| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
In this study, we determined whether endogenous antigen presentation by hepatocytes or cross-presentation by bone marrow derived cells is more important in the induction of hepatitis B virus (HBV)-specific T cell responses. To do so, we employed transgenic mice that replicate HBV at high level in the liver (HBV-Tg mice) to generate HBV-Tg mice whose MHC class I expression is absent on bone marrow derived cells, or parenchymal cells. We then adoptively transferred HBV-specific T cells, and monitored their responsiveness. Our data suggest that cross-presentation by hematopoietic cells is required for the optimum expansion of HBV-specific T cells, but endogenous antigen presentation by non-hematopoietic cells, presumably hepatocytes, is far more essential in the expansion and cytolytic differentiation of HBV-specific T cells.
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