Effect of neonicotinoid in the immature murine testes.

• Project/Area Number: 26460809
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Hygiene and public health
• Research Institution: Tokai University
• Principal Investigator: TERAYAMA Hayato 東海大学, 医学部, 准教授 (00384983)
• Co-Investigator(Kenkyū-buntansha): 坂部 貢 東海大学, 医学部, 教授 (70162302) ; 遠藤 整 東海大学, 医学部, 講師 (10550551) ; 内藤 宗和 愛知医科大学, 医学部, 教授 (10384984)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: ネオニコチノイド / 精巣 / マウス / 未成熟 / 性成熟 / 神経 / ネオ二コチノイド

Outline of Final Research Achievements

Neonicotinoids belong to a new and widely used pesticides, and are thought to be safe to humans. With mimic chemical structures to nicotine, neonicotinoids also have agonist activity at nicotine acetylcholine receptor (nAchR). However, it has been recently reported that neonicotinoids have been are implicated in a number of human health disorders. Actually, nAChR have is expressed in not only the nervous system but also the reproductive system. In the testis, spermatid / sperm appear by meiosis when reach puberty. Therefore, the environment inside the testis changes dramatically after the puberty. Until now, when administering neonicotinoid pesticides to adult mice, decrease of testosterone has been reported. However, there has been no report about the effect of neonicotinoid pesticide in juvenile stage. In present study, we immunotoxically analyzed the testicular environment of oral administration neonicotinoid pesticide in the immature mice.

Research Products

• [Journal Article] Induction of experimental autoimmune orchitis by immunization with xenogenic testicular germ cells in mice. (2017)
  • Author(s): Qu N, Terayama H, Hirayanagi Y, Kuramasu M, Ogawa Y, Hayashi S, Hirai S, Naito M, Itoh M.
  • Journal Title: J Reprod Immunol. Volume: 121 Pages: 11-16
  • DOI: 10.1016/j.jri.2017.04.006
  • Peer Reviewed / Open Access
• [Journal Article] Pathological effect of arterial ischaemia and venous congestion on rat testes (2017)
  • Author(s): Hirai S, Hatayama N, Naito M, Nagahori K, Kawata S, Hayashi S, Qu N, Terayama H, Shoji S, Itoh M
  • Journal Title: Sci Rep. Volume: 14;7(1) Issue: 1 Pages: 5422-5422
  • DOI: 10.1038/s41598-017-05880-2
  • Peer Reviewed / Open Access
• [Journal Article] A case of a cystic artery arising from the superior mesenteric artery with abnormal branching of the celiac trunk. (2017)
  • Author(s): Yakura T, Hayashi S, Terayama H, Miyaki T, Nakano T, Naito M.
  • Journal Title: BMC Res Notes Volume: 30;10(1) Issue: 1 Pages: 526-526
  • DOI: 10.1186/s13104-017-2858-4
  • Peer Reviewed / Open Access
• [Journal Article] Common and separate origins of the left and right inferior phrenic artery with a review of the literature. (2017)
  • Author(s): Terayama H, Yi SQ, Tanaka O, Kanazawa T, Suyama K, Kosemura N, Tetsu S, Yamazaki H, Sakamoto R, Kawakami S, Suzuki T, Sakabe K.
  • Journal Title: Folia Morphol Volume: 76(3) Issue: 3 Pages: 408-413
  • DOI: 10.5603/fm.a2017.0025
  • Peer Reviewed / Open Access
• [Journal Article] Chemical Sensitivity-The Frontier of Diagnosis and Treatment. (2016)
  • Author(s): Kou Sakabe, Minoru Kimura, Hayato Terayama, Masashi Tsunoda, Eriko Miyajima, Hirohisa Takano, Kenichi Azuma, Atsushi Mizukoshi, Tetsuya Matsuda, Chisato Mori, Mikio Miyata, Satoshi Ishikawa, Yoshiharu Aizawa
  • Journal Title: Japanese Journal of Clinical Ecology Volume: 25（2） Pages: 49-54
  • Peer Reviewed / Open Access
• [Journal Article] Specific autoantigens identified by sera obtained from mice that are immunized with testicular germ cells alone. (2016)
  • Author(s): Terayama H, Hirai S, Naito M, Qu N, Katagiri C, Nagahori K, Hayashi S, Sasaki H, Moriya S, Hiramoto M, Miyazawa K, Hatayama N, Li ZL, Sakabe K, Matsushita M, Itoh M.
