Stress response of cancer cells focusing the sphingolipid metabolism and its modulation by food ingredients
Project/Area Number |
26460674
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Laboratory medicine
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Research Institution | Chubu University (2015-2016) Nagoya University (2014) |
Principal Investigator |
MURATE Takashi 中部大学, 生命健康科学部, 教授 (30239537)
|
Co-Investigator(Kenkyū-buntansha) |
高木 明 名古屋大学, 医学系研究科(保健), 准教授 (30135371)
小嶋 哲人 名古屋大学, 医学系研究科(保健), 教授 (40161913)
鈴木 元 名古屋大学, 医学系研究科, 准教授 (80236017)
|
Project Period (FY) |
2014-04-01 – 2017-03-31
|
Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
|
Keywords | Sphingolipid metabolism / Cancer cell / Stress response / Food ingredient / Phytochemical / Anti-cancer action / レスベラトロール / 酸性スフィンゴミエリナーゼ / 転写調節 / 白血病細胞株 / 悪性腫瘍細胞株 / スフィンゴ脂質代謝 / 代謝酵素遺伝子発現調節 / SPHK2 / 血清除去 / ACDase / 前立腺癌細胞株 / アンドロジェン / USP2 / hypoxia / serum-depleted condition / CREB / transcription |
Outline of Final Research Achievements |
We have published 9 papers corresponding to the main title of the grant No. 26460674 between 2013 and 2016. Among them, 6 papers described the stress responses and the sphingolipid metabolism of cancer cells, whereas 2 papers analyzed the anti-cancer drug resistance of malignant cells. One paper elucidated the molecular mechanism of anti-cancer function of resveratrol, a food ingredient. Based on these works, we concluded that malignant cells utilize heterogeneous mechanisms including aberrant sphingolipid metabolism, changes of DNA repair system, microtubules dynamics, mRNA half-life, and chromatin remodeling. As far as the sphingolipid metabolism was concerned, our data strongly supported the validity of the sphingolipid rheostat model telling that the balance between ceramide and sphingosine 1-phospahte determined cells' fate such as cell growth, survival, motion and differentiation.
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Report
(4 results)
Research Products
(13 results)
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[Journal Article] Resveratrol-induced transcriptional up-regulation of ASMase (SMPD1) of human leukemia and cancer cells.2016
Author(s)
Mizutani N, Omori Y, Kawamoto Y, Sobue S, Ichihara M, Suzuki M, Kyogashima M, Nakamura M, Tamiya-Koizumi K, Nozawa Y, Murate T.
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Journal Title
Biochem Biophys Res Commun
Volume: 470
Issue: 4
Pages: 851-856
DOI
Related Report
Peer Reviewed
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[Journal Article] C1q/TNF-related protein-1 functions to protect against acute ischemic injury in the heart2016
Author(s)
Yuasa D.,Ohashi K.,Shibata R.,Mizutani N.,Kataoka Y.,Kambara T.,Uemura Y.,Matsuo K.,Kanemura N.,Hayakawa S.,Hiramatsu-Ito M.,Ito M.,Ogawa H.,Murate T.,Murohara T. and Ouchi N.
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Journal Title
FASEB J
Volume: 30
Issue: 3
Pages: 1065-1075
DOI
Related Report
Peer Reviewed
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[Journal Article] Increased SPHK2 transcription of human colon cancer cells in serum-depleted culture: the involvement of CREB transcription factor.2015
Author(s)
Mizutani N, Omori Y, Tanaka K, Ito H, Takagi A, Kojima T, Nakatochi M, Ogiso H, Kawamoto Y, Nakamura M, Suzuki M, Kyogashima M, Koizumi K, Nozawa Y, Murate T.
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Journal Title
J Cell Biochem
Volume: 116
Issue: 10
Pages: 2227-2238
DOI
Related Report
Peer Reviewed
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[Journal Article] Increased Acid Ceramidase Expression depends on Upregulation of Androgen-dependent Deubiquitinases, USP2, in a Human Prostate Cancer Cell Line, LNCaP.2015
Author(s)
Naoki Mizutani, Minami Inoue, Yukari Omori, Hiromi Ito, Keiko Tamiya-Koizumi, Akira Takagi, Tetsuhito Kojima, Mitsuhiro Nakamura, Soichiro Iwaki, Masahiro Nakatochi, Motoshi Suzuki, Yoshinori Nozawa, Takashi Murate
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Journal Title
J. Biochem.
Volume: 印刷中
Issue: 4
Pages: 309-319
DOI
Related Report
Peer Reviewed
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[Journal Article] Phosphorylated Sp1 is the regulator of DNA-PKcs and DNA ligase IV transcription of daunorubicin-resistant leukemia cell lines2014
Author(s)
Nishida Y, Mizutani N, Inoue M, Omori Y, Tamiya-Koizumi K, Takagi A, Kojima T, Suzuki M, Nozawa Y, Minami Y, Ohnishi K, Naoe T, Murate T.
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Journal Title
Biochim Biophys Acta
Volume: 1839
Pages: 265-274
Related Report
Peer Reviewed / Acknowledgement Compliant
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