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Methods for the determination of urinary biomarkers of exposure to pesticides

Research Project

Project/Area Number 26460799
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Hygiene and public health
Research InstitutionNagoya University

Principal Investigator

Ueyama Jun  名古屋大学, 医学系研究科(保健), 准教授 (00397465)

Co-Investigator(Renkei-kenkyūsha) KONDO Takaaki  名古屋大学, 大学院医学系研究科, 教授 (00195900)
KAMIJIMA Michihiro  名古屋市立大学, 大学院医学研究科, 教授 (80281070)
Research Collaborator SAITO Isao  当会コープ商品安全検査センター, 顧問
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords殺虫剤 / 網羅的解析 / ターゲット分析 / 高感度分析 / 職業的殺虫剤曝露 / ネオニコチノイド系殺虫剤
Outline of Final Research Achievements

We have developed the determination method for urinary metabolites of pesticides. Ninety and 74 urine samples have been collected from farmers and the control group, respectively. The numbers of urine samples obtained from pest control operators were 281. We are collecting a lot of urine samples from general population continuously. These samples have been analyzed by metabolome and chemical-target analysis. Five potential biomarkers have been detected at this time. The exposure biomarker for neonicotinoid and pyrethroid exposure were measured from the collected urine samples.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (31 results)

All 2017 2016 2015 2014 Other

All Int'l Joint Research (1 results) Journal Article (10 results) (of which Int'l Joint Research: 6 results,  Peer Reviewed: 10 results,  Open Access: 2 results,  Acknowledgement Compliant: 2 results) Presentation (20 results) (of which Int'l Joint Research: 4 results,  Invited: 1 results)

  • [Int'l Joint Research] 上海交通大学(中国)

    • Related Report
      2016 Annual Research Report
  • [Journal Article] Quantitative analysis of organophosphate insecticide metabolites in urine extracted from disposable diapers of toddlers in Japan2017

    • Author(s)
      Oya N, Ito Y, Hioki K, Asai Y, Aoi A, Sugiura Y, Ueyama J, Oguri T, Kato S, Ebara T, Kamijima M.
    • Journal Title

      Int J Hyg Environ Health.

      Volume: 220 Issue: 2 Pages: 209-216

    • DOI

      10.1016/j.ijheh.2016.10.009

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Modulation of immunological activity on macrophages induced by diazinon.2017

    • Author(s)
      Ogasawara N, Matsushima M, Kawamura N, Atsumi K, Yamaguchi T, Ochi H, Kusatsugu Y, Oyabu S, Hashimoto N, Hasegawa Y, Ueyama J, Kawabe T.
    • Journal Title

      Toxicology.

      Volume: 379 Pages: 22-30

    • DOI

      10.1016/j.tox.2017.01.014

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Effects of Paraoxonase 1 gene polymorphisms on organophosphate insecticide metabolism in Japanese pest control workers2016

    • Author(s)
      Hirotaka Sato, Yuki Ito, Jun Ueyama, Yuya Kano, Tomoya Arakawa, Masahiro Gotoh, Takaaki Kondo, Yuka Sugiura, Isao Saito, Eiji Shibata, Michihiro Kamijima
    • Journal Title

      Journal of Occupational Health

      Volume: 58 Issue: 1 Pages: 56-65

    • DOI

      10.1539/joh.15-0175-OA

    • NAID

      130005127681

    • ISSN
      1341-9145, 1348-9585
    • Related Report
      2016 Annual Research Report 2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Exposure characterization of three major insecticide lines in urine of young children in Japan―neonicotinoids, organophosphates, and pyrethroid2016

    • Author(s)
      Aya Osaka, Jun Ueyama, Takaaki Kondo, Hiroshi Nomura, Yuka Sugiura, Isao Saito, Kunihiko Nakane, Ayuko Takaishi, Hiroko Ogi, Shinya Wakusawa, Yuki Ito, Michihiro Kamijima
    • Journal Title

      Environmental Research

      Volume: 147 Pages: 89-96

    • DOI

      10.1016/j.envres.2016.01.028

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Temporal Levels of Urinary Neonicotinoid and Diaklyphosphate Concentrations in Japanese Women Between 1994-20112015