  • Journal Title: Sci Rep. Volume: 18 Issue: 1 Pages: 35599-35599
  • DOI: 10.1038/srep35599
  • Peer Reviewed / Open Access
• [Journal Article] Acetamiprid Accumulates in Different Amounts in Murine Brain Regions. (2016)
  • Author(s): Terayama H, Endo H, Tsukamoto H, Matsumoto K, Umezu M, Kanazawa T, Ito M, Sato T, Naito M, Kawakami S, Fujino Y, Tatemichi M, Sakabe K.
  • Journal Title: Int J Environ Res Public Health Volume: 13(10) Issue: 10 Pages: 937-937
  • DOI: 10.3390/ijerph13100937
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Identification of a virulence determinant that is conserved in the Jawetz and Heyl biotypes in [Pasteurella] pneumotropica. (2016)
  • Author(s): Sasaki H, Ishikawa H, Terayama H, Asano R, Kawamoto E, Ishibashi H, Boot R
  • Journal Title: Pathogens and Disease Volume: 74 Issue: 6 Pages: ftw066-ftw066
  • DOI: 10.1093/femspd/ftw066
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] 免疫症候群－その他の免疫疾患を含めて－Ⅱ・Ⅴ．アレルギー性疾患 ・化学物質過敏症 (2016)
  • Author(s): 坂部 貢、寺山 隼人、金沢 輝久、木村 穣
  • Journal Title: 別冊日本臨牀 新領域別症候群シリーズ Volume: 35 Pages: 458-461
  • Peer Reviewed
• [Journal Article] 低用量フタル酸エステル曝露による自己免疫性精巣炎への影響 (2015)
  • Author(s): 寺山隼人、伊藤正裕、平井宗一、内藤宗和、曲寧、倉升三幸、小川夕輝、畑山直之、林省吾、平柳淑恵、隅山香織、金沢輝久、坂部貢
  • Journal Title: 臨床環境医学 Volume: 24(1) Pages: 48-57
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Anomalous connection of the left posterior renal vein with the left ascending lumbar vein in a Japanese cadaver. (2015)
  • Author(s): Terayama H, Yi SQ, Shoji S, Tanaka O, Kanazawa T, Kosemura N, Tamura M, Sekiguchi M, Naito M, Akamatsu T, Sakabe K.
  • Journal Title: Folia Morphol (Warsz) Volume: 74(4) Issue: 4 Pages: 544-547
  • DOI: 10.5603/fm.2015.0121
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Low-dose exposure to di-(2-ethylhexyl) phthalate (DEHP) increases susceptibility to testicular autoimmunity in mice. (2015)
  • Author(s): Hirai S, Naito M, Kuramasu M, Ogawa Y, Terayama H, Qu N, Hatayama N, Hayashi S, Itoh M.
  • Journal Title: Reproductive Biology Volume: 15(3) Issue: 3 Pages: 163-171
  • DOI: 10.1016/j.repbio.2015.06.004
  • Peer Reviewed
• [Journal Article] Busulfan pretreatment for transplantation of rat spermatogonia differentially affects immune and reproductive systems in male recipient mice. (2015)
  • Author(s): Hirayanagi Y, Qu N, Hirai S, Naito M, Terayama H, Hayashi S, Hatayama N, Kuramasu M, Ogawa Y, Itoh M.
  • Journal Title: Anat Sci Int. Volume: 90 Issue: 4 Pages: 264-274
  • DOI: 10.1007/s12565-014-0261-y
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Multi-Acupuncture Point Injections and Their Anatomical Study in Relation to Neck and Shoulder Pain Syndrome (So-Called Katakori) in Japan. (2015)
  • Author(s): Terayama H, Yamazaki H, Kanazawa T, Suyama K, Tanaka O, Sawada M, Ito M, Ito K, Akamatsu T, Masuda R, Suzuki T, Sakabe K.
