Molecular mechanism of hepatitis C virus-induced lipid droplet formation
| Project/Area Number |
26461003
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | Kobe University |
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Principal Investigator |
SHOJI Ikuo 神戸大学, 医学研究科, 教授 (40356241)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | HCV / 脂肪滴 / NS3/4A プロテアーゼ / 脂肪化 / 肝炎 / 肝癌 / 肝脂肪化 / ウイルス増殖 |
|---|
| Outline of Final Research Achievements |
(1) SPG20 was cleaved in HCVcc-infected Huh-7.5 cells. (2) Transient expression of HCV NS3/4A resulted in cleavage of SPG20. (3) Inactive mutant NS3/4A did not cleave SPG20. (4) NS3/4A inhibitor inhibited cleavage of SPG20, whereas NS5A inhibitor did not inhibit cleavage of SPG20. (5) Treatment of the HCVcc-infected Huh-7.5 cells decreased the size of lipid droplets. Taken together, our results suggest that HCV NS3/4A cleaves SPG20 thereby promoting enlargement of lipid droplet in HCV-infected cells.
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Report
(4 results)
Research Products
(14 results)
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[Journal Article] TRC8-dependent degradation of hepatitis C virus immature core protein regulates viral propagation and pathogenesis.2016
Author(s)
Aizawa S., Okamoto T., Sugiyama Y., Kouwaki T., Ito A., Suzuki T., Ono C., Fukuhara T., Yamamoto M., Okochi M., Hiraga N., Imamura M., Chayama K., Suzuki R., Shoji I., Moriishi K., Moriya K., Koike K., and Matsuura Y.
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Journal Title
Nat. Commun.
Volume: 7
Issue: 1
Pages: 1-12
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Nitrosative stress induces peroxiredoxin 1 ubiquitination during ischemic insult via E6AP activation in endothelial cells both in vitro and in vivo2014
Author(s)
Tao RR, Wang H, Hong LJ, Huang JY, Lu YM, Liao MH, Ye WF, Lu NN, Zhu DY, Huang Q, Fukunaga K, Lou YJ, Shoji I, Wilcox CS, Lai EY, Han F
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Journal Title
Antioxid Redox Signal
Volume: (in press)
Issue: 1
Pages: 1-16
DOI
Related Report
Peer Reviewed / Open Access
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