Analysis of Lgr5-positive cells in the intestinal metaplasia

Research Project

Project/Area Number	26460948
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	Gastroenterology
Research Institution	Jichi Medical University
Principal Investigator	Hirosawa Takuya 自治医科大学, 医学部, 助教 (50570660)
Co-Investigator(Kenkyū-buntansha)	武藤弘行自治医科大学, 医学部, 教授 (50322392) 坂本博次自治医科大学, 医学部, 講師 (50536175)
Project Period (FY)	2014-04-01 – 2017-03-31
Project Status	Completed (Fiscal Year 2016)
Budget Amount *help	¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000) Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000) Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000) Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords	Lgr5
Outline of Final Research Achievements	Intestinal metaplasia is precancerous lesion of the stomach. We investigated the expression of Lgr5 in intestinal metaplasia and gastric carcinoma. By crossbreeding Cdx2-transgenic mouse and Lgr5-EGFP-ires-CreERT2 mouse, Lgr5-expression in intestinal metaplasia and gastric carcinoma was identified. By crossbreeding Lgr5-EGFP-ires-CreERT2-mouse, ROSA26-Loxp-stop-Loxp-LacZ-mouse, Cdx2-transgenic mouse, it was suggested that Lgr5 is induced by Cdx2.