| Project/Area Number |
26460959
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
角田 洋一 東北大学, 大学病院, 助教 (50509205)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | エピゲノム / アリル特異的DNAメチル化 / 炎症性腸疾患 / 疾患感受性遺伝子 |
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| Outline of Final Research Achievements |
We expect that SNPs in the susceptibility loci for inflammatory bowel disease (IBD) affect the DNA methylation around the SNPs in a cis manner and alter the gene expression. We also expect that this alteration is one of the mechanisms how associated SNPs affect the susceptibility to multifactorial diseases. In this study, allele specific DNA methylations of the genome DNA of lamina propria T cell were examined using Japonica array. We determined 218 sites of allele specific DNA methylation in the genome except imprinting regions. Among 218 regions, two are in the IBD susceptibility loci. In one of the two region, differences of the m-RNA expression between alleles were determined. In this study, we showed, for the first time, that the allele specific DNA methylation and allele specific expression exist in the susceptibility gene for IBD.
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