| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
Here we investigated the role of numerous fibroblasts in the inflamed region on fibrosis, focusing on the activation of the expected NF-κB pathway, which is particularly relevant. NF-κB deficient mice specific for myofibroblasts considered to be involved in fibrosis were prepared, and dextran sodium sulfate (DSS) intestinal inflammation model and intestinal allograft model were examined. In vitro studies, we used fetal embryonic fibroblast cells. As a result, the number of myofibroblast cells induced by inflammation was less in knockout mice in intestinal inflammation model. αSMA and TGFβ were attenuated in knockout mice in transplanted intestinal tract. In the examination with MEF, expression of TGFβ and inflammatory cytokine by inflammatory stimulation was decreased in knockout MEF, suggesting that the NF-kB pathway was involved in fibrosis formation.
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