| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
One of the receptors for lysophosphatidic acid (LPA) having various bioactivities, LPA5 is widely expressed in the peripheral immune cells, including intestinal immune cells and mast cells. We induced various colitis models to LPA5 deficient mice, and as a result, the Th2 response predominant oxazolone colitis model worsened in LPA5 deficient mice. In association with Th2 response, exacerbations were observed in systemic passive anaphylaxis responses involving mast cells in LPA5 deficient mice. FACS analysis of peritoneal lavage fluid revealed that the number of connective tissue type mast cells was comparable between LPA5 deficient mice and wild type, but the mast cells of LPA5 deficient mice had high SSCs indicating cell density, and FcεRIα was highly expressed. In the peritoneal lavage fluid, the number of macrophages decreased and B-2 cells increased. These results suggested that LPA5 deficient mice may be biased towards Th2 dominance.
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