Application of serum bile acid profile as a new marker for gut microbiota

• Project/Area Number: 26461022
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Gastroenterology
• Research Institution: Tokyo Medical University
• Principal Investigator: Honda Akira 東京医科大学, 医学部, 教授 (10468639)
• Co-Investigator(Kenkyū-buntansha): 池上 正 東京医科大学, 医学部, 教授 (40439740) ; 宮崎 照雄 東京医科大学, 医学部, 講師 (60532687)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
• Keywords: 胆汁酸 / LC-MS/MS / 腸内細菌叢 / 消化器疾患

Outline of Final Research Achievements

Primary bile acids biosynthesized in the liver are metabolized to secondary bile acids by specific microbes in human intestine. We hypothesized that the individual bile acid profile in feces, and possibly in serum, could be a convenient biomarker for the gut microbiota. The fecal microbiota profile in patients with gastrointestinal diseases and healthy controls were determined by terminal restriction fragment length polymorphism (T-RFLP) analysis. Bile acid compositions in serum and feces from the same subjects were also quantified by HPLC-MS/MS. Our results showed that the fecal proportions of some bacterial clusters were significantly and positively correlated with not only fecal but also serum proportions of specific bile acids. Therefore, the serum bile acid profile could be a convenient surrogate marker for the intestinal proportion of a certain bacterial cluster.

Research Products

• [Journal Article] Protective effect of agaro-oligosaccharides on gut dysbiosis and colon tumorigenesis in high-fat diet-fed mice (2016)
  • Author(s): Higashimura Y, Naito Y, Takagi T, Uchiyama K, Mizushima K, Ushiroda C, Ohnogi H, Kudo Y, Yasui M, Inui S, Hisada T, Honda A, Matsuzaki Y, Yoshikawa T
  • Journal Title: Am. J. Physiol. Gastrointest. Liver Physiol. Volume: 310 Issue: 6 Pages: G367-G375
  • DOI: 10.1152/ajpgi.00324.2015
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] 腸内細菌叢と血中胆汁酸（血中胆汁酸様相から腸内細菌の変化を知る） (2015)
  • Author(s): 本多 彰
  • Journal Title: たんじゅうさん Volume: 14 Pages: 18-18
• [Presentation] Detection of gut dysbiosis due to reduced Clostridium subcluster XIVa based on the serum bile acid profile (2017)
  • Author(s): Murakami M, Iwamoto J, Miyazaki T, Monma T, Shoichiro Y, Ikegami T, Matsuzaki Y, Honda A
  • Organizer: Digestive Disease Week 2017
  • Place of Presentation: Chicago, USA
  • Int'l Joint Research
• [Presentation] 齧歯類におけるミュリコール酸合成酵素の探索（その１） (2015)
  • Author(s): 本多 彰，宮崎照雄，平山 剛，宮本和宜，池上 正，松崎靖司
  • Organizer: 第37回胆汁酸研究会
  • Place of Presentation: 橫浜
  • Year and Date: 2015-11-07
• [Presentation] 腸内環境マーカーとしての末梢血胆汁酸分画測定 (2014)
  • Author(s): 本多 彰，池上 正，岩本淳一，宮崎照雄，松崎靖司
  • Organizer: 日本抗加齢医学会総会
  • Place of Presentation: 大阪
  • Year and Date: 2014-06-08
  • Invited