A Series of microRNA in Related to Progression of Non-Alcoholic Fatty Liver Disease

• Project/Area Number: 26461005
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Gastroenterology
• Research Institution: Tottori University
• Principal Investigator: Koda Masahiko 鳥取大学, 医学部, 医員 (20243389)
• Co-Investigator(Kenkyū-buntansha): 法正 恵子 鳥取大学, 医学部, 助教 (20379621) ; 岡本 欣也 鳥取大学, 医学部附属病院, 助教 (20464282) ; 下廣 寿 鳥取大学, 医学部, 講師 (90583758) ; 杉原 誉明 鳥取大学, 医学部附属病院, 助教 (60753853) ; 的野 智光 鳥取大学, 医学部附属病院, 助教 (60571841)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 非アルコール性脂肪肝炎 / 非アルコール性脂肪肝 / miRNA / microRNA / NASH

Outline of Final Research Achievements

The aim was to use animal models and human samples to examine the relationship between miRNA expression and each type of NAFLD (SS and NASH).DD Shionogi, Fatty Liver Shionogi (FLS) and FLS ob/ob mice were used as models for normal control, SS and NASH, respectively. Fourteen miRNAs showed clear expression differences among liver tissues from SS, NASH, and control mice with good reproducibility. Among these NAFLD candidate miRNAs, seven showed similar expression patterns and were upregulated in both SS and NASH; these seven candidate miRNAs mapped to an miRNA cluster in the 14q32.2 maternally imprinted region delineated by delta-like homolog 1 and type III iodothyronine deiodinase (Dlk1-Dio3 mat). Serum samples from patients with SS or NASH differed markedly with regard to expression of the putative Dlk1-Dio3 mat miRNAs, and these differences accurately corresponded with NAFLD diagnosis.The expression profiles of seven miRNAs in 14q32.2 mat have high potential as biomarkers for NAFLD.

Research Products

• [Presentation] Maternal imprinted Dlk1-Dio3 miRNAクラスターのNASH発症、進展に及ぼす影響 (2016)
  • Author(s): 岡本欣也、孝田雅彦、杉原誉明
  • Organizer: 第52回 日本肝臓学会総会
  • Place of Presentation: ホテルニューオオタニ幕張（千葉県千葉市）
  • Year and Date: 2016-05-19
• [Presentation] 非アルコール性脂肪肝炎発症に関連するmiRNAの網羅的検索 (2014)
  • Author(s): 岡本欣也、杉原誉明、孝田雅彦、岡本敏明、斧山 巧、三好謙一、木科学、村脇義和
  • Organizer: 第18回日本肝臓学会大会
  • Place of Presentation: 神戸国際会議場、神戸市
  • Year and Date: 2014-10-25
• [Presentation] MicroRNA Expression Profile in Simple Steatosis and Non-Alcoholic Steatohepatitis (2014)
  • Author(s): Okamoto K, Okamoto T, Onoyama T,Miyoshi K,Kishina M, Kato J, Tokunaga S, Sugihara T, Hara Y, Koda M, Hino K, Murawaki Y
  • Organizer: 22th. United European Gastroenterology week
  • Place of Presentation: Austria Center Vienna,オーストリア
  • Year and Date: 2014-10-20