  • Journal Title: PLoS One Volume: 10(6) Issue: 6 Pages: e0129006-e0129006
  • DOI: 10.1371/journal.pone.0129006
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] GRP78 suppresses lipid peroxidation and promotes cellular antioxidant levels in glial cells following hydrogen peroxide exposure. (2014)
  • Author(s): Suyama K, Watanabe M, Sakabe K, Otomo A, Okada Y, Terayama H, Imai T, Mochida J.
  • Journal Title: PLoS One Volume: 24;9(1) Issue: 1 Pages: e86951-e86951
  • DOI: 10.1371/journal.pone.0086951
  • Peer Reviewed / Open Access
• [Journal Article] Chronological changes of delayed-type hypersensitivity in mice immunised with testicular germ cells alone. (2014)
  • Author(s): Qu N, Naito M, Terayama H, Hirai S, Musha M, Itoh M.
  • Journal Title: Andrologia Volume: 46 Issue: 5 Pages: 556-563
  • DOI: 10.1111/and.12116
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Reproductive immunology and Immnotoxicology (2014)
  • Author(s): Terayama H, Sakabe K
  • Journal Title: ImmunoTox Letter Volume: 19(1) Pages: 3-4
  • Peer Reviewed
• [Journal Article] サイトカインによる精巣免疫特権の維持～IL-35の役割とその病態生理学的意義について～. (2014)
  • Author(s): 寺山隼人、伊藤正裕、内藤宗和、平井宗一、曲寧、畑山直之、林省吾、田村摩衣子、金沢輝久、善本隆之、坂部貢
  • Journal Title: 日本病態生理学会雑誌 Volume: 23(3) Pages: 46-49
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] 精巣内インターロイキン-35 の役割 (2014)
  • Author(s): 寺山隼人、善本隆之、内藤宗和、平井宗一、曲寧、畑山直之、林省吾、金沢輝久、隅山香織、坂部貢、伊藤正裕
  • Journal Title: 日本生殖免疫学会誌 Volume: 29(1) Pages: 13-23
  • NAID: 130005111145
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] Common and separate origins of the left and right inferior phrenic artery with a review of the literature. (2017)
  • Author(s): Hayato Terayama, Umezu Mai, Yuko Furuya, Kouichi Matsumoto, Kaori Suyama, Satoshi Kawakami, Noriyuki Kosemura, Kyoko Endo, Teruhisa Kanazawa, Osamu Tanaka, Kou Sakabe
  • Organizer: 第122回日本解剖学会総会・学術集会
  • Place of Presentation: 長崎大学医学部坂本キャンパス（長崎県長崎市）
  • Year and Date: 2017-03-28
• [Presentation] アセタミプリドの長期曝露による精巣の病態生理 (2017)
  • Author(s): 寺山隼人、曲寧、坂部貢
  • Organizer: 第27回日本病態生理学会大会
• [Presentation] アセタミプリドの長期曝露によるマウス精巣の男性ホルモンの変化 (2017)
  • Author(s): 寺山隼人、曲寧、遠藤整、立道昌幸、坂部貢
  • Organizer: 第24回日本免疫毒性学会学術年会
• [Presentation] 人工化学物質の生物への影響に関する地域啓発① 「農薬研究-ネオニコチノイド系農薬の裏表-」 (2017)
  • Author(s): 寺山隼人
  • Organizer: 東海大学To-Collaboプログラム大学推進プロジェクト「エコ・コンシャス計画 環境保全事業」シンポジウム
  • Invited
• [Presentation] 農薬は悪か否か-農薬の歴史を踏まえて- (2017)
  • Author(s): 寺山隼人
  • Organizer: ミューズ・フェスタ2018シンポジウム 「沈黙の春第二章？田んぼの生き物の危機」
  • Invited
• [Presentation] 農薬のサイエンス －ネオニコチノイド系農薬を中心に－ (2017)
  • Author(s): 寺山隼人
  • Organizer: 第3回生殖懇話会（第123回日本解剖学会総会・全国学術集会）
