頸部腺系病変 / TFF / 子宮頸部腺癌 / 分葉状子宮頸部腺過形成 / LEGH / 子宮頸部分葉状頸管腺過形成

Outline of Final Research Achievements

In lobular endocervical glandular hyperplasia (LEGH), adenocarcinoma in situ and invasive adenocarcinoma, TFF1 was expressed 100/75/82.5%, TFF2 was expressed 100/37.5/44.8% and GlcNAcα→R recognized by antibody HIK1083 was expressed 100/37.5/44.8%. TFF2 expressed the same localizations with those of GlcNAcα→R. The secretions within the glandular lumens reacted positively for TFF2. TFF2 levels in cervical discharge measured using ELISA were higher in patients with higher scores of latex agglutination test using HIK1083 antibody. Screening of TFFs in cervical discharge may facilitate detection of cervical glandular lesion.

Research Products

• [Presentation] TFF2 is a useful immunohistochemical marker for gastric type glandular lesion of uterine cervix (2017)
  • Author(s): Shiho Asaka, Nozomi Yazaki, Masayuki Ito, Chinatsu Kobayashi, Tomoyuki Nakajima, Yukihiro kobayashi, Koichi Ida, Keiko Ishi, Takeshi Uehara, Takayuki Honda, and Hiroyoshi Ota
  • Organizer: 第106 回日本病理学会総会
  • Place of Presentation: 京王プラザホテル，東京
  • Year and Date: 2017-04-27
• [Presentation] Trefoil Factor Family 2 Protein: A Promising Immunohistochemical Marker for Diagnosing Lobular Endocervical Glandular Hyperplasia and Gastric-type Adenocarcinoma of Uterine Cervix (2017)
  • Author(s): Shiho Asaka, Tomoyuki Nakajima, and Hiroyoshi Ota
  • Organizer: USCAP2017
  • Place of Presentation: Henry B. Gonzalez Convention Center, San Antonio, TX, USA
  • Year and Date: 2017-03-04
  • Int'l Joint Research
• [Presentation] 子宮頸部胃型腺系病変における胃粘液上皮マーカーの有用性の検討 (2016)
  • Author(s): 浅香 志穂，中嶋 智之，太田浩良
  • Organizer: 第105回日本病理学会総会
  • Place of Presentation: 仙台国際センター（仙台）
  • Year and Date: 2016-05-13
• [Presentation] TFF1の免疫染色による子宮頸部腺系病変における胃型病変の検出とその有用性について Detection of gastric type‐cervical glandular lesions by immunostaining for TFF1 and its significance (2016)
  • Author(s): 浅香志穂，中嶋智之，久郷佳央梨，柏木里沙，仲田梨恵，小林幸弘，上原剛，石井恵子，太田浩良
  • Organizer: 第105 回日本病理学会総会
  • Place of Presentation: 仙台国際センター ，仙台
  • Year and Date: 2016-05-12