| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Outline of Final Research Achievements |
The aim of this study was to identify a new candidate gene responsible for alcoholic pancreatitis. Genomic DNA was prepared from a total of 560 patients with pancreatitis and 439 control subjects. The SureSelect Human All Exon kit was used for the analysis of all exons in 16 patients with alcoholic pancreatitis. There were about 1,340,000 variants in total of the 16 samples. We compared frequency of the residual variants from that of control samples by Human Genetic Variation Browser. Statistically significant difference was observed in the frequency of p.S89R variant in ring finger protein 113B between patients with alcoholic pancreatitis (14.3%; 31/217) and control subjects (6.6%; 29/410) (OR 2.4 p=0.002). The frequency of this variant in patients with non-alcoholic pancreatitis (8.2%; 28/343) was not statistically different from that in controls. Next generation sequencing might become the new strategy to identify the candidate genes for alcoholic pancreatitis.
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