Analysis of digestive organ cancer risk-associated genetic variations by using semiconductor-based next-gen sequencings

• Project/Area Number: 26460757
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Epidemiology and preventive medicine
• Research Institution: Chiba Cancer Center (Research Institute)
• Principal Investigator: MIKAMI Haruo 千葉県がんセンター(研究所), がん予防センター, 部長 (10332355)
• Co-Investigator(Renkei-kenkyūsha): NAGASE Hiroki 千葉県がんセンター(研究所), 所長 (90322073)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 癌 / 遺伝学 / 遺伝子 / ゲノム / 人間生活環境

Outline of Final Research Achievements

We conducted the next-gen sequencing approach to identify risk-associated genetic variants primarily in a cancer risk population. In cohort sample, 40 stomach cancer cases and 43 colon cancer cases were identified and considered as a cancer risk group, while 33 healthy individuals who did not develop any cancer by 70 years old were used as a low-risk control. The study revealed to identify more frequent unknown / non-synonymous germ-line mutations including non-sense variants in the risk group. We have identified potential functional non-synonymous germ-line mutations in 2 genes for risk of stomach cancer, 3 genes for colon cancer.
Cancer risk markers identified in this study can be useful to apply prevention, health check-up invitation, early-diagnosis for cancer in a regional population. Further analyses are necessary to demonstrate mechanisms involved in the cancer risk, which may be contributed by polymorphic germ-line alleles identified in this study.

