Identification of gastric cancer risk factors after H. pylori eradication by evaluating exhaustive genetic analysis with using next-generation sequencing.
| Project/Area Number |
26460942
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | Oita University |
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Principal Investigator |
Kodama Masaaki 大分大学, 福祉健康科学部, 教授 (20332893)
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| Co-Investigator(Kenkyū-buntansha) |
村上 和成 大分大学, 医学部, 教授 (00239485)
沖本 忠義 大分大学, 医学部, 講師 (90381037)
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| Project Period (FY) |
2014-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | Helicobacter pylori / 除菌後胃癌 / 癌遺伝子 / 次世代シーケンサー解析 / TP53 / PDGFRA / H. pylori / gastric cancer / PDGRA / H. pylori除菌後胃癌 / 次世代シーケンサー / 萎縮性胃炎 |
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| Outline of Final Research Achievements |
Exhaustive genetic characteristics of gastric cancer (GC) after H. pylori eradication was elucidated with using next-generation sequencing. 20 subjects (5: well differentiated GC before eradication, 5: well differentiated GC after eradication, 6: undifferentiated GC before eradication, 4: undifferentiated after eradication) were evaluated with using the Ion Ampliseq V2 Cancer Hot spot panel (Life Technologies). Genetic mutation was found in more than 80% subjects in oncogene APC, CSF1R, FGFR3, FLT3, KDR, PDGFRA, RET, STK11, TP53. Number of hotspot mutation of well differentiated GC group showed significantly few than the other group. In addition, only well-differentiated GC after eradication group did not detect the hotspot mutation of PDGFRA at all. GC after H. pylori eradication had different characteristics. Especially, the present result showed that well differentiated GC after eradication was considered to have the different carcinogenic mechanism genetically.
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Report
(5 results)
Research Products
(111 results)
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[Presentation] 17 cases of helicobacter pylori infection negative gastric cancer2017
Author(s)
Okamoto K, Kodama M, Okimoto T, Ogawa R, Fukuda K, Matsunari O, Mistutaka S, Mizukami K, Murakami K
Organizer
World Congress of Gastrointestinal Endoscopy ENDO 2017
Place of Presentation
Hyderabad International Convention Center, Hyderabad, India
Year and Date
2017-02-16
Related Report
Int'l Joint Research
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[Presentation] The relations of endoscopic findings and eradication effectiveness for H.pylori infected Immune Thrombocytopenic Purpura (or Immune thrombocytopenia).2017
Author(s)
Tadayoshi Okimoto, Kazuhiro Mizukami, Ryo Ogawa, Kazuhisa Okamoto, Mitsutaka Shuto, Kensuke Fukuda, Masaaki Kodama, Kazunari Murakami
Organizer
World Congress of Gastroenterology (WCOG) ACG (2017), 10/13-18, 2017, Orland, FL, USA.
Related Report
Int'l Joint Research
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[Presentation] Utility of gastroendoscopy for subjects with serum H.pylori antibody (IgG) titer equal or more than 3.0 U/ml- Study of endoscopic findings for titers between 3.0 and 9.9 U/ml (so-called “gray zone”)2016
Author(s)
Shuto M, Mizukami K, Murakami K, Matsunari O, Okamoto K, Okimoto T, Kodama M, Yamaoka Y, Takigami S, Nonaka Y, Fujioka T
Organizer
XXIXth International Workshop on Helicobacter and Microbiota in Inflammation and Cancer
Place of Presentation
Magdeburug, Germany
Year and Date
2016-09-15
Related Report
Int'l Joint Research
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[Presentation] Relationship between endoscopic atrophic classification and histological atrophy and intestinal metaplasia before and after H. pylori eradication.2016
Author(s)
Masaaki Kodama, Tadayoshi Okimoto, Hisanori Abe, Ryo Ogawa, Kazuhisa Okamoto, Kazuhiro Mizukami, Wataru Soma, Takashi Abe, Takayuki Nagai, Tsuyoshi Arita, Kazunari Murakami.
Organizer
APDW 2016; Asian Pacific Digestive Week
Place of Presentation
Kobe Convention Center, Kobe, Japan.
Related Report
Int'l Joint Research
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