    • Author(s)
      Jun Ueyama, Kouji H. Harada, Akio Koizumi, Yuka Sugiura, Takaaki Kondo, Isao Saito, and Michihiro Kamijima
    • Journal Title

      Environmental Science & Technology

      Volume: 49 Issue: 24 Pages: 14522-14528

    • DOI

      10.1021/acs.est.5b03062

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Lack of Contribution of Multidrug Resistance-associated Protein and Organic Anion-transporting Polypeptide to Pharmacokinetics of Regorafenib, a Novel Multi-Kinase Inhibitor, in Rats.2015

    • Author(s)
      Hotta K, Ueyama J, Tatsumi Y, Tsukiyama I, Sugiura Y, Saito H, Matsuura K, Hasegawa T.
    • Journal Title

      Anticancer Research

      Volume: 35 Pages: 4681-4689

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Comparison of different urine pretreatments for biological monitoring of pyrethroid insecticides2015

    • Author(s)
      Toshima H, Yoshinaga J, Shiraishi H, Ito Y, Kamijima M, Ueyama J
    • Journal Title

      J Anal Toxicol

      Volume: 39 Pages: 133-133

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Biological Monitoring Method for Urinary Neonicotinoid Insecticides Using LC-MS/MS and Its Application to Japanese Adults2014

    • Author(s)
      Ueyama J, Nomura H, Kondo T, Saito I, Ito Y, Osaka A, Kamijima M.
    • Journal Title

      Journal of Occupational Health

      Volume: 56 Issue: 6 Pages: 461-468

    • DOI

      10.1539/joh.14-0077-OA

    • NAID

      130004704507

    • ISSN
      1341-9145, 1348-9585
    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] A revised method for determination of dialkylphosphate levels in human urine by solid-phase extraction and liquid chromatography with tandem mass spectrometry: application to human urine samples from Japanese children.2014

    • Author(s)
      Ueyama J, Saito I, Takaishi A, Nomura H, Inoue M, Osaka A, Sugiura Y, Hayashi Y, Wakusawa S, Ogi H, Inuzuka K, Kamijima M, Kondo T.
    • Journal Title

      Environ Health Prev Med

      Volume: 19 Issue: 6 Pages: 405-413

    • DOI

      10.1007/s12199-014-0407-5

    • NAID

      50009331968

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Contribution of plasma proteins, albumin and alpha 1-acid glycoprotein, to pharmacokinetics of a multi-targeted receptor tyrosine kinase inhibitor, sunitinib, in analbuminemic rats.2014

    • Author(s)
      Toyama Y, Ueyama J, Nomura H, Tsukiyama I, Saito H, Hisada T, Matsuura K, Hasegawa T
    • Journal Title

      Anticancer Res

      Volume: 34 Pages: 2283-2283

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Presentation] 使用済み紙おむつを用いた幼児におけるピレスロイド系殺虫剤の曝露評価2017

    • Author(s)
      上山 純、生田勇太郎、上田裕子、伊藤由起、榎原 毅、大矢奈穂子、加藤沙耶香、小栗朋子、庄司直人、上島通浩
    • Organizer
      第87回日本衛生学会学術総会
    • Place of Presentation
      宮崎
    • Year and Date
      2017-03-26
    • Related Report
      2016 Annual Research Report
  • [Presentation] 新規ピレスロイド系殺虫剤の尿中代謝物高感度測定法の開発2017

    • Author(s)
      上田裕子、上山 純、小田真也、生田勇太郎、青井亜里沙、近藤高明、上島通浩、涌沢伸哉、斎藤勲
    • Organizer
      第87回日本衛生学会学術総会
    • Place of Presentation
      宮崎
    • Year and Date
      2017-03-26
    • Related Report
      2016 Annual Research Report
  • [Presentation] 使用済み紙おむつを用いた幼児における有機リン系殺虫剤曝露量の測定2017

