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Decitabine Inhibits Tumor Cell Proliferation in Epstein-Barr Virus-Associated Gastric Cancer

Research Project

Project/Area Number 26460938
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Gastroenterology
Research InstitutionYamaguchi University

Principal Investigator

NISHIKAWA Jun  山口大学, 医学(系)研究科(研究院), 教授 (00379950)

Co-Investigator(Kenkyū-buntansha) 末広 寛  山口大学, 医学(系)研究科(研究院), 准教授 (40290978)
小賀 厚徳  山口大学, 医学(系)研究科(研究院), 准教授 (90243633)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords胃癌 / EBウイルス / DNAメチル化 / 脱メチル化剤 / Epstein-Barr virus
Outline of Final Research Achievements

We investigated the effect of the DNA demethylating agent against EBV-associated gastric cancer (EBVaGC). Decitabine inhibited cell growth and induced G2/M arrest and apoptosis in EBVaGC cell line, SNU719. The cells treated with decitabine were significantly inhibited the expression of E-cadherin and cell motility. The promoter regions of p73 and RUNX3 were demethylated and their expression was up-regulated by decitabine. They enhanced the expression of p21. P21 induced apoptosis and epithelial-methenchymal transition (EMT) inhibition through downregulation of c-Myc. The expression of BZLF1 was induced by decitabine in SNU719. Induction of EBV lytic infection was alternative way to cause apoptosis of the host cells. This study is the first report to reveal the effectiveness of a demethylating agent in inhibiting tumor cell proliferation and suppressing the expression of E-cadherin in EBV-associated gastric cancer.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (13 results)

All 2017 2016 2015 2014

All Journal Article (3 results) (of which Peer Reviewed: 2 results,  Open Access: 2 results) Presentation (9 results) Book (1 results)

  • [Journal Article] Decitabine inhibits tumor cell proliferation and up-regulates E-cadherin expression in Epstein-Barr virus-associated gastric cancer.2017

    • Author(s)
      Nakamura M, Nishikawa J, Saito M, Sakai K, Sasaki S, Hashimoto S, Okamoto T, Suehiro Y, Yamasaki T, Sakaida I.
    • Journal Title

      J Exp Clin Cancer Res.

      Volume: 89(3) Issue: 3 Pages: 508-17

    • DOI

      10.1002/jmv.24634

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Epstein-barr virus in gastric carcinoma.2014

    • Author(s)
      Nishikawa J, Yoshiyama H, Iizasa H, Kanehiro Y, Nakamura M, Nishimura J, Saito M, Okamoto T, Sakai K, Suehiro Y, Yamasaki T, Oga A, Yanai H, Sakaida I.
    • Journal Title

      Cancers

      Volume: 6 Issue: 4 Pages: 2259-2274

    • DOI

      10.3390/cancers6042259

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] EBウイルス関連胃癌2014

    • Author(s)
      西川 潤、中村宗剛、永尾未怜、西村純一、橋本真一、岡本健志、坂井田功
    • Journal Title

      G. I. Research

      Volume: 22 Pages: 460-464

    • Related Report
      2014 Research-status Report
  • [Presentation] Decitabine Inhibits Tumor Cell Proliferation and Up-regulates E-cadherin Expression in Epstein-Barr Virus-Associated Gastric Cancer2017

    • Author(s)
      中村宗剛、西川 潤
    • Organizer
      第89回日本胃癌学会総会
    • Place of Presentation
      広島国際会議場(広島県・広島市)
    • Year and Date
      2017-03-08
    • Related Report
      2016 Annual Research Report
  • [Presentation] EBウイルス関連胃癌の発生におけるヘリコバクターピロリ胃炎の意義2016

    • Author(s)
      柳井秀雄、西川 潤、村上知之
    • Organizer
      第102回日本消化器病学会総会
    • Place of Presentation
      京王プラザホテル(東京都・新宿区)
    • Year and Date
      2016-04-21
    • Related Report
      2016 Annual Research Report
  • [Presentation] EBウイルス関連胃癌の発生機序と特異的治療について2016

    • Author(s)
      西川 潤、柳井秀雄、坂井田功
    • Organizer
      第102回日本消化器病学会総会
    • Place of Presentation
      京王プラザホテル(東京都・新宿区)
    • Year and Date
      2016-04-21
    • Related Report
      2016 Annual Research Report
  • [Presentation] Epstain-Barrウイルス関連胃癌に対する脱メチル化剤の有用性2015

    • Author(s)
      中村 宗剛、西川 潤ら
    • Organizer
      第57回日本消化器病学会大会
    • Place of Presentation
      グランドプリンスホテル新高輪 東京都港区
    • Year and Date
      2015-10-08
    • Related Report
      2015 Research-status Report
  • [Presentation] Epstain-Barrウイルス関連胃癌に対する脱メチル化剤の有用性2015

    • Author(s)
      中村宗剛、西川 潤ら
    • Organizer
      第12回EBV研究会
    • Place of Presentation
      ビッグハート出雲 島根県出雲市
    • Year and Date
      2015-07-20
    • Related Report
      2015 Research-status Report
  • [Presentation] Epstein-Barr virus陽性胃癌の臨床像と治療への展開2015

    • Author(s)
      佐々木翔、西川 潤ら
    • Organizer
      第101回日本消化器病学会総会
    • Place of Presentation
      仙台国際センター 宮城県仙台市
    • Year and Date
      2015-04-23
    • Related Report
      2015 Research-status Report
  • [Presentation] EBV関連胃癌の分子生物学的特徴と治療戦略-特異的なメチル化遺伝子の同定と脱メチル化剤への影響を通して-2014

    • Author(s)
      中村宗剛、西川 潤、西村純一、永尾未怜、佐々木翔、橋本真一、岡本健志、酒井幸平、末広 寛、山﨑隆弘、坂井田功
    • Organizer
      第18回中国消化管スタディーグループ研究会
    • Place of Presentation
      米子(米子全日空ホテル)
    • Year and Date
      2014-11-28
    • Related Report
      2014 Research-status Report
  • [Presentation] EBV関連胃癌の分子生物学的特徴と治療戦略2014

    • Author(s)
      中村宗剛、西川 潤、佐々木翔、永尾未怜、西村純一、橋本真一、岡本健志、酒井幸平、末広 寛、山﨑隆弘、坂井田功
    • Organizer
      山口消化管フォーラム2014
    • Place of Presentation
      山口(山口グランドホテル)
    • Year and Date
      2014-10-28
    • Related Report
      2014 Research-status Report
  • [Presentation] EBV陽性胃癌に対する脱メチル化剤の抗腫瘍効果と上皮間葉移行への影響2014

    • Author(s)
      中村宗剛、西川 潤、西村純一、岡本健志、酒井幸平、末広 寛、坂井田功
    • Organizer
      第100回日本消化器病学会総会
    • Place of Presentation
      東京(東京国際フォーラム)
    • Year and Date
      2014-04-23 – 2014-04-25
    • Related Report
      2014 Research-status Report
  • [Book] EBウイルス関連胃癌2016

    • Author(s)
      柳井秀雄、西川潤、吉山裕規、清水則夫
    • Total Pages
      77
    • Publisher
      診断と治療社
    • Related Report
      2016 Annual Research Report

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Published: 2014-04-04   Modified: 2018-03-22  

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