| Project/Area Number |
26460678
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Laboratory medicine
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| Research Institution | Yamaguchi University |
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Principal Investigator |
TSUNEOKA Hidehiro 山口大学, 医学(系)研究科(研究院), 教授 (40437629)
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| Co-Investigator(Kenkyū-buntansha) |
大津山 賢一郎 山口大学, 医学(系)研究科(研究院), 助教 (10432741)
柳原 正志 山口大学, 医学(系)研究科(研究院), 助教 (40379954)
徳田 信子 山口大学, 医学(系)研究科(研究院), 教授 (70227578)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | Bartonella henselae / 猫ひっかき病 / ELISA / CHAPS / サルコシン / CSD / N-ラウロイル-サルコシン |
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| Outline of Final Research Achievements |
The measurement of anti-Bartonella henselae IgG by highly-sensitive ELISA requires highly-specific purification of antigen. To obtain this antigen, it is needed 1) to culture Bartonella henserae in double-phase culture media (Heart infusion agar medium with 5％rabbit blood and D-MEM liquid medium), 2) to put the liquid in 0.1％ CHAPS (ampholytic surfactant) at 37℃ to extract insoluble components, which is to be put in 0.5% CHAPS for the second time to extract soluble components, and 3) to purify the protein in the soluble components using 245-310mMNaCL by DEAE-Sepharose chromatography. This purified solution contains highly-specific protein components and therefore, clinical implication of this protein as an antigen is greatly expected in the measurement of anti-Bartonella henserae IgG by ELISA.
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