  • Invited
• [Presentation] ネオニコチノイド系農薬のサイエンス－研究の必要性ー (2017)
  • Author(s): 寺山隼人
  • Organizer: エココンシャス計画環境保全事業シンポジウム「環境保全型社会に向けた次世代育成の取り組み」
  • Place of Presentation: 東海大学湘南校舎13号館201教室（神奈川県平塚市）
  • Invited
• [Presentation] Acetamiprid as Neonicotinoid Pesticide Accumulates in Different Amounts in Murine Brain Regions (2016)
  • Author(s): Hayato Terayama, Hitoshi Endo, Kouichi Matsumoto, Mai Umezu, Teruhisa Kanazawa, Yasuhiro Fujino, Masayuki Tatemichi, Kou Sakabe
  • Organizer: 第19回環境ホルモン学会研究発表会
  • Place of Presentation: 文部科学省研究交流センター2F（茨城県つくば市）
  • Year and Date: 2016-12-08
• [Presentation] Testicular immune privilege and macrophage (2016)
  • Author(s): Ning Qu、Hayato Terayama、Shuichi Hirai、Shogo Hayashi、Naoyuki Hatayama、Miyuki Kuramasu、Yuki Ogawa、Kou Sakabe、Masahiro Itoh
  • Organizer: 第１２１回日本解剖学会総会・全国学術集会
  • Place of Presentation: 福島県・郡山市・ビッグパレットふくしま
  • Year and Date: 2016-03-27
  • Invited
• [Presentation] 精巣におけるマクロファージの免疫寛容機構 (2015)
  • Author(s): 平井宗一、寺山 隼人、曲寧、畑山直之、倉升三幸、小川夕輝、坂部 貢、伊藤正裕
  • Organizer: 第30回日本生殖免疫学会学術集会
  • Place of Presentation: 熊本県・熊本市・くまもと県民交流館パレア
  • Year and Date: 2015-11-21
  • Invited
• [Presentation] ネオ二コチノイド系農薬であるアセタミプリド曝露によるステロイド合成系への影響 (2015)
  • Author(s): 寺山隼人、遠藤整、金沢輝久、立道昌幸、坂部貢
  • Organizer: 第30回日本生殖免疫学会学術集会
  • Place of Presentation: 熊本県・熊本市・くまもと県民交流館パレア
  • Year and Date: 2015-11-21
• [Presentation] Importance of IL-35 to maintaining the immune privilege in the testis (2015)
  • Author(s): Hayato Terayama、Teruhisa Kanazawa、Kou Sakabe
  • Organizer: Tokai University Micro/Nano Enlightenment第5回学術講演会
  • Place of Presentation: 神奈川県・伊勢原市・東海大学伊勢原校舎 松前記念講堂、２号館地下１階レストラン望星台
  • Year and Date: 2015-08-25
• [Presentation] Reproductive immunotoxicology with special reference to male reproductive organ (2015)
  • Author(s): Hayato Terayama、Masahiro Itoh、Miyuki Kitaoka、Yuki Ogawa、Teruhisa Kanazawa、Kou Sakabe
  • Organizer: 第42回日本毒性学会学術年会・SOT-JSOT Exchange Promotion Program・-Progress in Immunotoxicology-
  • Place of Presentation: 石川県・金沢市・石川県立音楽堂、金沢市アートホール、ホテル日航金沢
  • Year and Date: 2015-06-29
  • Invited
• [Presentation] アセタミプリド曝露による精巣毒性 (2015)
  • Author(s): 寺山隼人、金沢輝久、田村摩衣子、古谷祐生子、坂部貢
  • Organizer: 第24回日本臨床環境医学会学術集会
  • Place of Presentation: 東京都・港区・北里大学薬学部コンベンションホール
  • Year and Date: 2015-06-06
• [Presentation] アセタミプリド曝露による中枢神経毒性 (2015)
  • Author(s): 寺山隼人、金沢輝久、田村摩衣子、古谷祐生子、坂部貢
  • Organizer: 第24回日本臨床環境医学会学術集会
  • Place of Presentation: 東京都・港区・北里大学薬学部コンベンションホール
  • Year and Date: 2015-06-06
• [Presentation] Acetamiprid affect the various gene expressions in murine testis and brain (2015)
