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Analysis of hepatic stem and progenitor cells in the tumor microenvironment of liver metastasis: For cancer metastasis suppression

Research Project

Project/Area Number 26460997
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Gastroenterology
Research InstitutionGifu University

Principal Investigator

Suetsugu Atsushi  岐阜大学, 医学部附属病院, 助教 (30340079)

Co-Investigator(Kenkyū-buntansha) 青木 仁美  岐阜大学, 大学院医学系研究科, 講師 (10550361)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords肝癌 / 幹細胞 / 癌微小環境
Outline of Final Research Achievements

Cancer cells form microenvironment in primary and metastatic tumor. The cancer-associated cells play an important role. Cancer-associated fibroblast cells and tumor-associated macrophages are important in the liver metastasis. Cancer cells shed exosomes and transfer exsomal molecules to fibroblast cells during tumor progression. We confirmed that cancer cells release exomes into the circulation of mice with liver metastasis. We collected blood of mice and ditected exosomes. Imaging of exosomes can contribute to understanding the role of intercellular communication in tumor progression. Tumor-drived exosomes have been viewed as a mediater to condition tissues and prepare niches for metastasis.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (39 results)

All 2017 2016 2015 2014 2013

All Journal Article (25 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 24 results) Presentation (13 results) (of which Int'l Joint Research: 4 results) Book (1 results)

  • [Journal Article] Genetic recombination between stromal and cancer cells results in highly malignant cells identified by color-coded imaging in a mouse lymphoma model.2017

    • Author(s)
      Nakamura M, Suetsugu A, Hasegawa K, Matsumoto T, Aoki H, Kunisada T, Shimizu M, Saji S, Moriwaki H, Hoffman RM.
    • Journal Title

      J Cell Biochem

      Volume: - Issue: 12 Pages: 4216-4221

    • DOI

      10.1002/jcb.26068

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Color-coded live imaging of heterokaryon formation and nuclear fusion of hybridizing cancer cells.2016

    • Author(s)
      Suetsugu A, Matsumoto T, Hasegawa K, Nakamura M, Kunisada T, Shimizu M, Saji S, Moriwaki H, Bouvet M, Hoffman RM.
    • Journal Title

      Anticancer Res

      Volume: 36 Pages: 3827-3831

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Color-coded imaging of syngeneic orthotopic malignant lymphoma interacting with host stromal cells during metastasis.2016

    • Author(s)
      Matsumoto T, Suetsugu A, Hasegawa K, Nakamura M, Aoki H, Kunisada T, Tsurumi H, Shimizu M, Hoffman RM.
    • Journal Title

      Anticancer Res

      Volume: 36 Pages: 1473-1478

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A mouse model of fluorescent protein-expressing disseminated peritoneal lymphoma for fluorescence-guided surgery.2016

    • Author(s)
      Matsumoto T, Suetsugu A, Hasegawa K, Nakamura M, Shibata Y, Aoki H, Kunisada T, Tsurumi H, Shimizu M, Bouvet M, Hoffman RM.
    • Journal Title

      Anticancer Res

      Volume: 36 Pages: 4483-4487

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Imaging nuclear-cytoplasmic dynamics in primary and metastatic colon cancer in nude mice.2016

    • Author(s)
      Hasegawa K, Suetsugu A, Nakamura M, Matsumoto T, Aoki H, Kunisada T, Bouvet M, Shimizu M, Hoffman RM.
    • Journal Title

      Anticancer Res

      Volume: 36 Pages: 2113-2117

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Color-coded imaging enables fluorescence-guided surgery to resect the tumor along with the tumor microenvironment in a syngeneic mouse model of EL-4 lymphoma.2016

    • Author(s)
      Hasegawa K, Suetsugu A, Nakamura M, Matsumoto T, Kunisada T, Shimizu M, Saji S, Moriwaki H, Bouvet M, Hoffman RM.
    • Journal Title

      Anticancer Res

      Volume: 36 Pages: 4443-4448

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Angiotensin II type I receptor blocker, Losartan, inhibits fibrosis in liver by suppressing TGF-beta1 production.2016

    • Author(s)
      Ogata H, Noguchi H, Ohtsubo T, Liao J, Kohara H, Yamada K, Murahashi M, Hijikata Y, Suetsugu A, Hoffman RM, Tani K.
    • Journal Title

      Integr Mol Med

      Volume: 3 Pages: 520-523

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Rapid skeletal muscle wasting predicts worse survival in patients with liver cirrhosis.2015

    • Author(s)
      Hanai T, Shiraki M, Ohnishi S, Miyazaki T, Ideta T, Kochi T, Imai K, Suetsugu A, Takai K, Moriwaki H, Shimizu M.
    • Journal Title