Research Products

• [Journal Article] Associations between Dietary Patterns, ADRβ2 Gln27Glu and ADRβ3 Trp64Arg with Regard to Serum Triglyceride Levels: J-MICC Study. (2016)
  • Author(s): Nanri H, Nishida Y, Nakamura K, Tanaka K, Naito M, Yin G, Hamajima N, Takashima N, Suzuki S, Nindita Y, Kohno M, Uemura H, Koyama T, Hosono S, Mikami H, Kubo M, Tanaka H.
  • Journal Title: Nutrients Volume: 8 Issue: 9 Pages: 545-545
  • DOI: 10.3390/nu8090545
  • NAID: 120007097697
  • Peer Reviewed / Open Access
• [Journal Article] "Influence of cigarette smoking and inflammatory gene polymorphisms on glycated hemoglobin in the Japanese general population" (2016)
  • Author(s): Nishida Y, Hara M, Sakamoto T, Shinchi K, Kawai S, Naito M, Hamajima N, Kadota A, Suzuki S, Ibusuki R, Hirata A, Yamaguchi M, Kuriyama N, Oze I, Mikami H, Kubo M, Tanaka H.
  • Journal Title: Prev. Med. Rep. Volume: 3 Pages: 288-295
  • DOI: 10.1016/j.pmedr.2016.03.010
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] ALDH2 polymorphism is associated with fasting blood glucose through alcohol consumption in Japanese men. (2016)
  • Author(s): Yin G, Naito M, Wakai K, Morita E, Kawai S, Hamajima N, Suzuki S, Kita Y, Takezaki T, Tanaka K, Morita M, Uemura H, Ozaki E, Hosono S, Mikami H, Kubo M, Tanaka H; Japan Multi-institutional Collaborative Cohort (J-MICC) Study Group.
  • Journal Title: Nagoya J Med Sci Volume: 78 Pages: 183-193
  • NAID: 120005756085
  • Peer Reviewed / Open Access
• [Journal Article] Polymorphisms in CYP19A1, HSD17B1 and HSD17B2 genes and serum sex hormone level among postmenopausal Japanese women. Japan Multi-Institutional Collaborative Cohort (J-MICC) Study Group. (2015)
  • Author(s): Hosono S, Ito H, Oze I, Higaki Y, Morita E, Takashima N, Suzuki S, Shimatani K, Mikami H, Ohnaka K, Ozaki E. Katsuura-Kamano S, Kubo M, Nagata C, Naito M, Hamajima N, Tanaka H.
  • Journal Title: Maturitas Volume: 62 Issue: 4 Pages: 394-401
  • DOI: 10.1016/j.maturitas.2015.08.003
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] A variant of the CLOCK gene and related haplotypes are associated with the prevalence of type 2 diabetes in the Japanese population. Japan Multi-institutional Collaborative Cohort (J-MICC) Study Group. (2015)
  • Author(s): Uemura H, Katsuura-Kamano S, Yamaguchi M, Arisawa K, Hamajima N, Hishida A, Kawai S, Oze I, Shinchi K, Takashima N, Suzuki S, Nakahata N, Mikami H, Ohnaka K, Kuriyama N, Kubo M, Tanaka H.
  • Journal Title: J Diabetes Volume: online Issue: 5 Pages: 667-676
  • DOI: 10.1111/1753-0407.12344
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Association between brain-muscle-ARNT-like protein-2 (BMAL2) gene polymorphism and type 2 diabetes mellitus in obese Japanese individuals: A cross-sectional analysis of the Japan Multi-institutional Collaborative Cohort Study. (2015)
  • Author(s): Yamaguchi M, Uemura H, Arisawa K, Katsuura-Kamano S, Hamajima N, Hishida A, Suma S, Oze I, Nakamura K, Takashima N, Suzuki S, Ibusuki R, Mikami H, Ohnaka K, Kuriyama N, Kubo M, Tanaka H.
  • Journal Title: Diabetes Res. Clin. Pract. Volume: 110 Issue: 3 Pages: 301-308
  • DOI: 10.1016/j.diabres.2015.10.009
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Associations between polymorphisms of interleukin-6 and related cytokine genes and serum liver damage markers: a cross-sectional study in the Japan Multi-Institutional Collaborative Cohort (J-MICC) Study. (2015)
  • Author(s): Sugimoto Y, Wakai K, Nakagawa H, Suma S, Sasakabe T, Sakamoto T, Takashima N, Suzuki S, Ogawa S, Ohnaka K, Kuriyama N, Arisawa K, Mikami H, Kubo M, Hosono S, Hamajima N, Tanaka H; J-MICC Study Group.
  • Journal Title: Gene. Volume: 557 Issue: 2 Pages: 158-162
  • DOI: 10.1016/j.gene.2014.12.025
  • Peer Reviewed
• [Journal Article] GCK, GCKR polymorphisms and risk of chronic kidney disease in Japanese individuals: data from the J-MICC Study. (2014)
  • Author(s): Hishida A, Takashima N, Turin TC, Kawai S, Wakai K, Hamajima N, Hosono S, Nishida Y, Suzuki S, Nakahata N, Mikami H, Ohnaka K, Matsui D, Katsuura-Kamano S, Kubo M, Tanaka H, Kita Y.
  • Journal Title: J Nephrol Volume: 27(2) Issue: 2 Pages: 143-149
  • DOI: 10.1007/s40620-013-0025-0
  • Peer Reviewed
• [Journal Article] A polymorphism near MC4R gene (rs17782313) is associated with serum triglyceride levels in the general Japanese population: the J-MICC Study. (2014)
  • Author(s): Katsuura-Kamano S, Uemura H, Arisawa K, Yamaguchi M, Hamajima N, Wakai K, Okada R, Suzuki S, Taguchi N, Kita Y, Ohnaka K, Kairupan TS, Matsui D, Oze I, Mikami H, Kubo M, Tanaka H.
  • Journal Title: Endocrine. Volume: 47 Issue: 1 Pages: 81-89
  • DOI: 10.1007/s12020-014-0306-y
  • Peer Reviewed / Open Access
• [Journal Article] Associations between hOGG1 Ser326Cys polymorphism and increased body mass index and fasting glucose level in the Japanese general population. (2014)
  • Author(s): Hara M, Nakamura K, Nanri H, Nishida Y, Hishida A, Kawai S, Hamajima N, Kita Y, Suzuki S, Mantjoro EM, Ohnaka K, Uemura H, Matsui D, Oze I, Mikami H, Kubo M, Tanaka H; Japan Multi-Institutional Collaborative Cohort (J-MICC) Study Group.
  • Journal Title: J Epidemiol. Volume: 24 Pages: 379-384
  • NAID: 130004449419
  • Peer Reviewed / Open Access
• [Journal Article] Polymorphisms of genes involved in lipid metabolism and risk of chronic kidney disease in Japanese - cross-sectional data from the J-MICC study. (2014)
  • Author(s): Hishida A, Wakai K, Naito M, Suma S, Sasakabe T, Hamajima N, Hosono S, Horita M, Turin TC, Suzuki S, Kairupan TS, Mikami H, Ohnaka K, Watanabe I, Uemura H, Kubo M, Tanaka H; J-MICC Study Group.
  • Journal Title: Lipids Health Dis. Volume: 13 Issue: 1 Pages: 162-162
  • DOI: 10.1186/1476-511x-13-162
  • Peer Reviewed / Open Access
• [Presentation] 前向きコホート研究による胃がん高リスク群でのアミノ酸変化を伴う多型の解析 (2016)
  • Author(s): 滝口伸浩、中村洋子、三上春夫、永瀬 浩喜
  • Organizer: 第54回日本癌治療学会学術集会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2016-10-20
• [Presentation] Analysis of non-synonymous genetic variations associated with cancer risk by a prospective cohort study 前向きコホート研究によるがんの高リスク群でのアミノ酸変化を伴う多型の解析 (2016)
  • Author(s): Yohko Nakamura, Yoshiaki Maru, Yasutoshi Tatsumi, Kazuhiro Okumura, Sana Yokoi, Miki Ohira,, Yoshihiro Nabeya, Satoshi Fukasawa, Jin Katayama Hiroki Nagase, Haruo Mikami
  • Organizer: 第75回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2016-10-06
• [Presentation] 半導体次世代シークエンサーによるリスク集団特異的がん関連遺伝子多型解析 (2015)
  • Author(s): 中村洋子、丸喜明、巽康年、横井左奈、大平美紀,、中村友紀、高山喜美子、稲田潤子、片山稔、永瀬浩喜、三上春夫
  • Organizer: 第53回日本癌治療学会学術集会
  • Place of Presentation: 京都国際会館
  • Year and Date: 2015-10-29
• [Presentation] 半導体次世代シークエンサーによるリスク集団特異的がん関連遺伝子多型解析 (2015)
  • Author(s): 中村洋子、丸喜明、巽康年、横井左奈、大平美紀、中村友紀、高山喜美子、稲田潤子、田中尚武、山本尚人、鍋谷圭宏、滝口伸浩、植田健、片山稔、永瀬浩喜、三上春夫
  • Organizer: 日本人類遺伝学会第60回大会
  • Place of Presentation: 京王プラザホテル
  • Year and Date: 2015-10-14
• [Presentation] Analysis of cancer risk-associated genetic variations by using semiconductor-based next-gen sequencings in J-MICC study (2015)
  • Author(s): Yohko Nakamura, Yoshiaki Maru, Yasutoshi Tatsumi, Sana Yokoi, Miki Ohira, Yuki Nakamura, Kimiko Takayama, Junko Inada, Jin Katayama, Hiroki Nagase, Haruo Mikami
  • Organizer: 第74回日本癌学会学術集会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2015-10-08
• [Remarks] 千葉県がんセンター研究所
  • URL: http://www.pref.chiba.lg.jp/gan/kenkyujo/
• [Remarks] 千葉県がんセンター研究所
  • URL: http://www.pref.chiba.lg.jp/gan/kenkyujo/index.html