  • Author(s): 寺山隼人, 金沢輝久, 田村摩衣子, 古谷祐生子, 遠藤整, 内藤宗和, 立道昌幸, 渡辺哲, 坂部貢
  • Organizer: 第85回日本衛生学会学術総会
  • Place of Presentation: 和歌山県民文化会館･ホテルアバローム紀の国
  • Year and Date: 2015-03-26 – 2015-03-28
• [Presentation] Variations in the vessels connecting the posterior tributary of the left renal vein to the left ascending lumbar vein without communicating with other renal veins in a Japanese cadaver (2015)
  • Author(s): 寺山隼人, 易勤, 小路直, 田中理, 金沢輝久, 小瀬村典之, 田村摩衣子, 関口雅樹, 内藤宗和, 坂部貢
  • Organizer: 第120回日本解剖学会総会・全国学術集会
  • Place of Presentation: 神戸国際会議場・展示場
  • Year and Date: 2015-03-21 – 2015-03-23
• [Presentation] ネオ二コチノイド系アセタミプリド曝露によるマウス精巣および脳への影響 (2014)
  • Author(s): 寺山隼人, 金沢輝久, 田村摩衣子, 遠藤整, 坂部貢
  • Organizer: 環境ホルモン学会第17回研究発表会
  • Place of Presentation: 東京大学山上会館
  • Year and Date: 2014-12-09 – 2014-12-10
• [Presentation] インターロイキン-35 による精巣免疫特権の維持 (2014)
  • Author(s): 寺山隼人, 善本隆之, 内藤宗和, 平井宗一, 曲寧, 畑山直之, 林省吾, 倉升三幸, 水戸部佳奈, 古澤純一, 溝口出, 毛塚剛司, 後藤浩, 金沢輝久, 坂部貢, 伊藤正裕
  • Organizer: 第92回東京医科大学・東京薬科大学免疫アレルギー研究会
  • Place of Presentation: 東京医科大学病院自主自学館
  • Year and Date: 2014-11-18
• [Presentation] 低用量フタル酸エステル曝露による自己免疫性精巣炎への影響 (2014)
  • Author(s): 寺山隼人, 坂部貢, 平井宗一, 内藤宗和, 曲寧, 倉升三幸, 小川夕輝, 畑山直之, 林省吾, 古谷祐生子, 金沢輝久, 伊藤正裕
  • Organizer: 第21回日本免疫毒性学会学術年会
  • Place of Presentation: 徳島文理大学国際会議場
  • Year and Date: 2014-09-11 – 2014-09-12
• [Presentation] Contribution of IL-35 to maintaining the immune privilege of testis (2014)
  • Author(s): Hayato Terayama, Masahiro Itoh, Shuichi Hirai, Munekazu Naito, Ning Qu, Maiko Tamura, Teruhisa Kanazawa, Kou Sakabe
  • Organizer: 第24回日本病態生理学会大会
  • Place of Presentation: 北九州国際会議場
  • Year and Date: 2014-08-08 – 2014-08-10
• [Presentation] 低用量フタル酸エステル曝露による自己免疫性精巣炎への影響 (2014)
  • Author(s): 寺山隼人、坂部 貢、平井宗一、内藤宗和、曲 寧、倉升三幸、小川夕輝、 畑山直之、林 省吾、隅山香織、金沢輝久、伊藤正裕
  • Organizer: 第23回日本臨床環境医学学会学術集会
  • Place of Presentation: 京都大学百周年時計台記念館国際交流ホール
  • Year and Date: 2014-06-14 – 2014-06-15
• [Presentation] 精巣のimmune privilegeにおけるインターロイキン-35の役割 (2014)
  • Author(s): 寺山 隼人, 内藤 宗和, 善本 隆之, 平井 宗一, 曲 寧, 倉升 三幸, 金沢 輝久, 隅山 香織, 坂部 貢, 伊藤 正裕
  • Organizer: 日本アンドロロジー学会第33回学術大会
  • Place of Presentation: 軽井沢プリンスホテルウエスト
  • Year and Date: 2014-06-12 – 2014-06-13
• [Presentation] Contribution of IL-35 to maintaining the testicular immune privilege (2014)
  • Author(s): Hayato Terayama, Shuichi Hirai, Takayuki Yoshimoto, Munekazu Naito, Miyuki Kuramasu, Ning Qu, Naoyuki Hatayama, Teruhisa Kanazawa, Kaori Suyama, Kou Sakabe, Masahiro Itoh
  • Organizer: 34th Annual Meeting of the American Society for Reproductive Immunology
  • Place of Presentation: Allegria Hotel (New York)
  • Year and Date: 2014-06-02 – 2014-06-05