| Project/Area Number |
26460987
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Gastroenterology
|
|---|
| Research Institution | Tokyo Medical and Dental University |
|---|
Principal Investigator |
Itsui Yasuhiro 東京医科歯科大学, 医学部附属病院, 講師 (20401341)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
朝比奈 靖浩 東京医科歯科大学, 大学院医歯学総合研究科, 寄附講座教授 (00422692)
渡辺 守 (渡邉 守) 東京医科歯科大学, 大学院医歯学総合研究科, 教授 (10175127)
|
|---|
| Project Period (FY) |
2014-04-01 – 2017-03-31
|
| Project Status |
Completed (Fiscal Year 2016)
|
| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
|---|
| Keywords | インターフェロン誘導遺伝子 / C型肝炎ウイルス / GBP-1 / NS5B |
|---|
| Outline of Final Research Achievements |
We have focused on interferon stimulated genes (ISGs), which were involved in efficacy of interferon (IFN)-based therapy for chronic hepatitis C, and investigated regulatory effects of ISG on viral replication in hepatitis C virus (HCV). (1) We have produced newly established cell lines, HCV subgenomic replicon (Huh7/Rep-Feo), which were in-vitro models simulating cellular autonomous replication of HCV genomic RNA. (2) We showed that GBP-1, IFI-6-16, and IFI-27 had directly inhibited subgenomic replication and virus production of HCV. (3) Investigation of molecular interaction of the ISGs with HCV proteins showed that GBP-1 bound HCV-NS5B directly. A protein truncation assay showed that the guanine binding domain of GBP-1 and the finger domain of NS5B were involved in the interaction.
|
