Analysis of pain disease model-associated mitochondrial abnormalities in primary sensory neurons
| Project/Area Number |
26460706
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Pain science
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| Research Institution | Keio University |
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Principal Investigator |
Shibata Mamoru 慶應義塾大学, 医学部(信濃町), 講師 (60286466)
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| Co-Investigator(Kenkyū-buntansha) |
清水 利彦 慶應義塾大学, 医学部(信濃町), 講師 (40265799)
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| Project Period (FY) |
2014-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
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| Keywords | ミトコンドリア / 三叉神経節ニューロン / 片頭痛 / オートファジー / ミトファジー / 皮質拡延性抑制 / TRPV1 / カプサイシン / 神経修復機構 / 三叉神経節 / リソソーム / アナンダマイド / 神経障害性疼痛 / HMGB1 / ミクログリア / Toll-like receptor / 侵害性刺激 |
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| Outline of Final Research Achievements |
TRPV1 is activated by capsaicin. We applied 10 mM capsaicin-immersed cotton to the whisker pad for up to 6 days. Electron microscopic analysis revealed that there was extensive destruction of mitochondrial internal structure in small-sized trigeminal ganglion (TG) neurons at Day 4. Nevertheless, no discernible structural alterations were not observed in the small-sized TG neurons at Day 6, implying that there was a restorative process that normalized mitochondrial status in TG neurons exposed to repetitive TRPV1 stimulation. We established a PC12 clone stably expressing TRPV1. When exposed to capsaicin, these cells exhibited mitochondrial structural derangements in a dose-dependent manner. There were significantly increased mitophagy-affected mitochondria in capsaicin-treated cells. Our-cell-based assays imply that mitophagy plays an important role in the reparative process of mitochondrial health in capsaicin-treated cells.
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Report
(5 results)
Research Products
(30 results)
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[Presentation] Differential cellular localization of antioxidant enzymes in the trigeminal ganglion.2014
Author(s)
Shibata M, Sato H, Shimizu T, Shibata S, Toriumi H, Ebine T, Kuroi T, Iwashita T, Funakubo M, Kayama Y, Akazawa C, Wajima K, Nakagawa T, Okano H, Suzuki N.
Organizer
Pan-Asian/Pan-American Program, American Headache Society 2014, June 2014, Los Angeles.
Place of Presentation
Hyatt Regency Century Plaza, Los Angeles, USA
Year and Date
2014-06-26
Related Report
Invited
