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Development of monolithic silica for solid phase extraction and simultaneous extraction of drugs in forensic samples by using its monolithic silica

Research Project

Project/Area Number 26460880
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Legal medicine
Research InstitutionHiroshima University

Principal Investigator

Namera Akira  広島大学, 医歯薬保健学研究院(医), 准教授 (30284186)

Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords分析化学 / 薬物分析 / 前処理 / 法医学 / 中毒 / 法医中毒学 / 薬物 / 抽出
Outline of Final Research Achievements

The optimal monolithic silica in MonoSpin C18 for solid-phase extraction of drugs from whole blood samples was developed to facilitate high-throughput analysis. The silica monolith of 20 µm through-pore size, 10.4 nm mesopore size, and 17.3% carbon content achieved the best recovery of the target analytes in whole blood samples. The extraction proceeded with centrifugal force via centrifuging at 1000 rpm for 2 min, and the eluate was directly injected into the liquid chromatography - mass spectrometry system without any tedious steps such as evaporation of extraction solvents. Under optimized condition, low detection limits of 0.5 ng/mL and calibration ranges up to 2000 ng/m1 were obtained. The results indicate that the developed method based on the use of monolithic silica has the advantages of convenience and high efficiency, and can be successfully applied to detect drugs in whole blood samples.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (4 results)

All 2016 2015

All Presentation (4 results)

  • [Presentation] 全血薬物抽出用MonoSpin C18-CXの開発と事例への応用2016

    • Author(s)
      奈女良 昭
    • Organizer
      日本法中毒学会第35年会
    • Place of Presentation
      大阪産業創造館
    • Year and Date
      2016-07-01
    • Related Report
      2016 Annual Research Report
  • [Presentation] 全血薬物抽出用MonoSpin C18-CXの開発と事例への適用2016

    • Author(s)
      奈女良 昭
    • Organizer
      日本法中毒学会第35年会
    • Place of Presentation
      大阪府
    • Year and Date
      2016-07-01
    • Related Report
      2015 Research-status Report
  • [Presentation] 全血中薬物抽出用MonoSpinの開発と中毒事例への応用2015

    • Author(s)
      奈女良 昭
    • Organizer
      日本法中毒学会第34年会
    • Place of Presentation
      九州大学医学部百年講堂
    • Year and Date
      2015-06-26 – 2015-06-27
    • Related Report
      2014 Research-status Report
  • [Presentation] 血液からの危険ドラッグ抽出に適したモノスピンカラムの開発と中毒事例への応用2015

    • Author(s)
      奈女良 昭
    • Organizer
      第99次日本法医学会学術全国集会
    • Place of Presentation
      高知市文化プラザ
    • Year and Date
      2015-06-11
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2018-03-22  

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