Forensic pathogenesis based on gene expression expressed during death interval.

• Project/Area Number: 26460876
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Legal medicine
• Research Institution: Shiga University of Medical Science
• Principal Investigator: nishi katsuji 滋賀医科大学, 医学部, 客員教授 (60073681)
• Co-Investigator(Kenkyū-buntansha): 古川 智之 滋賀医科大学, 医学部, 准教授 (60422888) ; 森田 沙斗武 滋賀医科大学, 医学部, 非常勤講師 (80721894)
• Research Collaborator: SAKAGUCHI Ikuo ; OKUNAGA Hayato ; NAKAGAWA Tokiko
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 法医病理 / 脳・心関連 / 法医病理学

Outline of Final Research Achievements

We examined distribution of hypoxic related antigens, which are expressed during agonal duration in the brain and heart, for application to forensic pathogenesis and diagnosis. We could observed that (1) glycoconjugate deposits in the molecular layer of hippocampus from cognitive patients, (2) reperfusion in brain or heart causes intensive injury, (3) expression of HIF1α is increasing with length of agonal duration, (4) RBM3 which is protector item to hypothermia has an important role as vaso-expansion, and (5) RBM3 is independently expressed in the nuclei of Sabstantia nigra of individuals died from neck-compression deaths.

Research Products

• [Journal Article] Histochemical Characteristics of Myocardium Obtained from Two huge Cardiomegaly with over 1000g in Weight. (2016)
  • Author(s): S Furukawa, M Kataoka, S Morita, A Uno, M Hitosugi, H Matsumoto, K Nishi.
  • Journal Title: SM J Clin Med. Volume: 2(1) Pages: 1011-1015
  • Peer Reviewed / Open Access
• [Journal Article] Expression of RNA binding motif protein 3(RBM3) in the Substantia Nigra might be utilized to distinguish neck compression deaths from other deaths (2016)
  • Author(s): K Nishi, S Furukawa, S Morita, M Hitosugi, L Eberle
  • Journal Title: Rev of Alb Leg Med Volume: 12 Pages: 59-66
  • Peer Reviewed
• [Journal Article] Clinical Phenotype and Segregation of Mitochondrial 3243A> G mutation in 2 pairs of monozygotic twins, (2016)
  • Author(s): Maeda K, Kawai H, Sanada M, Terashima T, Ogawa N, Idehara R, Makiishi T, Yasuda H, Sato S, Hoshi K, Yahikozawa H, Nishi K, Itoh Y, Ogasawara K, Tomita K, Indo HP, Majima HJ
  • Journal Title: JAMA Neurolog Volume: 20 Issue: 8 Pages: 990-993
  • DOI: 10.1001/jamaneurol.2016.0886
  • Peer Reviewed
• [Journal Article] Postmortem Acute Death Obtained Brains without Reperfusion are Utilizable and Reliable to Examine the Expression of Hypoxia Related Antigens. (2016)
  • Author(s): K Nishi, S Furukawa, S Morita, M Hitosugi, L Eberle,
  • Journal Title: Alb J Med & Helth Sci Volume: 47 Pages: 18-27
  • Peer Reviewed / Open Access
• [Journal Article] Histochemical characteristics of myocardium obtained from two huge cardiomegaly with over 1000g in weight. (2016)
  • Author(s): Furukawa S, Kataoka M, Morita S, Uno A, Hitosugi M, Matsumoto H, Nishi K.
  • Journal Title: SMJ Clin Med. Volume: 2 Pages: 1011-1011
  • Peer Reviewed
• [Journal Article] Evaluation of the progressed stage of hypoxic and ischemic cascade pathway in the myocardium using antibodies against hypoxic related antigens. (2015)
  • Author(s): Furukawa S, Morita S, Sakaguchi I, Uno A, Nakagawa T, Hitosugi Ml Nishi K
  • Journal Title: J Adv, Life Natl Sci. Volume: 1 Pages: 30-34
  • Peer Reviewed
• [Journal Article] RBM3 and CIRP may play an important rple as a vasodilator. A preliminary study. (2015)