    • Author(s)
      大矢奈穂子、伊藤由起、榎原 毅、日置啓介、青井亜里沙、上山 純、加藤沙耶香、小栗朋子、庄司直人、上島通浩
    • Organizer
      第87回日本衛生学会学術総会
    • Place of Presentation
      宮崎
    • Year and Date
      2017-03-26
    • Related Report
      2016 Annual Research Report
  • [Presentation] 生物学的モニタリングによる殺虫剤曝露評価から健康リスク評価へ -地域・職域集団を対象とした基礎的検討-2017

    • Author(s)
      上山純
    • Organizer
      第87回日本衛生学会学術総会
    • Place of Presentation
      宮崎
    • Year and Date
      2017-03-26
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] Exposure characterization of three major insecticide lines in urine of young children in Japan - neonicotinoids, organophosphates, and pyrethroids2016

    • Author(s)
      Jun Ueyama, Aya Osaka, Takaaki Kondo, Hiroshi Nomura, Yuka Sugiura, Isao Saito, Kunihiko Nakane, Ayuko Takaishi, Hiroko Ogi, Shinya Wakusawa, Yuki Ito, Michihiro Kamijima
    • Organizer
      Conference of international society for environmental epidemiology and international society of exposure science-Asia Chapter 2016
    • Place of Presentation
      札幌
    • Year and Date
      2016-06-26
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ラットを用いた殺虫剤アセタミプリドおよび代謝物の 血中および尿中動態2016

    • Author(s)
      生田勇太郎, 上山純,青井亜里沙, 近藤高明, 涌澤伸哉, 伊藤由起, 上島通浩, 斎藤勲
    • Organizer
      第86回日本衛生学会学術総会
    • Place of Presentation
      旭川
    • Year and Date
      2016-05-11
    • Related Report
      2016 Annual Research Report
  • [Presentation] 使用済みオムツを用いた1歳半児における尿中有機リン系殺虫剤曝露量の測定2016

    • Author(s)
      大矢奈穂子、伊藤由起、日置啓介、浅井祐哉、青井愛里沙、上山純、小栗朋子、加藤沙耶香、榎原毅、上島通浩
    • Organizer
      第86回日本衛生学会学術総会
    • Place of Presentation
      旭川
    • Year and Date
      2016-05-11
    • Related Report
      2016 Annual Research Report
  • [Presentation] 尿中有機リン系殺虫剤代謝物測定の個人間変動と施設間差についての検討2016

    • Author(s)
      伊藤由起、上山純、中山祥嗣、磯部友彦、佐藤博貴、大矢奈穂子、仲井邦彦、上島通浩
    • Organizer
      第86回日本衛生学会学術総会
    • Place of Presentation
      旭川
    • Year and Date
      2016-05-11
    • Related Report
      2016 Annual Research Report
  • [Presentation] Measurement of concentrations of organophosphate insecticide metabolites in urine extracted from used diapers in 1.5-year-old children2016

    • Author(s)
      Naoko Oya, Yuki Ito, Keisuke Hioki, Yuya Asai, Arisa Aoi, Yuka Sugiura, Jun Ueyama, Tomoko Oguri, Sayaka Kato, Takeshi Ebara, Michihiro Kamijima
    • Organizer
      2nd international conference on human biomonitoring
    • Place of Presentation
      ベルリン
    • Year and Date
      2016-04-17
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Concentration s of urinary pyrethroid metabolites in Japanese women between 1994-20112016

    • Author(s)
      Jun Ueyama, Koouji H Harada, Akio Koizumi, Yutaro Ikuta, Takaaki Kondo, Isao Saito, Michihiro Kamijima
    • Organizer
      2nd international conference on human biomonitoring
    • Place of Presentation
      ベルリン
    • Year and Date
      2016-04-17
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Interlaboratory and intraindividual variability analysis in measurements of urinary metabolites of organophosphorus insecticides2016

    • Author(s)
      Yuki Ito, Jun Ueyama,Shoji Nakayama, Tomohiko Isobe, Hirotaka Sato, Naoko Oya, Koichi Yoshimasu, Kanami Tsuno, Nozomi Tatsuta, Kunihiko Nakai, Michihiro Kamijima
    • Organizer
      2nd international conference on human biomonitoring
    • Place of Presentation
      ベルリン
    • Year and Date
      2016-04-17
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 日本人妊婦の尿中ネオニコチノイド系農薬濃度2016