      Hepatol Res

      Volume: 印刷中 Issue: 8 Pages: 743-751

    • DOI

      10.1111/hepr.12616

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Recruitment of Cancer-Associated Fibroblasts and Blood Vessels by Orthotopic Liver Tumors Imaged in Red Fluorescent Protein (RFP) Transgenic Nude Mice.2015

    • Author(s)
      Suetsugu A, Hiroshima Y, Matsumoto T, Hasagawa K, Nakamura M, Shimizu M, Saji S, Moriwaki H, Bouvet M, Hoffman RM.
    • Journal Title

      Anticancer Res

      Volume: 35 Pages: 5821-5825

    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Journal Article] Impact of serum glycosylated Wisteria floribunda agglutinin positive Mac-2 binding protein levels on liver functional reserves and mortality in patients with liver cirrhosis.2015

    • Author(s)
      Hanai T, Shiraki M, Ohnishi S, Miyazaki T, Ideta T, Kochi T, Imai K, Suetsugu A, Takai K, Shimizu M, Moriwaki H.
    • Journal Title

      Hepatol Res

      Volume: 45 Issue: 11 Pages: 1083-1090

    • DOI

      10.1111/hepr.12473

    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Journal Article] Skeletal muscle depletion predicts the prognosis of patients with hepatocellular carcinoma treated with sorafenib.2015

    • Author(s)
      Imai K, Takai K, Hanai T, Ideta T, Miyazaki T, Kochi T, Suetsugu A, Shiraki M, Shimizu M.
    • Journal Title

      Int J Mol Sci

      Volume: 16 Issue: 5 Pages: 9612-9624

    • DOI

      10.3390/ijms16059612

    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Journal Article] A Color-coded Imageable Syngeneic Mouse Model of Stromal-cell Recruitment by Metastatic Lymphoma.2015

    • Author(s)
      Matsumoto T, Suetsugu A, Shibata Y, Nakamura N, Aoki H, Kunisada T, Tsurumi H, Shimizu M, Hoffman RM.
    • Journal Title

      Anticancer Res

      Volume: 35 Pages: 4647-54

    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Journal Article] Color-coding cancer and stromal cells with genetic reporters in a patient-derived orthotopic xenograft (PDOX) model of pancreatic cancer enhances fluorescence-guided surgery.2015

    • Author(s)
      Yano S, Hiroshima Y, Maawy A, Kishimoto H, Suetsugu A, Miwa S, Toneri M, Yamamoto M, Katz MH, Fleming JB, Urata Y, Tazawa H, Kagawa S, Bouvet M, Fujiwara T, Hoffman RM.
    • Journal Title

      Cancer Gene Ther

      Volume: 22 Issue: 7 Pages: 344-50

    • DOI

      10.1038/cgt.2015.26

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Imaging the Different Mechanisms of Prostate Cancer Cell-killing by Tumor-targeting Salmonella typhimurium A1-R.2015

    • Author(s)
      Uchugonova A, Zhang Y, Salz R, Liu F, Suetsugu A, Zhang L, Koenig K, Hoffman RM, Zhao M.
    • Journal Title

      Anticancer Res

      Volume: 35 Pages: 5225-9

    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Journal Article] Color-Coded Imaging of Breast Cancer-Metastatic Niche Formation in Nude Mice.2015

    • Author(s)
      Suetsugu A, Momiyama M, Hiroshima Y, Shimizu M, Saji S, Moriwaki H, Bouvet M, Hoffman RM.
    • Journal Title

      J Cell Biochem

      Volume: 116 Issue: 12 Pages: 2730-4

    • DOI

      10.1002/jcb.25227

    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Journal Article] The Use of Living Cancer Cells Expressing Green Fluorescent Protein in the Nucleus and Red Fluorescence Protein in the Cytoplasm for Real-time Confocal Imaging of Chromosome and Cytoplasmic Dynamics During Mitosis.2015

    • Author(s)
      Suetsugu A, Jiang P, Yang M, Yamamoto N, Moriwaki H, Saji S, Hoffman RM.
    • Journal Title

      Anticancer Res

      Volume: 35 Pages: 2553-2557

    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Journal Article] Imaging the Interaction of Pancreatic Cancer and Stellate Cells in the Tumor Microenvironment during Metastasis.2015

    • Author(s)
      Suetsugu A, Snyder CS, Moriwaki H, Saji S, Bouvet M, Hoffman RM.
    • Journal Title

      Anticancer Res

      Volume: 35 Pages: 2545-2551

    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Journal Article] 新規肝線維化マーカー「Mac-2 binding protein 糖鎖修飾異性体(M2BPGi)」のC型肝炎に対するペグインターフェロン・リバビリン併用療法による変化2015

    • Author(s)
      石田秀和、末次 淳、中村真大、田内里奈、中山純里、片野由里子、古田伸行、白上洋平、伊藤弘康、清島 満
    • Journal Title

