| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Outline of Final Research Achievements |
We have demonstrated that a mutant isoform (269A) of the SxxSS-harboring TCF-4K robustly expressed WNT5A at both mRNA and protein levels in human liver cancer cells. ChIP assay revealed that the mutant TCF-4 could directly bind to promoter region of WNT5A gene, suggesting the motif contributed to regulate the gene expression. Knockdown of WNT5A resulted in upregulation of the EMT regulator SLUG. These findings suggest that TCF-4 regulates WNT5A expression in a SxxSS motif-dependent manner, thereby contributing to phenotypic changes, including EMT. I addition, HIPK-2 was suggested to be responsible for phosphorylation of serine residues of SxxSS.
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