Elucidation of the role of lysophosphatidic acid receptors in Th2 type inflammatory bowel disease
| Project/Area Number |
26460960
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | Akita University |
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Principal Investigator |
AKAHOSHI NORIYUKI 秋田大学, 医学(系)研究科(研究院), 助教 (70534551)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | リゾホスファチジン酸 / 炎症性腸炎 / LPA5 / マスト細胞 / GPCR |
|---|
| Outline of Final Research Achievements |
One of the receptors for lysophosphatidic acid (LPA) having various bioactivities, LPA5 is widely expressed in the peripheral immune cells, including intestinal immune cells and mast cells. We induced various colitis models to LPA5 deficient mice, and as a result, the Th2 response predominant oxazolone colitis model worsened in LPA5 deficient mice. In association with Th2 response, exacerbations were observed in systemic passive anaphylaxis responses involving mast cells in LPA5 deficient mice. FACS analysis of peritoneal lavage fluid revealed that the number of connective tissue type mast cells was comparable between LPA5 deficient mice and wild type, but the mast cells of LPA5 deficient mice had high SSCs indicating cell density, and FcεRIα was highly expressed. In the peritoneal lavage fluid, the number of macrophages decreased and B-2 cells increased. These results suggested that LPA5 deficient mice may be biased towards Th2 dominance.
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Report
(4 results)
Research Products
(8 results)
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[Journal Article] Fibroblastic reticular cell-derived lysophosphatidic acid regulates confined intranodal T-cell motility.2016
Author(s)
Takeda A, Kobayashi D, Aoi K, Sasaki N, Sugiura Y, Igarashi H, Tohya K, Inoue A, Hata E, Akahoshi N, Hayasaka H, Kikuta J, Scandella E, Ludewig B, Ishii S, Aoki J, Suematsu M, Ishii M, Takeda K, Jalkanen S, Miyasaka M, Umemoto E.
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Journal Title
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Hyperhomocysteinemia abrogates fasting-induced cardioprotection against ischemia/reperfusion by limiting bioavailability of hydrogen sulfide anions.2015
Author(s)
Nakano S, Ishii I, Shinmura K, Tamaki K, Hishiki T, Akahoshi N, Ida T, Nakanishi T, Kamata S, Kumagai Y, Akaike T, Fukuda K, Sano M, Suematsu M.
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Journal Title
J Mol Med (Berl).
Volume: 印刷中
Issue: 8
Pages: 879-889
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Neutral aminoaciduria in cystathionine β-synthase-deficient mice; an animal model of homocystinuria.2014
Author(s)
Akahoshi N, Kamata S, Kubota M, Hishiki T, Nagahata Y, Matsuura T, Yamazaki C, Yoshida Y, Yamada H, Ishizaki Y, Suematsu M, Kasahara T, Ishii I.
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Journal Title
Am J Physiol Renal Physiol.
Volume: 306(12)
Issue: 12
Pages: F1462-F1476
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Presentation] シスタチオニンβシンターゼ欠損マウス(ホモシスチン尿症モデル)における中性アミノ酸尿2014
Author(s)
赤星 軌征, 鎌田 祥太郎, 久保田 将史, 菱木 貴子, 長畑 善子, 松浦 友美, 山崎 千穂, 吉田 由佳, 山田 秀典, 石崎 泰樹, 末松 誠, 笠原 忠, 石井 功
Organizer
第87回日本生化学会大会
Place of Presentation
京都
Year and Date
2014-10-15 – 2014-10-18
Related Report
