Molecular epidemiology and research on prevention and anti-viral agent against respiratory viruses for young children

• Project/Area Number: 26460819
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Hygiene and public health
• Research Institution: Osaka Institute of Public Health (2017); Osaka City Institute of Public Health and Environmental Sciences (2014-2016)
• Principal Investigator: Kaida Atsushi 地方独立行政法人 大阪健康安全基盤研究所, 微生物部, 主任研究員 (50372131)
• Co-Investigator(Kenkyū-buntansha): 山元 誠司 大阪市立環境科学研究センター, その他部局等, 研究員 (20649008) ; 奥 勇一郎 大阪市立環境科学研究センター, その他部局等, 研究員 (10456832) ; 久保 英幸 大阪市立環境科学研究センター, その他部局等, 研究副主幹 (20321937)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2014: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 分子疫学 / ウイルスゲノム / パラインフルエンザウイルス / ライノウイルス / エンテロウイルスD68 / 呼吸器感染症 / 乳幼児 / ウイルス / ゲノム解析 / 分子疫学解析 / エンテロウイルスD68型 / エンテロウイルス D68

Outline of Final Research Achievements

One of the major causative agents of respiratory tract infections among children is respiratory virus. Our previous study suggested that human rhinovirus (HRV) and human parainfluenza viruses (HPIVs) accounted for about 40% of the total detected respiratory viruses from specimens of patients with respiratory symptoms. This study focused on HRV and HPIV types 1, 2, and 3 and performed a molecular epidemiological analysis. Also, complete genome sequences of the enterovirus D68 strains detected during the study period were also analyzed and compared with strains identified from other areas in the world.

