| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
The optimal monolithic silica in MonoSpin C18 for solid-phase extraction of drugs from whole blood samples was developed to facilitate high-throughput analysis. The silica monolith of 20 µm through-pore size, 10.4 nm mesopore size, and 17.3% carbon content achieved the best recovery of the target analytes in whole blood samples. The extraction proceeded with centrifugal force via centrifuging at 1000 rpm for 2 min, and the eluate was directly injected into the liquid chromatography - mass spectrometry system without any tedious steps such as evaporation of extraction solvents. Under optimized condition, low detection limits of 0.5 ng/mL and calibration ranges up to 2000 ng/m1 were obtained. The results indicate that the developed method based on the use of monolithic silica has the advantages of convenience and high efficiency, and can be successfully applied to detect drugs in whole blood samples.
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