    • Author(s)
      飴田玲伽、青井亜里沙、上山純、西浜柚季子、 吉永淳、久田文、下平和久、加藤進昌
    • Organizer
      環境ホルモン学会 第19回研究発表会
    • Place of Presentation
      筑波
    • Related Report
      2016 Annual Research Report
  • [Presentation] バイオモニタリング手法を用いた職業的ネオニコチノイド系殺虫 剤の曝露評価2015

    • Author(s)
      上山純、末永隆次郎、永美大志、 夏川周介、柴田英治、近藤高明、 上島通浩、斎藤勲
    • Organizer
      平成27 年度日本産業衛生学会東海地方会学会
    • Place of Presentation
      名古屋大学医学部
    • Year and Date
      2015-11-14
    • Related Report
      2015 Research-status Report
  • [Presentation] 使用済みオムツを利用した尿中代謝物一斉分析法の開発2015

    • Author(s)
      杉浦友香、上山純、上田裕子、伊藤由起、涌澤伸哉、上島通浩
    • Organizer
      産業中毒・生物学的モニタリング研究会
    • Place of Presentation
      愛知県南知多
    • Year and Date
      2015-10-02
    • Related Report
      2015 Research-status Report
  • [Presentation] 日本人における尿中殺虫剤曝露指標濃度の過去20年間の経年推移2015

    • Author(s)
      上山純、原田浩二、杉浦友香、大坂彩、小泉昭夫、上島通浩
    • Organizer
      第85回日本衛生学会学術総会
    • Place of Presentation
      和歌山
    • Year and Date
      2015-03-26 – 2015-03-28
    • Related Report
      2014 Research-status Report
  • [Presentation] 使い捨てオムツを用いた尿中低分子代謝物類の網羅的分析2015

    • Author(s)
      杉浦友香、上山純、上田裕子、涌澤伸哉、上島通浩
    • Organizer
      第85回日本衛生学会学術総会
    • Place of Presentation
      和歌山
    • Year and Date
      2015-03-26 – 2015-03-28
    • Related Report
      2014 Research-status Report
  • [Presentation] 3歳児における尿中殺虫剤曝露指標の相関関係2015

    • Author(s)
      上山純、大坂彩、野村洸司、斎藤勲、近藤高明、 黄木弘子、高石亜有子、伊藤由起、上島通浩
    • Organizer
      第85回日本衛生学会学術総会
    • Place of Presentation
      和歌山
    • Year and Date
      2015-03-26 – 2015-03-28
    • Related Report
      2014 Research-status Report
  • [Presentation] 日本人における尿中ネオニコチノイド系殺虫剤とその代謝物の濃度分布2014

    • Author(s)
      上山純,野村洸司,斎藤勲,近藤高明,岩田豊人,村田勝敬,涌澤伸哉,上島通浩
    • Organizer
      第41回日本毒性学会学術年会
    • Place of Presentation
      神戸
    • Year and Date
      2014-07-02 – 2014-07-04
    • Related Report
      2014 Research-status Report
  • [Presentation] LC-MS/MSによる尿中のネオニコチノイド系殺虫剤同時定量分析法の開発2014

    • Author(s)
      上山純、野村洸司、斎藤勲、近藤高明、伊藤由起、上島通浩
    • Organizer
      第84回日本衛生学会学術総会
    • Place of Presentation
      岡山
    • Year and Date
      2014-05-25 – 2014-05-27
    • Related Report
      2014 Research-status Report
  • [Presentation] 日本人小児を対象とした尿中ピレスロイド系殺虫剤代謝物の高感度分析2014

    • Author(s)
      大坂彩、上山純、近藤高明、野村洸司、斎藤勲、犬塚君雄、黄木弘子、高石亜有子、上島通浩
    • Organizer
      第84回日本衛生学会学術総会
    • Place of Presentation
      岡山
    • Year and Date
      2014-05-25 – 2014-05-27
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2018-03-22  

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