To prevent osteoporosis using food derived phytoestrogen

• Project/Area Number: 26460794
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Hygiene and public health
• Research Institution: Kanazawa University
• Principal Investigator: Kiyofumi Saijoh 金沢大学, 医学系, 教授 (00178469)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2014: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: ゲニステイン / メナキノン-4（MK-4） / BGLAP / CHAD / ALP3 / DPP4 / 骨芽細胞様 / MC3T3-E1細胞 / マウス骨芽細胞MC3T3E1 / vitamin K2 / estrogen / genistein / sonic hedgehog (SHH) / patched (PTC) / 骨粗鬆症 / QOL / sonic hedgehog(SHH) / patched(PTC)

Outline of Final Research Achievements

Bone is a basic component of the musculoskeletal system and is constantly remodeled due to the function of osteoblasts and osteoclasts. The bone remodeling decreased especially in women following menopause, accelerated bone loss and leas to osteoporosis. Osteoporosis is a serious social and health problem in a progressively aging society. Utilization of food-based nutrients like genistein, contained in soybeans, and menaqoinone-4, in dairy foods, are considered to be the most economical and easy way to prevent bone loss. In the present study, effect of genistein and menaquinone-4 on osteoblastic MC3T3-El cells were examined. We could isolate 21 mRNAs of which expression were increased more than three as much an control when genistein was supplied. Thereafter, co-effects of genistein and menaqoinone-4 were examined. We confirmed their simultaneous effectiveness on maintenance of using Chad and ALP.