      臨床病理

      Volume: 63 Pages: 901-906

    • NAID

      40020594726

    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Journal Article] Sarcopenia impairs prognosis of patients with liver cirrhosis.2015

    • Author(s)
      Hanai T, Shiraki M, Nishimura K, Ohnishi S, Imai K, Suetsugu A, Takai K, Shimizu M, Moriwaki H.
    • Journal Title

      Nutrition

      Volume: 31 Issue: 1 Pages: 193-199

    • DOI

      10.1016/j.nut.2014.07.005

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Skeletal muscle depletion is an independent prognostic factor for hepatocellular carcinoma.2015

    • Author(s)
      Iritani S, Imai K, Takai K, Hanai T, Ideta T, Miyazaki T, Suetsugu A, Shiraki M, Shimizu M, Moriwaki H.
    • Journal Title

      J Gastroenterol

      Volume: 50 Issue: 3 Pages: 323-332

    • DOI

      10.1007/s00535-014-0964-9

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Spatial-temporal FUCCI imaging of each cell in a tumor demonstrates locational dependence of cell cycle dynamics and chemoresponsiveness.2014

    • Author(s)
      Yano S, et al.
    • Journal Title

      Cell Cycle

      Volume: 13 Issue: 13 Pages: 2110-2119

    • DOI

      10.4161/cc.29156

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Efficacy of Salmonella typhimurium A1-R Versus Chemotherapy on a Pancreatic Cancer Patient-Derived Orthotopic Xenograft (PDOX).2014

    • Author(s)
      Hiroshima Y, Zhao M, Maawy A, Zhang Y, Katz MH, Fleming JB, Uehara F, Miwa S, Yano S, Momiyama M, Suetsugu A, Chishima T, Tanaka K, Bouvet M, Endo I, Hoffman RM.
    • Journal Title

      J Cell Biochem

      Volume: 115(7) Issue: 7 Pages: 1254-61

    • DOI

      10.1002/jcb.24769

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Pharmaceutical and nutraceutical approaches for preventing liver carcinogenesis: chemoprevention of hepatocellular carcinoma using acyclic retinoid and branched-chain amino acids.2014

    • Author(s)
      Shimizu M, Shirakami Y, Hanai T, Imai K, Suetsugu A, Takai K, Shiraki M, Moriwaki H.
    • Journal Title

      Mol Nutr Food Res.

      Volume: 58 Issue: 1 Pages: 124-135

    • DOI

      10.1002/mnfr.201300538

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] 劇症肝炎におけるTNF-αを介したNF-κB活性調節の細胞死および細胞生存シグナルへの影響2014

    • Author(s)
      末次 淳, 森脇久隆
    • Journal Title

      肝胆膵

      Volume: 68 Pages: 513-518

    • Related Report
      2014 Research-status Report
  • [Journal Article] Free fatty acid as a marker of energy malnutrition in liver cirrhosis.2013

    • Author(s)
      Hanai T, Shiraki M, Nishimura K, Imai K, Suetsugu A, Takai K, Shimizu M, Naiki T, Moriwaki H.
    • Journal Title

      Hepatol Res.

      Volume: 44 Issue: 2 Pages: 218-228

    • DOI

      10.1111/hepr.12112

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Presentation] Imaging of colon cancer-cell mitosis within liver metastasis in nude mice.2016

    • Author(s)
      Hasegawa K, Suetsugu A, Nakamura M, Matsumoto T, Aoki H, Kunisada T, Shimizu M, Hoffman RM.
    • Organizer
      107th American Association for Cancer Research (AACR) Annual Meeting
    • Place of Presentation
      New Orleans
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Color-coded imaging of the tumor microenvironment of malignant lymphoma in a syngeneic mouse model2016

    • Author(s)
      Matsumoto T, Suetsugu A, Hasegawa K, Nakamura M, Aoki H, Kunisada T, Tsurumi H, Shimizu M, Hoffman RM.
    • Organizer
      107th American Association for Cancer Research (AACR) Annual Meeting
    • Place of Presentation
      New Orleans
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Imaging formation of the tumor microenvironment in orthotopic liver tumors in red fluorescent protein (RFP) transgenic nude mice.2016

    • Author(s)
      Suetsugu A, Hiroshima Y, Matsumoto T, Hasegawa K, Nakamura M, Shimizu M, Saji S, Moriwaki H, Bouvet M, Hoffman RM.
    • Organizer
      107th American Association for Cancer Research (AACR) Annual Meeting
    • Place of Presentation
      New Orleans
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] C型肝炎ウイルス治療前後の肝線維化マーカー“ELF Score”の検討2016

