METAGENOMICS OF MICROBIAL COMMUNITIES IN GALLBLADDER BILE FROM PATIENTS WITH GALLBLADDER CANCER OR CHOLELITHIASIS

Research Project

Project/Area Number	26460812
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	Hygiene and public health
Research Institution	Niigata University (2015-2016) Niigata University of Health and Welfare (2014)
Principal Investigator	TSUCHIYA Yasuo 新潟大学, 医歯学総合研究科, 客員研究員 (60334679)
Co-Investigator(Kenkyū-buntansha)	山本正治新潟医療福祉大学, 健康科学部, 教授 (40018693) 浅井孝夫新潟医療福祉大学, 医療技術学部, 助教 (60612736)
Research Collaborator	LOZA Ernest ボリビア・日本消化器センター VILLA-GOMEZ Guido ボリビア・日本消化器センター TRUJILLO Carlos ボリビア・日本消化器センター BAEZ Sergio ソテロデルリオ病院 OKUDA Kayo タカラバイオ株式会社 NOZAKI Ryoko 臨床検査技師
Project Period (FY)	2014-04-01 – 2017-03-31
Project Status	Completed (Fiscal Year 2016)
Budget Amount *help	¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000) Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000) Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000) Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords	胆嚢癌 / 胆石症 / 胆嚢胆汁 / 次世代シーケンサー / メタゲノム解析 / 16S rRNA / 胆嚢がん / メタゲノミクス / 胆汁試料 / 胆嚢がん患者 / 胆石症患者 / 16S rRNA領域 / 国際情報交換 / チリ / 胆汁
Outline of Final Research Achievements	Salmonella typhi and Helicobacter infections have been shown to increase risk of gallbladder cancer (GBC), but findings have been inconsistent. Other bacterial infections may also be associated with GBC. We aimed to investigate the microbial communities in gallbladder bile of patients with GBC and cholelithiasis (CL). DNA was extracted from bile in seven GBC patients and 30 CL patients, and the V3-V4 region of 16S rRNA was amplified. The sequencing results were compared with the 16S database. DNA was detected in the bile of three GBC (42.9%) and four CL patients (13.3%). As the predominant species, Fusobacterium nucleatum, Escherichia coli, and Enterobacter sp. were detected in bile from GBC patients. Those in bile from CL patients were Escherichia coli, Salmonella sp., and Enterococcus gallinarum. Whether the three bacteria detected in bile from GBC patients would associated with the development of GBC just like colorectal cancer remains unknown.