| Project/Area Number |
26460806
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Hygiene and public health
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| Research Institution | The Ohara Memorial Institute for Science of Labour (2015-2016)
Osaka City University (2014) |
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Principal Investigator |
ENDO Ginji 公益財団法人大原記念労働科学研究所, 研究部, 客員研究員 (20160393)
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| Co-Investigator(Kenkyū-buntansha) |
山中 健三 日本大学, 薬学部, 教授 (50182572)
加藤 孝一 日本大学, 薬学部, 教授 (60246931)
市原 学 東京理科大学, 薬学部薬学科, 教授 (90252238)
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| Research Collaborator |
SHIMODA Yasuyo 日本大学, 薬学部, 助教 (70779420)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 胆管がん / 1,2-ジクロロプロパン / ジクロロメタン / ハロゲン化炭化水素 / 化学発がん / グルタチオンS-トランスフェラーゼ / 体細胞突然変異 / 衛生 / 遺伝毒性 |
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| Outline of Final Research Achievements |
The cytotoxicity of 1,2,3-trichloropropane (TCP) is higher than that of 1,2-dichloropropane (DCP). Induction of drug-metabolizing enzymes did not affect toxicity. The mRNA synthesis of GSH-regenerating enzymes is higher than those of CYPs and GSTs in TCP treated cultured cells, suggesting that the metabolic activation by GSH may be deeply involved in the toxicity rather than that by CYPs.
The experiment using human fetal liver-derived cholangiocytes showed that treatment with TNFα increased mRNA level of NFκB and an enzyme related with somatic cell hyper mutation and class switch recombination, suggesting the involvement of this enzyme with mutation of cholangiocytes.
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