Alpha2 delta-1 mediated synaptic plasticity in the dorsal horn of peripheral nerve injury model rats

• Project/Area Number: 26460712
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Pain science
• Research Institution: Hyogo Medical University
• Principal Investigator: Yamanaka Hiroki 兵庫医科大学, 医学部, 講師 (20340995)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 神経傷害性疼痛 / 脊髄後角 / シナプス / 可塑性 / 接着因子 / シナプス可塑性 / 細胞間接着因子 / カルシウムチャネル

Outline of Final Research Achievements

The present study demonstrated the following new findings in the neuropathic pain model rats:1) nerve injury increased alpha2 delta-1 and decreased phosphorylated L1-CAM in injured DRG neuron. 2) Alpha2 delta-1 showed co-localization with phosho-L1-CAM in the dorsal horn. 3) Nerve injury increased the attachment of synaptophysin-ir with alpha2 delta-1and phospho-L1-CAM ir varicosity. 4) Administration of pregabalin decreased Alpha3 delta-1, L1-CAM and phosphp-L1-CAM in the dorsal horn after nerve injury. 5) Administration of pregabalin inhibited the formation of synaptohpysin-L1-CAM-ir contact in the dorsal horn of neuropathic pain model rats.