  • Author(s): Furukawa S, MoritaS, Wingenfeld L, Hitosugi M, Nishi K
  • Journal Title: Review of Albanian Legal Med. Volume: 11 Pages: 74-84
  • Peer Reviewed
• [Journal Article] Classification of contraction bands using immunohistochemistry (2015)
  • Author(s): Morita S, Furukawa S, Nishi K
  • Journal Title: Am J Forensic Med. Pathol. Volume: 36 Pages: 23-26
  • Peer Reviewed / Open Access
• [Journal Article] Histochemical difference of neurons in Substantia Nigra induced by global brain ischemia/hypoxia. (2015)
  • Author(s): Furukawa S, MoritaS, Hitosugi M, Wingenfeld L, Nishi K
  • Journal Title: AJMHS Volume: 46 Pages: 13-20
  • Peer Reviewed
• [Journal Article] Immunohistochemical evaluation of hypoxia markers in the myocardium (2014)
  • Author(s): Satomu Morita, Satoshi Furukawa, Kasuji Nishi
  • Journal Title: Aust J Forensic Sci Volume: 47 Issue: 2 Pages: 138-146
  • DOI: 10.1080/00450618.2014.906653
  • Peer Reviewed / Open Access
• [Journal Article] Immunohistochemical staining of the brain tissue obtained from a man with multiple focal brain infarctions and different staging. (2014)
  • Author(s): Satoshi Furukawa, Satomu Morita, Lisa Wingenfeld, Wakoto Matsuda, Tokiko Nakagawa, Ikuo Sakaguchi, Akari Takaya, Katsuji Nishi.
  • Journal Title: Anil Aggrawal's Internet Journal of Forensic Medicine and Toxicology. Volume: 15
  • Peer Reviewed / Open Access
• [Presentation] Mystical expression of HIF1α in the several regions of the brain and the heart muscle obtained from two self-strangulation cases might indicate the existence brain-heart axis. (2017)
  • Author(s): K Nishi
  • Organizer: 10th International Symposium of Advance in Legal Medicine,
  • Place of Presentation: Dusseldorf, Germany.
  • Year and Date: 2017-09-12
• [Presentation] Expression of RNA binding motif protein 3 (RBM3) in Substantia Nigra might be utilized to distinguish neck compression deaths from other deaths. (2016)
  • Author(s): S Furukawa, S Morita, H OkNishi K, Furukawa S, Morita S, Hitosugi M, Eberle Lunaga, L Wingenfeld, K Nishi,
  • Organizer: 95.Jahrestagung der Deutschen Gesellschaft fuer Rechtsmedizin.
  • Place of Presentation: Hidelberg. Germany.
  • Year and Date: 2016-08-30
• [Presentation] 重量1kg超え心肥大の2解剖例. (2016)
  • Author(s): 西 克治, 片岡真弓, 古川智之, 森田沙斗武, 宇野亜加里, 田村佳映, 辻野正樹, 荒木尚美, 一杉正仁, 松本博志.
  • Organizer: 第100次日本法医学会学術全国集会
  • Place of Presentation: 品川区
  • Year and Date: 2016-06-15
• [Presentation] Histochemical findings of neurons in substantia nigra induced by global brain ischemia/hypoxia I: Expression of hypoxia inducible factor-1α protein (2015)
  • Author(s): 西克治
  • Organizer: 第94回ドイツ法医学会
  • Place of Presentation: ライプチッヒ
  • Year and Date: 2015-09-16
• [Presentation] Histochemical changes in substantia nigra induced by global brain ischemia or hypoxia I: Expression of hypoxia inducing factor 1α (2015)
  • Author(s): Katsuji Nishi, Satoshi Furuksawa, Satomu Morita, Masahito Hitosugi, Lisa Wingenfeld.
  • Organizer: 94回ドイツ法医学会
  • Place of Presentation: ライプチッヒ
  • Year and Date: 2015-09-15 – 2015-09-19
• [Presentation] 黒質細胞は、頸部圧迫死や縊死の診断に応用出来るか (2015)
  • Author(s): 西克治
  • Organizer: 第99次日本法医学会学術全国集会
  • Place of Presentation: 高知
  • Year and Date: 2015-06-10
• [Presentation] 大動脈弁硬化性心肥大心筋の免疫組織 (2014)
  • Author(s): 西克治，古川智之，森田沙斗武，坂口生夫，中川季子，髙谷亜加里，一杉正仁
  • Organizer: 第31回日本法医学会学術中四国地方集会
  • Place of Presentation: 高松
  • Year and Date: 2014-11-01