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Comprehensive screening of genes resistant to Sorafenib in Hepatocellular carcinoma

Research Project

Project/Area Number 26461019
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Gastroenterology
Research InstitutionKeio University

Principal Investigator

Abe Yuta  慶應義塾大学, 医学部(信濃町), 講師 (70327526)

Co-Investigator(Renkei-kenkyūsha) Hayashida Tetsu  慶應義塾大学, 医学部, 講師 (80327543)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords肝細胞癌 / 化学療法 / ソラフェニブ / 放射線治療 / 薬剤耐性 / 放射線耐性
Outline of Final Research Achievements

We used a novel method involving transposons to screen and identify drug-resistant genes. Transposons are DNA sequences that move from one location on the gene to another. A modified piggyBac transposon was designed as an insertion mutagen, and a cytomegalovirus (CMV) promoter sequence was added to induce strong transcription. When the transposon is inserted to the upstream of a certain gene, the gene will be overexpressed while when intserted down or intragenically, it will be downregulated. After establishing a transposon-tagged cell library, we treated cell lines derived from Hepatocellular carcinoma. We performed splinkerette PCR and TOPO cloning on the resistant colonies. Bacterial colonies were sequenced, and next-generation sequencing was used to identify the overexpressed/downregulated sequences as candidate genes for Sorafenib resistance. We identified several candidate genes from 30 resistant colonies.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (1 results)

All 2016

All Presentation (1 results)

  • [Presentation] トランスポゾンによる薬剤・放射線耐性遺伝子の新規網羅的解析法とその運用2016

    • Author(s)
      林田 哲
    • Organizer
      第49回制癌剤適応研究会
    • Place of Presentation
      会津東山温泉(福島県会津若松市)
    • Related Report
      2016 Annual Research Report

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Published: 2014-04-04   Modified: 2018-03-22  

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