Research Products

• [Journal Article] Genistein and menaquinone-4 treatment-induced alterations in the expression of mRNAs and their products are beneficial to osteoblastic MC3T3-El cell functions. (2017)
  • Author(s): MIDORI KATSUYAMA, MASASHI DEMURA, HIRONOBU KATSUYAMA, HIDEJI TANII, KIYOFUMI SAIJOH.
  • Journal Title: Molecular Medicine Reports (in press) Volume: 印刷中
  • Peer Reviewed / Open Access
• [Journal Article] Dynamic CCAAT/Enhancer Binding Protein–Associated Changes of DNA Methylation in the Angiotensinogen Gene (2014)
  • Author(s): Fen Wang, Masashi Demura, Yuan Cheng, Aoshuang Zhu, Shigehiro Karashima, Takashi Yoneda, Yoshiki Demura, Yuji Maeda, Mikio Namiki, Katsuhiko Ono, Yasuhiro Nakamura, Hironobu Sasano, Tadayuki Akagi, Masakazu Yamagishi, Kiyofumi Saijoh, Yoshiyu Takeda
  • Journal Title: Hypertension. Volume: 63(2) Issue: 2 Pages: 281-288
  • DOI: 10.1161/hypertensionaha.113.02303
  • Peer Reviewed / Open Access
• [Journal Article] Regulation of serine protease inhibitor Kazal type-5 (SPINK5) gene expression in the keratinocytes. (2014)
  • Author(s): Le NA1, Katsuyama M, Demura M, Tanii H, Katsuyama H, Saijoh K.
  • Journal Title: Environ Health Prev Med. Volume: 19(4) Issue: 4 Pages: 307-313
  • DOI: 10.1007/s12199-014-0393-7
  • Peer Reviewed / Open Access
• [Journal Article] Exacerbation of daily cough and allergic symptoms in adult patients with chronic cough by Asian Dust: A hospital-based study in Kanazawa (2014)
  • Author(s): Higashi T, Kambayashi Y , Ohkura N , Fujimura M, Nakanishi S, Yoshizaki T, Saijoh K , Hayakawa K, Kobayashi F , Michigami Y, Hitomi Y, Nakamura H
  • Journal Title: Atmospheric Environment Volume: 97 Pages: 537-543
  • Peer Reviewed / Open Access
• [Journal Article] Effects of Asian dust on daily cough occurrence in patients with chronic cough: a panel study (2014)
  • Author(s): Higashi T, Kambayashi Y , Ohkura N , Fujimura M, Nakai S, Honda Y, Saijoh K , Hayakawa K, Kobayashi F , Michigami Y, Olando AE , Hitomi Y, Nakamura H
  • Journal Title: Atmospheric Environment Volume: 92 Pages: 506-513
  • Peer Reviewed / Open Access
• [Journal Article] Asian dust (kosa) in allergic diseases. (2014)
  • Author(s): Higashi T, Kambayashi Y, Fujimura M, Ohkura N, Yoshizaki T, Nakanishi S, Saijoh K, Hayakawa K, Kobayashi F, Michigami Y, Hitomi Y, Nakamura H.
  • Journal Title: Earozoru Kenkyu (in Japanese) Volume: 29(S1) Pages: 212-217
  • NAID: 130003393430
  • Peer Reviewed / Open Access
• [Presentation] 某病院勤務者における高ストレス背景因子の検討 (2017)
  • Author(s): 勝山博信、佐藤友美、渡辺洋子、西條清史
  • Organizer: 第90回日本産業衛生学会
  • Place of Presentation: 東京都
  • Year and Date: 2017-05-11
• [Presentation] miRNA-mediated crosstalk between Wnt3a and TGFP3 in osteoblast differentiation (2017)
  • Author(s): Shigeko Fushimi, Tsutomu Nohno, Shin-ichiro Nishimatsu, Naoki Katase, Kumiko, Terada, Midori Katsuyama, Masashi Demura, Kiyofumi Saijoh, Hitoshi Nagatsuka, Hironobu Katsuyama.
  • Organizer: EXPERIMENTAL BIOLOGY 2017
  • Place of Presentation: Chicago McCormick Convention Center
  • Year and Date: 2017-04-22
  • Int'l Joint Research
• [Presentation] 水浸拘束ストレスマウスにおける筋委縮メカニズムの解明 (2016)
  • Author(s): 伏見滋子、濃野勉，寺田久美子、片瀬直樹、西松伸一郎、渡辺洋子、長塚仁、勝山碧、西條清史、勝山博信
  • Organizer: 第86回日本衛生学会 学術総会
  • Place of Presentation: 北海道
  • Year and Date: 2016-05-11
• [Presentation] Mouse Model of Muscle Atrophy with Water-Immersion Restraint Stress. (2016)
  • Author(s): Shigeko Fushimi, Tsutomu Nohno, Shin-ichiro Nishimatsu, Naoki Katase, Kumiko Terada, Yoko Watanabe, Yoko Ota, Midori Katsuyama, Kiyofumi Saijoh and Hironobu Katsuyama
  • Organizer: EXPERIMENTAL BIOLOGY 2016
  • Place of Presentation: San Diego Convention Center
  • Year and Date: 2016-04-02
  • Int'l Joint Research
• [Presentation] Mouse Model of Muscle Atrophy with Water-Immersion Restraint Stress. (2016)
  • Author(s): Shigeko Fushimi, Tsutomu Nohno, Shin-ichiro Nishimatsu, Naoki Katase, Kumiko Terada, Yoko Watanabe, Yoko Ota, Midori Katsuyama, Kiyofumi Saijoh and Hironobu Katsuyama
  • Organizer: EXPERIMENTAL BIOLOGY 2016
  • Place of Presentation: San Diego Convention Center（米国）
  • Year and Date: 2016-04-02
  • Int'l Joint Research
• [Presentation] SOINK5 Expression in Keratinocytes. (2015)
  • Author(s): M. Katsuyama, N.A. Le, M. Demura, H. Tanii, Y. Ota, H. Katsuyama, and K. Saijoh.
  • Organizer: EB 2015
  • Place of Presentation: Boston Convention and Exhibition Center
  • Year and Date: 2015-03-28 – 2015-04-01
• [Presentation] Effect of vitamin K2 on trabecular bone mass in senescence-accelerated mice P6 (SAMP6) with water-immersion restraint stress (2014)
  • Author(s): H. Katsuyama, S. Fushimi, K.Yamane, M. Tomita, Y.Watanabe, T. Okuyama, M, Katsuyama, S.R.Rahayu, and K. Saijoh.
  • Organizer: EB 2014
  • Place of Presentation: San Diego Convention Center
  • Year and Date: 2014-04-26 – 2014-04-30