| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Outline of Final Research Achievements |
Primary bile acids biosynthesized in the liver are metabolized to secondary bile acids by specific microbes in human intestine. We hypothesized that the individual bile acid profile in feces, and possibly in serum, could be a convenient biomarker for the gut microbiota. The fecal microbiota profile in patients with gastrointestinal diseases and healthy controls were determined by terminal restriction fragment length polymorphism (T-RFLP) analysis. Bile acid compositions in serum and feces from the same subjects were also quantified by HPLC-MS/MS. Our results showed that the fecal proportions of some bacterial clusters were significantly and positively correlated with not only fecal but also serum proportions of specific bile acids. Therefore, the serum bile acid profile could be a convenient surrogate marker for the intestinal proportion of a certain bacterial cluster.
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