| Project/Area Number |
26460942
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | Oita University |
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Principal Investigator |
Kodama Masaaki 大分大学, 福祉健康科学部, 教授 (20332893)
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| Co-Investigator(Kenkyū-buntansha) |
村上 和成 大分大学, 医学部, 教授 (00239485)
沖本 忠義 大分大学, 医学部, 講師 (90381037)
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| Project Period (FY) |
2014-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | Helicobacter pylori / 除菌後胃癌 / 癌遺伝子 / 次世代シーケンサー解析 / TP53 / PDGFRA / H. pylori / gastric cancer / PDGRA / H. pylori除菌後胃癌 / 次世代シーケンサー / 萎縮性胃炎 |
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| Outline of Final Research Achievements |
Exhaustive genetic characteristics of gastric cancer (GC) after H. pylori eradication was elucidated with using next-generation sequencing. 20 subjects (5: well differentiated GC before eradication, 5: well differentiated GC after eradication, 6: undifferentiated GC before eradication, 4: undifferentiated after eradication) were evaluated with using the Ion Ampliseq V2 Cancer Hot spot panel (Life Technologies). Genetic mutation was found in more than 80% subjects in oncogene APC, CSF1R, FGFR3, FLT3, KDR, PDGFRA, RET, STK11, TP53. Number of hotspot mutation of well differentiated GC group showed significantly few than the other group. In addition, only well-differentiated GC after eradication group did not detect the hotspot mutation of PDGFRA at all. GC after H. pylori eradication had different characteristics. Especially, the present result showed that well differentiated GC after eradication was considered to have the different carcinogenic mechanism genetically.
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