    • Author(s)
      末次 淳, 長谷川恒輔, 華井竜徳, 今井健二, 白木 亮, 高井光治, 石田秀和, 清島 満, 清水雅仁
    • Organizer
      第102回日本消化器病学会総会
    • Place of Presentation
      東京
    • Related Report
      2016 Annual Research Report
  • [Presentation] 当院におけるB型肝炎再活性化対策の実態2015

    • Author(s)
      今井健二、末次 淳、清水雅仁
    • Organizer
      第41回日本急性肝不全研究会
    • Place of Presentation
      熊本
    • Year and Date
      2015-05-20
    • Related Report
      2015 Research-status Report
  • [Presentation] Development of a syngeneic metastatic mouse model of malignant lymphoma2015

    • Author(s)
      Takuro Matsumoto, Atsushi Suetsugu, Yuhei Shibata, Nobuhiko Nakamura, Hitomi Aoki ,Takahiro Kunisada, Masahito Shimizu, Hisashi Tsurumi, Robert M. Hoffman
    • Organizer
      AACR Annual Meeting
    • Place of Presentation
      Philadelphia, USA
    • Year and Date
      2015-04-21
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Hepatitis B virus reactivation in lymphoma patients with rituximab therapy2015

    • Author(s)
      uetsugu A, Tsurumi H, Shibata Y, Matsumoto T, Nakamura N, Shimizu M, Moriwaki H.
    • Organizer
      APASL
    • Place of Presentation
      Istanbul
    • Year and Date
      2015-03-13
    • Related Report
      2014 Research-status Report
  • [Presentation] Imaging exosome transfer from colon cancer cells to stroma at liver metastatic sites2014

    • Author(s)
      Suetsugu A, Homma K, Saji S, Moriwaki H, Osawa Y, Ochiya T, Robert M. Hoffman
    • Organizer
      APDW
    • Place of Presentation
      bali
    • Year and Date
      2014-11-24
    • Related Report
      2014 Research-status Report
  • [Presentation] Color-coded chemotherapy: S/G2-phase-trapping by methioninase pre-treatment, indicated by FUCCI imaging, enables highly effective cancer chemotherapy.2014

    • Author(s)
      Yano S, Tome Y, Michelle Digman, Momiyama M, Suetsugu A, Enrico Gratton, Robert Hoffman.
    • Organizer
      Experimental Biology 2014 FASEB J
    • Place of Presentation
      San Diego, CA, USA
    • Year and Date
      2014-04-26
    • Related Report
      2014 Research-status Report
  • [Presentation] Real-time FUCCI imaging of cell cycle phase in tumors demonstrates why cancer chemotherapy is not effective in solid cancers2014

    • Author(s)
      Yano S, Tome Y, Yong Zhang, Hiroshima Y, Miwa S, Uehara F, Suetsugu A, Kishimoto H, Tazawa H, Ming Zhao, Fujiwara T, Robert M. Hoffman.
    • Organizer
      AACR Annual Meeting
    • Place of Presentation
      San Diego, CA, USA
    • Year and Date
      2014-04-09
    • Related Report
      2014 Research-status Report
  • [Presentation] elomerase-specific GFP-expressing adenovirus enables fluorescence-guided surgery (FGS) for patient-derived orthotopic xenograft (PDOX) in nude mice2014

    • Author(s)
      Yano S, Hiroshima Y, Ali Maawy, Matthew H.G. Katz, Jason B. Fleming, Kishimoto H, Suetsugu A, Uehara F, Miwa S, Tazawa H, Michael Bouvet, Fujiwara T, Robert M. Hoffman.
    • Organizer
      AACR Annual Meeting
    • Place of Presentation
      San Diego, CA, USA
    • Year and Date
      2014-04-07
    • Related Report
      2014 Research-status Report
  • [Presentation] Real-time imaging of exosomes dynamic of cross-talking and cell trafficking in 3D Gelfoam® histoculture.2014

    • Author(s)
      Yamamoto M, Miwa S, Hiroshima Y, Yano S, Uehara F, Matsumoto Y, Suetsugu A, Kimi Homma, Robert M. Hoffman.
    • Organizer
      AACR Annual Meeting
    • Place of Presentation
      San Diego, CA, USA
    • Year and Date
      2014-04-07
    • Related Report
      2014 Research-status Report
  • [Presentation] Imaging of stromal cells during hepatocellular metastasis formation in the RFP-MUP-uPA mouse.2014

    • Author(s)
      Suetsugu A, Ogata H, Hiroshima Y, Momiyama M, Osawa Y, Moriwaki H, Saji S, Michael Karin, Robert M. Hoffman.
    • Organizer
      AACR Annual Meeting
    • Place of Presentation
      San Diego, CA, USA
    • Year and Date
      2014-04-06
    • Related Report
      2014 Research-status Report
  • [Book] 消化器病診療第2版2014

    • Author(s)
      末次 淳,森脇久隆.
    • Total Pages
      528
    • Publisher
      医学書院
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2018-03-22  

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