molecular biological analysis of Sessile serrated adenoma/polyp in the proximal colon

• Project/Area Number: 26460974
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Gastroenterology
• Research Institution: Kumamoto University
• Principal Investigator: Shono Takashi 熊本大学, 医学部附属病院, 助教 (40632667)
• Co-Investigator(Kenkyū-buntansha): 渡邊 丈久 熊本大学, 大学院生命科学研究部(医), 助教 (20634843) ; 直江 秀昭 熊本大学, 医学部附属病院, 助教 (30599246) ; 佐々木 裕 熊本大学, 大学院生命科学研究部(医), 教授 (70235282)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2014: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
• Keywords: 大腸癌 / 発癌 / 大腸ポリープ / 内視鏡 / 消化器 / miRNA / プロテオミクス / SSAP / 内視鏡治療 / ﾌﾟﾛﾃｵﾐｸｽ

Outline of Final Research Achievements

We resected 3 SSAPs with cancer in the proximal colon with use of endoscopic submucosal dissection (ESD). These specimens were divided into normal mucosa, SSAP and cancer, using with Laser Micro-Dissection method (LMD). DNA and RNA were extracted from each section, respectively. Especially, microRNA (miRNA) quantification was performed.
We molecularly analyzed extracted miRNAs and detected variable miRNAs in a characteristic manner during the process of carcinogenesis.

Research Products

• [Journal Article] Incidence and risk factors of synchronous colorectal cancer in patients with esophageal cancer: an analysis of 480 consecutive colonoscopies before surgery (2016)
  • Author(s): Yoshida N, Tamaoki Y, Baba Y, Sakamoto Y, Miyamoto Y, Iwatsuki M, Shono T, Miyamoto H, Imuta M, Kurashige J, Sawayama H, Tokunaga R, Watanabe M, Sasaki Y, Yamashita Y, Baba H.
  • Journal Title: Int J Clin Oncol Volume: 6 Issue: 6 Pages: 1079-1084
  • DOI: 10.1007/s10147-016-1015-8
  • Peer Reviewed
• [Presentation] 当院において大腸ESDを施行した表在型大腸腫瘍156例の検討 (2014)
  • Author(s): 庄野 孝
  • Organizer: 第39回日本大腸肛門症学会九州地方会
  • Place of Presentation: 佐賀市
  • Year and Date: 2014-09-23