Host factors involved in HBV cccDNA maintenance
Project/Area Number |
26460993
|
Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Gastroenterology
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Research Institution | Kanazawa University |
Principal Investigator |
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Project Period (FY) |
2014-04-01 – 2017-03-31
|
Project Status |
Completed (Fiscal Year 2016)
|
Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
Keywords | B型肝炎ウイルス |
Outline of Final Research Achievements |
Covalently closed circular DNA (cccDNA) forms a template for the replication of hepatitis B virus (HBV). Despite the crucial role of cccDNA in viral persistence, little is known about the host factors that target this viral intermediate. Recent studies have revealed that AID/APOBEC3 cytidine deaminase family members can induce C-to-U hypermutation on viral genome and restrict viral replication. Uracil residues in DNA are removed by base excision repair (BER) enzyme, uracil DNA glycosylase (UNG), when cytidine deamination is occurred in host genome. We investigated whether uracil residues were generated in HBV cccDNA by APOBEC3G and removed by UNG using in vitro. We found that IFNγ stimulation of hepatocyte cell lines induced the endogenous APOBEC3G expression and HBV cccDNA hypermutation. When UNG activity was inhibited, the IFNγ-mediated hypermutation of cccDNA was enhanced. Our result indicate that BER pathway cancels the mutations of the cccDNA generated by APOBEC proteins.
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Report
(4 results)
Research Products
(11 results)
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[Journal Article] APOBEC3A Associates with Human Papillomavirus Genome Integration in Oropharyngeal Cancers2017
Author(s)
Satoru Kondo, Kousho Wakae, Naohiro Wakisaka, Yosuke Nakanishi, Kazuya Ishikawa, Takeshi Komori, Makiko Moriyama-Kita, Kazuhira Endo, Shigeyuki Murono, Zhe Wang,, Kouichi Kitamura, Tomoaki Nishiyama, Katsushi Yamaguchi, Shuji Shigenobu, Masamichi Muramatsu, and Tomokazu Yoshizaki
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Journal Title
Oncogene.
Volume: 36
Issue: 12
Pages: 1687-1697
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] TGF-beta Suppression of HBV RNA through AID-Dependent Recruitment of an RNA Exosome Complex2015
Author(s)
Liang, G., Liu, G., Kitamura, K., Wang, Z., Chowdhury, S., Monjurul, A. M., Wakae, K., Koura, M., Shimadu, M., Kinoshita, K., and Muramatsu, M.
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Journal Title
PLoS Pathog.
Volume: 11
Issue: 4
Pages: e1004780-e1004780
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Detection of hypermutated human papillomavirus type 16 genome by Next-Generation Sequencing.2015
Author(s)
Wakae K, Aoyama S, Wang Z, Kitamura K, Liu G, Monjurul AM, Koura M, Imayasu M, Sakamoto N, Nakamura M, Kyo S, Kondo S, Fujiwara H, Yoshizaki T, Kukimoto I, Yamaguchi K, Shigenobu S, Nishiyama T, Muramatsu M.
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Journal Title
Virology
Volume: 485
Pages: 460-466
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] APOBEC3A and 3C decrease human papillomavirus 16 pseudovirion infectivity.2015
Author(s)
Ahasan MM, Wakae K, Wang Z, Kitamura K, Liu G, Koura M, Imayasu M, Sakamoto N, Hanaoka K, Nakamura M, Kyo S, Kondo S, Fujiwara H, Yoshizaki T, Mori S, Kukimoto I, Muramatsu M.
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Journal Title
Biochem Biophys Res Commun.
Volume: 457
Issue: 3
Pages: 295-9
DOI
Related Report
Peer Reviewed / Open Access
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