Research Products

• [Journal Article] Ten-Year Surveillance of Measles Virus from 2007–2016 in Osaka City, Japan (2018)
  • Author(s): Kaida A, Iritani N, Kanbayashi D, Yamamoto SP, Hirai Y, Hakui N, Fujimori R,
  • Journal Title: Japanese Journal of Infectious Diseases Volume: 71 Issue: 2 Pages: 152-154
  • DOI: 10.7883/yoken.JJID.2017.322
  • NAID: 130006517112
  • ISSN: 1344-6304, 1884-2836
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] 大阪での11年間にわたる麻疹ウイルス検出状況と遺伝子型推移（2007～2017年） (2018)
  • Author(s): 改田 厚
  • Journal Title: 臨床とウイルス Volume: 46 Pages: 11-17
• [Journal Article] Distinct genetic clades of enterovirus D68 detected in 2010, 2013, and 2015 in Osaka City, Japan (2017)
  • Author(s): Kaida A, Iritani N, Yamamoto SP, Kanbayashi D, Hirai Y, Togawa M, Amo K,
  • Journal Title: PLOS ONE Volume: 12 Issue: 9 Pages: e0184335-e0184335
  • DOI: 10.1371/journal.pone.0184335
  • Peer Reviewed / Open Access
• [Journal Article] エンテロウイルス D68 型の再出現と系統樹解析（2010～2015年）―大阪市― (2015)
  • Author(s): 改田 厚、入谷展弘、山元誠司、上林大起、平井有紀、西尾孝之、久保英幸、外川正生、天羽清子、圀府寺 美、西垣敏紀、塩見正司、浅井定三郎、影山 努、伯井紀隆、森 宏美、西 貴美、安井典子、桝田晴美、細井舞子、青木理恵、坂本徳裕、廣川秀徹、半羽宏之、松本健二、吉村高尚
  • Journal Title: 病原微生物検出情報 Volume: 36 Pages: 17-18
• [Journal Article] Associations between Co-Detected Respiratory Viruses in Children with Acute Respiratory Infections (2014)
  • Author(s): Atsushi Kaida, Hideyuki Kubo, Koh-ichi Takakura, Jun-ichiro Sekiguchi, Seiji P Yamamoto, Urara Kohdera, Masao Togawa, Kiyoko Amo, Masashi Shiomi, Minori Ohyama, Kaoru Goto, Atsushi Hase, Tsutomu Kageyama, Nobuhiro Iritani
  • Journal Title: Japanese Journal of Infectious Diseases Volume: 67 Issue: 6 Pages: 469-475
  • DOI: 10.7883/yoken.67.469
  • NAID: 130004712740
  • ISSN: 1344-6304, 1884-2836
  • Peer Reviewed
• [Journal Article] Frequent respiratory viral infections in a young child in a 27-month follow-up study (2014)
  • Author(s): Atsushi Kaida, Hideyuki Kubo, Nobuhiro Iritani, Seiji P Yamamoto, Atsushi Hase, Koh- Ichi Takakura, and Tsutomu Kageyema
  • Journal Title: JMM Case Reports Volume: - Issue: 4
  • DOI: 10.1099/jmmcr.0.003020
  • Peer Reviewed / Open Access
• [Journal Article] Real-time RT-PCR Assays for Discriminating Influenza B Virus Yamagata and Victoria Lineages (2014)
  • Author(s): Mina Nakauchi, Ikuyo Takayama, Hitoshi Takahashi, Kunihiro Oba, Hideyuki Kubo, Atsushi Kaida, Masato Tashiro, Tsutomu Kageyama
  • Journal Title: Journal of Virological Methods Volume: 205 Pages: 110-115
  • DOI: 10.1016/j.jviromet.2014.04.016
  • Peer Reviewed / Open Access
• [Presentation] 麻しん診断例における麻しんウイルス検出状況と遺伝子型推移（大阪市 2007～2016年） (2017)
  • Author(s): 改田 厚
  • Organizer: 第58回日本臨床ウイルス学会
• [Presentation] Single genetic clades of Enterovirus D68 strains in 2010, 2013, and 2015 in Osaka City, Japan (2016)
  • Author(s): Atsushi Kaida , Nobuhiro Iritani, Seiji P Yamamoto, Daiki Kanbayashi, Yuki Hirai, Urara Kohdera, Masao Togawa, Kiyoko Amo, Masashi Shiomi, Toshinori Nishigaki, Tsutomu Kageyama, Hideyuki Kubo
  • Organizer: 19th Annual Meeting of the European Society for Clinical Virology
  • Place of Presentation: リスボン（ポルトガル）
  • Year and Date: 2016-09-14
  • Int'l Joint Research
• [Presentation] 大阪市で検出されたエンテロウイルスD68型の分子疫学解析（2010～2015年） (2016)
  • Author(s): 改田 厚, 圀府寺 美, 天羽清子, 塩見正司
  • Organizer: 第57回日本臨床ウイルス学会
  • Place of Presentation: ホテル華の湯（福島県郡山市）
  • Year and Date: 2016-06-18
• [Presentation] 改田 厚、天羽清子 (2015)
  • Author(s): エンテロウイルス68型の再出現ー大阪市2013年ー
  • Organizer: 第56回日本臨床ウイルス学会
  • Place of Presentation: 岡山大学鹿田キャンパス
  • Year and Date: 2015-06-14
• [Presentation] Reemergence of enterovirus D68 in 2013, Osaka, Japan (2015)
  • Author(s): Atsushi Kaida, Seiji P Yamamoto, Nobuhiro Iritani, Urara Kohdera, Masao Togawa, Kiyoko Amo, Toshinori Nishigaki, Tsutomu Kageyama, Hideyuki Kubo
  • Organizer: 31st Clinical Virology Symposium
  • Place of Presentation: デイトナビーチ（アメリカ）
  • Year and Date: 2015-04-27
• [Presentation] 2013/14シーズンに分離されたインフルエンザウイルスAH3亜型株の性状解析－大阪市 (2014)
  • Author(s): 久保英幸、改田 厚、山元誠司、入谷展弘
  • Organizer: 第62回日本ウイルス学会学術集会
  • Place of Presentation: パシフィコ横浜（神奈川県 横浜市）
  • Year and Date: 2014-11-10 – 2014-11-12
• [Presentation] Frequent respiratory viral infections in a young child in a 27-month follow-up study (2014)
  • Author(s): Atsushi Kaida, Hideyuki Kubo, Nobuhiro Iritani, Seiji P. Yamamoto, Atsushi Hase, Tsutomu Kageyema.
  • Organizer: International Congress on Medical Virology
  • Place of Presentation: バンコク（タイ）
  • Year and Date: 2014-11-05 – 2014-11-07